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Effect of Exogenous NO on Expression of Tomato Antioxidant Enzyme Gene Under Tomato Yellowing Leaf Curl Virus Stress Jincheng LUO, Xiaolin ZHU, Xiaohong WEI, Xian WANG, Baoqiang WANG, Xuefen DU Journal of Agricultural Science and Technology    2025, 27 (2): 125-135.   DOI: 10.13304/j.nykjdb.2023.0647 Abstract （2569）   HTML （5）    PDF （2999KB）（417）       Knowledge map    Save In order to investigate the effect of exogenous nitric oxide （NO） on the expression of tomato antioxidant gene under the stress of tomato yellow leaf curl virus （TYLCV）， the susceptible tomato Jinpeng 1 was used as the experimental material. Transcriptome sequencing， fluorescent qRT-PCR and bioinformatics analysis were performed under control （CK）， TYLCV （TY） and NO+TYLCV （NO+TY） treatments. The results showed that a total of 55 antioxidase-coding genes were selected in the tomato genome， among which the antioxidase-containing genes with the highest number of exons in different subcellular regions were significantly responsive to TYLCV stress. The number of NO-mediated antioxidant enzyme coding genes in different subcellular compartms showed chloroplast>cell membrane>cytoplasm>peroxisome>vacuole， in which the expression levels of Chl Cu-Zn SOD， Chl MR2， Chl GR， Per MR， Pla CAT1 and Pla CAT7 were significantly up-regulated， and the expression levels of Chl Fe SOD1， Chl Fe SOD2， Cyt GPX， Cyt APX1， Cyt APX2 L-5， Pla CAT3， Pla CAT8 and Vac CAT were significantly down-regulated. Fluorescence qRT-PCR verified that Chl GR， Min Mn SOD and Per CAT2 responded to TYLCV， and Chl Cu-Zn SOD， Pla CAT7， Pla CAT8 and Cyt APX2 L-5 responded to TYLCV and NO. Above results provided theoretical basis for studying the mechanism of NO in improving tomato disease resistance. Table and Figures | Reference | Related Articles | Metrics

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Physiological Response of Tobacco Infected by Phytophthora to Trichoderma harzianum Inoculation Sequence Zhichao REN, Yaohui MU, Xuyang YAO, Shue LI, Yongfeng ZHANG, Tianbao REN, Guoshun LIU, Quanyu YIN Journal of Agricultural Science and Technology    2025, 27 (1): 155-164.   DOI: 10.13304/j.nykjdb.2023.0544 Abstract （2508）   HTML （2）    PDF （1598KB）（382）       Knowledge map    Save To explore the physiological response of tobacco plant infected by Phytophthora to Trichoderma harzianum application sequence， the pot experiments were carried out with different treatments including inoculated with Phytophthora spore suspension （T1）， inoculated with T. harzianum spore suspension （T2）， inoculated with Phytophthora spore suspension first and then inoculated with T. harzianum spore suspension （T3）， inoculated with T.harzianum spore suspension first and then inoculated with Phytophthora spore suspension （T4）， inoculated with Phytophthora spore suspension and T. harzianum spore suspension at the same time （T5）， and clear water （CK） as controls. The effects of T. harzianum application sequence on biological characteristics， physiological characteristics and induced resistance of tobacco infected by Phytophthora were analyzed. The results showed that， in the growth of tobacco plants， the agronomic characters of tobacco plants with T4 treatment increased the most， and the dry and fresh weight of underground parts increased by 103.73% and 8.30% compared with CK. In the physiological characteristics of tobacco plants， T. harzianum could reduce the inhibition of Phytophthora on photosynthetic pigment accumulation and root activity of tobacco plants， and significantly increase both of them， and it could also strengthen the activities of enzymes related to carbon and nitrogen metabolism in tobacco plants. After transplanting 21 d， the activities of nitrate reductase and glutamine synthetase with T4 treatment increased by 13.14% and 6.40%， respectively. In the control effect， T4 treatment had the lowest incidence and disease index， which were 39.39% and 13.89%， and the highest antioxidant enzyme activity. In conclusion， there were significant differences in the physiological response of tobacco plants to the inoculation sequence of T. harzianum. In production， T. harzianum should be inoculated in advance to promote the growth and development of tobacco and inhibit the incidence of black shank disease. Above results laid a theoretical foundation for the regulation of tobacco growth and development and the comprehensive prevention and control of black shank. Table and Figures | Reference | Related Articles | Metrics

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Research Progress on Biocontrol Mechanism and Synergistic Disease Prevention of Trichoderma Xiao WEI, Chunxia CAO, Daye HUANG, Jingwu YAO, Qinfeng YUAN Journal of Agricultural Science and Technology    2024, 26 (11): 126-135.   DOI: 10.13304/j.nykjdb.2023.0524 Abstract （2480）   HTML （9）    PDF （551KB）（90）       Knowledge map    Save Trichoderma is a widely distributed filamentous fungus and widely used as a biological fungicide to control a variety of plant diseases in agriculture due to its excellent biocontrol potential. Trichoderma can reduce plant diseases caused by pathogens through different direct mechanisms （mycoparasitism and the production of cell wall degrading enzymes， antibiosis， competition for space or nutrients） or indirect mechanisms （induction of plant defense ）. Compound biocontrol agents have received increasing attention in recent years. Trichoderma can be used in combination with other biocontrol products to produce better biocontrol effects because of its resistance to chemical pesticides and compatibility with rhizosphere microorganisms. The related mechanisms of Trichoderma， including parasitism， antibiosis， nutrient competition and induction of plant resistance were summarized， and the current research progress were discussed. In addition， the research progress of synergistic disease prevention between Trichoderma and other microorganisms or non-biological （chemical fungicides， other substances） with disease prevention effect was reviewed， and the current research status and the subsequent development prospects of Trichoderma as a biological fungicide were explored. Table and Figures | Reference | Related Articles | Metrics

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Identification and Sensitivity to Fungicides of Pathogen Species of Hass Avocado Fruit Anthracnose Jinlin LIU, Yingying CHEN, Xiaomei LAN, Haiyong CHEN, Pengsheng LI, Bi QIN, Ye YANG Journal of Agricultural Science and Technology    2025, 27 (1): 138-146.   DOI: 10.13304/j.nykjdb.2023.0702 Abstract （2344）   HTML （1）    PDF （3306KB）（25）       Knowledge map    Save To identify the pathogen of anthracnose in avocado and screen the suitable fungicides， fruit samples of Hass avocado suspected anthracnose disease from Danzhou city， Hainan province were sampled. And the pathogen was isolated and identified. The pathogenicity of strain was confirmed according to Koch’s postulate. And the sensitivity of pathogenic strain to 9 fungicides were evaluated in vitro using mycelium radial growth assay. The results showed that the 4 pathogenic strains were identified as Colletotrichum siamense according to the morphological and phylogenetic analyses of multi-locus sequences. All of 4 strains showed virulence and high pathogenicity to avocado fruits. Among 9 fungicides， prochloraz was the most effective with the mean effective inhibitory medium concentration （EC50） values of 0.11 mg·L-1， and azoxystrobin， difenoconazole， pyrazoxystrobin， penthiopyrad and carbendazim followed with mean EC50 values ranged from 2.09 to 4.29 mg·L-1. So these 6 above-mentioned fungicides exhibited strong inhibitory activities against the mycelial growth of C. siamense， which could be used as candidate agents for the control of avocado anthracnose. Above results should help in developing strategies for the management and control of anthracnose in avocado. Table and Figures | Reference | Related Articles | Metrics

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Effects of Bacillus thuringiensi on Intestinal Bacteria in Ectropis obliqua Ruoheng JIN, Xiaoyu LI, Jingwu YAO, Beibei WANG, Chunxia CAO, Daye HUANG Journal of Agricultural Science and Technology    2025, 27 (2): 141-149.   DOI: 10.13304/j.nykjdb.2023.0794 Abstract （2257）   HTML （1）    PDF （3110KB）（79）       Knowledge map    Save Ectropis obliqua is one of the most important pests on tea， which has brought serious economic losses to the tea industry. Bacillus thuringiensis （Bt）?， as a key biological insecticide for the control of E. obliqua， plays an important role in the green prevention and control of diseases and pests in tea gardens. To clarify the effect of Bt treatment on intestinal bacteria of E. obliqua larvae， the Illumina platform using 16S rDNA high-throughput sequencing was applied to compare the structure and population diversity of intestinal commensal community in E. obliqua larvae treated with different dosage of Bt suspensions. The results showed that， compared with CK treatment， the intestinal bacterial composition of E. obliqua larvae treated with lower dosage of Bt did not change significantly， but those treated with higher dosage of Bt had significant effect on the composition of intestinal bacteria in E. obliqua larvae. And the treatments with higher dosage of Bt significantly increased the diversity and abundance of intestinal bacteria in E. obliqua larvae， which indicated that intestinal microorganisms were involved in the response of E. obliqua larvae to Bt infection. Above results provided basis for further exploring the insecticidal mechanism of Bt， and provided new ideas for improving the virulence of Bt. Table and Figures | Reference | Related Articles | Metrics

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Symptoms and Pathogen Identification of Ficus carica Bacterial Leaf Spot Disease in Fujian Mengting JI, Ling ZHU, Xiaohua LUO, Yuting ZHENG, Shun XIAO, Fangping HU, Xueqing CAI Journal of Agricultural Science and Technology    2025, 27 (1): 147-154.   DOI: 10.13304/j.nykjdb.2023.0546 Abstract （2228）   HTML （0）    PDF （2145KB）（563）       Knowledge map    Save To clarify the taxonomy of the pathogen causing a new bacterial leaf spot disease on fig （Ficus carica） in Fuzhou in 2020， diseased fig leaves were collected as test materials. The bacterial strains were isolated from the diseased leaves by streak plate method， and then the pathogenicity of the strains was determined by tobacco allergic reaction， spray inoculated fig leaves and Koch’s rule. The pathogen was identified based on morphological characteristics， physiological and biochemical determination， and phylogenetic tree analysis using partial sequences of 16S rDNA， gyrB and rpoD. The results showed that 10 bacterial strains were isolated and purified from the diseased leaves， healthy fig leaves demonstrated similar symptoms as the specimen collected from fields， and the identical morphological bacterial strains were re-isolated from diseased fig leaves. Thus， all of the bacterial strains were identified as the causal pathogens of bacterial leaf spot disease by Koch’s rule. The colony morphology was light yellow， round， and flat on the NA medium after 2 d of culture， and the gram staining reaction was negative. The bacterial strains could produce green water-soluble fluorescent on KB medium. The sequencing analysis based on 16S rDNA， gyrB and rpoD showed that the test strains were clustered with Pseudomonas cichorii； and the results of physiological and biochemical characteristics， Biolog phenotypes instrument， and specific primers were similar to the P. cichorii strain 5707， therefore the 10 isolated bacterial strains were identified as P. cichorii. It was the first report that P. cichorii could naturally cause the leaf spot disease in F. carica. Table and Figures | Reference | Related Articles | Metrics

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Global Genetically Modified Crop Industrialization Trends in 2022 Haohui LI, Caiyue LIU, Haiwen ZHANG, Xujing WANG, Qiaoling TANG, Youhua WANG Journal of Agricultural Science and Technology    2023, 25 (12): 6-16.   DOI: 10.13304/j.nykjdb.2023.0756 Abstract （2181）   HTML （85）    PDF （752KB）（1062）       Knowledge map    Save Since the commercialization of genetically modified （GM） crops in 1996， their industrial applications have developed rapidly in worldwide with increasing planting area and crop types， which have significant economic and social benefits. This paper analyzed the global situation of GM crops cultivation in 2022， and the development trends of major countries and regions planting GM crops. In 2022， the planting area of GM crops accounted for about 12% of the total arable land area with an increase of 3.3%. The number of the countries approved to cultivate GM crops increased to 29， and 71 countries and regions approved the commercial application of GM products. Based on the planting area of GM crops， the GM corn and soybean were dominant， and the planting area of the GM crops with multiple traits was also constantly expanding. The industrial applications of GM crops will become new growth driver for agricultural production and economic development， and the applications and effects of GM crops will continuously increase with the iterative upgrading of bioengineering breeding technologies. Table and Figures | Reference | Related Articles | Metrics

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Reference Gene Selection in Plant Real-time Quantitative Reverse Transcription PCR (qRT-PCR) HU Rui-bo, FAN Cheng-ming, FU Yong-fu    2009, 11 (6): 30-36.   Abstract （2150）      PDF （623KB）（2675）       Knowledge map    Save Real-time quantitative RT-PCR (qRT-PCR) technology, with quantitative accuracy, high sensitivity and high-throughput characteristics, has been widely used in gene expression analysis. Based on the relative quantitative analysis, qRT-PCR data must be normalized with one or more proper and stable internal reference genes. House-keeping genes are customarily used as endogenous references for relative quantification. But not a single gene can act as a universal reference reported so far. Most of the traditional housekeeping genes are unable to ensure accurate normalization in qRT-PCR. Based on the tremendous gene-chip expression data and public deposited EST data, new reference genes with superior stability were selected and verified with qRT-PCR. The research progress of reference genes in plant qRT-PCR was reviewed and aspects to be considered in future reference gene selection were also discussed. Related Articles | Metrics

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Application and Prospect of Virus-induced Gene Silencing in Crop Gene Function Research Mengyuan HAO, Qi HANG, Gongyao SHI Journal of Agricultural Science and Technology    2022, 24 (1): 1-13.   DOI: 10.13304/j.nykjdb.2020.0781 Abstract （1977）   HTML （107）    PDF （1362KB）（1102）       Knowledge map    Save With the completion of crop genome sequencing， a large number of genes' functions affecting important agronomic traits of crops are waiting to be discovered. Due to the lack of genetic transformation methods， the research on gene function of most crops is progressing slowly. Virus-induced gene silencing （VIGS） technology does not rely on genetic transformation. Through virus vector inoculation， efficient silencing of targeted genes can be achieved in contemporary plants. VIGS technology is increasingly used in reverse genetics for analysis of gene functions and metabolic pathways in different crops due to its fast onset time， high silencing efficiency， low operating cost， high-throughput and wide application of plants. This article introduced the technical principles and development process of VIGS， systematically summarized the application of VIGS in different crop research， and then summarized and discussed the limitations of existing VIGS applications and several key factors affecting its silence efficiency， and finally the application in future plant biology research was prospected， which would provide reference for its further application and development. Table and Figures | Reference | Related Articles | Metrics

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Genome Editing and Its Applications in Crop Improvement Lei YAN, Jinshan ZHANG, Jiankang ZHU, Lanqin XIA Journal of Agricultural Science and Technology    2022, 24 (12): 78-89.   DOI: 10.13304/j.nykjdb.2022.1030 Abstract （1919）   HTML （26）    PDF （1256KB）（271）       Knowledge map    Save In the context of climate change， increasing world population， decreased farmland as well as frequent outbreaks of natural disasters， global food production is facing unprecedent challenges. CRISPR/Cas-mediated genome editing technologies enable targeted genome modification in a sequence-specific manner， offering great potential in crop improvement. This review aimed to provide an informative summary of the latest development and breakthroughs in CRISPR/Cas-mediated genome editing technologies including gene knockout， base editing， prime editing and gene replacement through homology-directed repair （HDR）， as well as their applications in crop improvement. Finally， we discussed the existing challenges and future prospects in the development of novel genome editing toolkits and their applications in agriculture to ensure global food security and sustainable agricultural development. Table and Figures | Reference | Related Articles | Metrics

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Effect of Chinese Chestnut Yellow Crinkle Disease on Quality of Chestnut Dexuan ZHO, Peng GAO, Xiaolei WEN, Shifeng MU, Suhong GAO, Lina FENG, Weiming SUN, Huixia QI Journal of Agricultural Science and Technology    2025, 27 (2): 136-140.   DOI: 10.13304/j.nykjdb.2023.0844 Abstract （1898）   HTML （2）    PDF （559KB）（20）       Knowledge map    Save In order to comprehensively evaluate the effect of Chinese chestnut yellow crinkle disease on the quality of chestnut nuts， the related indexes of nut quality of trees were determined， and the effect of the disease on nut quality was analyzed. The results showed that， compared with healthy trees， the contents of trace elements in nut of diseased trees were increased， and the contents of amino acids， total phenols and flavonoids were significantly increased by 24.93%， 20.54% and 36.33%， respectively. The chestnut bracts of diseased trees had long and sof spines， uneven color of nuts， the wrinkled surface and the rough texture. The number of grains per bract， the weight of single bract， the full nut rate and the yield per plant of diseased trees were significantly reduced， among them， the yield per plant was reduced by 96.45% compared with the healthy tree. Above results provided a theoretical basis for scientific evaluation of the effect of Chinese chestnut yellow crinkle disease on nut quality. Table and Figures | Reference | Related Articles | Metrics

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Research Progress on Two Component System of Bacteria HAO Yan-hua, ZHANG Wei, CHEN Ming    2012, 14 (2): 67-72.   DOI: 10.3969/j.issn.1008-0864.2012.02.11 Abstract （1872）      PDF （358KB）（4622）       Knowledge map    Save Bacteria needs complex and accurate signal transduction system to adapt to the changes in cellular and environment. Two component systems are abundant in prokaryotes, which consist of a histidine kinase (HK) and a response regulator (RR). They regulate cellular signal transduction pathway, according to phosphorylation and dephosphorylation. This review mainly introduces the progress made in structure, mechanism and function research, and explores the role of two component systems in cell signal transduction. Related Articles | Metrics

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Transcriptome Analysis of Resistance to Sharp Eyespot of Wheat Mediated by Piriformospora indica and Key Genes Screening Shuo SHI, Yu FENG, Liang LI, Rui MENG, Yanze ZHANG, Xiurong YANG Journal of Agricultural Science and Technology    2025, 27 (5): 133-145.   DOI: 10.13304/j.nykjdb.2023.0152 Abstract （1868）   HTML （1）    PDF （8319KB）（193）       Knowledge map    Save Sharp eyespot of wheat is a frequent soil borne disease worldwide， which has serious harm on stable and high yield of wheat. To clarify the induction effect of Piriformospora indica， an endophytic fungus， on wheat resistance to sharp eyespot and to use a biological method which breaking through the traditional chemical control mode to enhance wheat resistance to sharp eyespot， wheat seeds were soaked in P. indica spore solution， followed by inoculation with Rhizoctonia cerealis， and physiological and biochemical indicators and transcriptome results were analyzed. The results showed that pre-colonization of P. indica in wheat could enhance the activity of antioxidant enzymes， alleviate the reduction of relative water content caused by Rhizoctonia cerealis and increase the membrane stability that partially destroyed by the fungi by 39.6%， and could also raise the content of chlorophyll by 32.3%， improving the photosynthesis ability of wheat. The transcriptome analysis revealed that the colonization of P. indica could restore the transcriptome changes caused by pathogenic fungi， induce the expression of related resistance genes， and improved plant biomass by influencing hormonal metabolic pathways. The resistance of wheat to R. cerealis had been comprehensively promoted by various ways and means. Key genes including TraesCS1A02G372300 and TraesCS1B02G393100 etc had been screened based on the transcriptome data， which would provide theoretical and experimental basis for catching on the kernal layer of plant-microbe interaction and acceleration of the breeding work related to disease resistance and yield increase. Table and Figures | Reference | Related Articles | Metrics

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Research Progress on Application of Superoxide Dismutase (SOD) DONG Liang1, HE Yong\|zhi2, WANG Yuan\|liang1*, DONG Zhi\|yang2*    2013, 15 (5): 53-58.   DOI: 10.3969/j.issn.1008-0864.2013.05.08 Abstract （1844）      PDF （1027KB）（3407）       Knowledge map    Save Superoxide dismutase (SOD) catalyzes the disproportionation of univalently reduced oxygen to generate oxygen and hydrogen peroxide. SOD has been widely discovered in living organisms, including animals, plants and microorganisms and has been demonstrated to be an important antioxidant, which protects the cells exposed to the toxicity of oxygen. Recently, SOD has displayed huge application value and development prospect. This paper gave an sumarized SOD′s function, its anti\|oxygen mechanism, types and production mode, etc., and also expounds the application situation and development potential of SOD in industries (pharmaceuticals, food and personal care products) and agriculture, so as to provide references for the further development and utilization of SOD products in China. Related Articles | Metrics

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Trends and Strategies for Development of Crop Seed Industry in China Xinhai LI, Ming LU, Jun ZHENG, Xiaofeng GU, Lijuan QIU, Yu LI, Youzhi MA, Jianmin WAN Journal of Agricultural Science and Technology    2022, 24 (12): 1-7.   DOI: 10.13304/j.nykjdb.2022.1101 Abstract （1808）   HTML （48）    PDF （506KB）（358）       Knowledge map    Save Seed industry is a strategic and basic industry， and clarifying the development path of China’s crop seed industry is very important to enhance the competitiveness of national agriculture. This paper analyzed the development trend of the international crop seed industry， assessed the opportunities and challenges faced by the development of China’s crop seed industry， and proposed the innovative development strategies of China’s crop seed industry in the new era. Our study reckoned that the new round of scientific and technological revolution characterized by “biotechnology and informatization” had promoted the comprehensive upgrading of the crop seed industry， biotechnology productions had become an important growth point for the development of the seed industry， and multinational seed enterprises had accelerated their occupation of the global seed market. Global scientific and technological innovation and China’s economic transformation and development have brought new opportunities for the development of China’s crop seed industry. Currently，China has basically established a scientific and technological innovation system for crop seed， and seed enterprises have continued to grow， but still facing some challenges such as insufficient original innovation in key science and technology， imperfect seed industry innovation system. Our study suggested that China’s crop seed industry should further strengthen the effective cohesion in the innovation chain and build a deep integration system of production， education and research in the new era， and consolidate the original foundation of seed science and technology， produce high-value intellectual property rights such as key technologies and elite new varieties， as well as cultivate innovative enterprises， improve policy mechanisms and business environment， and promote the high-quality development of the crop seed industry. Reference | Related Articles | Metrics

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Optimization of Extraction Technology and Antifungal Activity of Total Diterpene from Fokienia Hodginsii Shuoshuo FU, Weiyong GONG, Yixuan LIN, Yuanshun LI, Chengcan LIU, Lin NI Journal of Agricultural Science and Technology    2025, 27 (5): 146-155.   DOI: 10.13304/j.nykjdb.2024.0391 Abstract （1734）   HTML （4）    PDF （3472KB）（51）       Knowledge map    Save To optimize the extraction process of total diterpene from Fokienia hodginsii leaves and antifungal activity， the response surface method was used， and the antifungal activity of total diterpenoids of Fokienia hodginsii leaves against 6 plant pathogenic fungi was investigated by mycelium growth rate method. Colletotrichum gloeosporioides Panz. was used as the test bacterium， and the changes of physiological and biochemical indexes of mycelia and the activity of protective enzymes were further determined to explore the antifungal activity of total diterpene extracts. The results showed that the optimal process conditions were as follows： anhydrous ethanol was used as the extraction solvent， extraction temperature was 79 ℃， extraction time was 53 min， liquid-solid ratio was 14.5 mL∶1 g. Under this condition， the total diterpene yield was 36.8%. The total diterpenoids of Fokienia hodginsii leaves had a good inhibitory effect on Colletotrichum gloeosporioides Panz.， and the EC50 value was 1.466 mg·mL-1. The dry weight of mycelia of Colletotrichum gloeosporioides Panz. treated with total diterpenoids was significantly decreased， the conductivity was increased， the content of reducing sugar was significantly increased， and the activities of protective enzymes in the fungi increased first and then decreased. Above results laid a foundation for the resource utilization of Fokienia hodginsii leaves. Table and Figures | Reference | Related Articles | Metrics

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Research Advance on Drought Stress Response Mechanism in Plants WANG Kaiyue, CHEN Fangquan, HUANG Wuxing* Journal of Agricultural Science and Technology    2019, 21 (2): 19-25.   DOI: 10.13304/j.nykjdb.2018.0115 Abstract （1691）      PDF （1029KB）（1642）       Knowledge map    Save Drought stress is one of the major limitations to plant growth and development. Drought can lead to osmotic imbalance, damage of membrane system, decrease of respiration rate and photosynthesis rate, etc. adverse reaction. It not only hinders plant growth and metabolism at different stages, but also affects crops to achieve good quality and high yield. In the interaction between plants and external environment, plants will build drought response, such as changing the structure of plant root system and leaf blades, composition of metabolites and expression of drought-resistant genes to resist drought stress. The paper reviewed the research progress in the mechanism of plant response to drought stress from phenotypic level, physiological level and molecular level. The response of drought stress in phenotypic mainly included the structure change of root system and leaf. The response of drought stress to physiological was mainly reflected in photosynthesis, osmotic regulation, antioxidant metabolism and hormone, etc.. The paper elaborated in detail the molecular mechanism of plant drought stress response and regulatory and functional genes involved in drought stress, discussed the existing problems and look forward the research prospect. Related Articles | Metrics

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Proteome Analysis of Rice Response to Gibberella fujikuroi Infection Lihua SHAO, Peng LI Journal of Agricultural Science and Technology    2025, 27 (6): 126-135.   DOI: 10.13304/j.nykjdb.2024.0337 Abstract （1666）   HTML （0）    PDF （5181KB）（36）       Knowledge map    Save Bakanae disease， a common and highly harmful fungal disease of rice， seriously impacts rice yield. To clarify the up-regulated differential proteins of high-resistance varieties in response to the bakanae pathogen， the TMT （tandem mass tag） quantitative proteomics technology was employed to compare the proteomes before and after infection with the bakanae pathogen， and the significantly differentially expressed proteins with P≤0.05 and a fold change （FC）>1.2 or <0.83 were screened. The results showed that 124 up-regulated and 75 down-regulated differentially expressed proteins were screened. The 124 proteins with up-regulated expression were subjected to gene ontology （GO） enrichment analysis， and got 88 enrichment items， among which 4 items were significantly enriched， including 2 biological processes （response to stress and response to oxidative stress）， 2 molecular functions （peroxidase activity and heme binding）， which should be beneficial to remove peroxides in rice bud tissue and had a protective effect on cells. A total of 45 pathways were obtained by （kyoto encyclopedia of genes and genomes KEGG） enrichment analysis， and 3 pathways were significantly enriched for upregulated differential proteins including phenylpropanoid biosynthesis， the biosynthesis of secondary metabolites and phenylalanine metabolism， respectively， which should be beneficial toimprove disease resistance of rice. There were 3 differential proteins （Os01t0327400-01， Os12t0112000-01， Os01t0963000-04） were common among 4 items of GO significant enrichment and 3 pathways of KEGG significant enrichment， which could be the key proteins in the bakanae disease resistance. Above results provided scientific theoretical basis for the research of disease resistance. Table and Figures | Reference | Related Articles | Metrics

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Studies on Structure and Function of Repressors with EAR Motif ZHANG Jian-fei, QUAN Rui-dang, HUANG Rong-feng    2011, 13 (4): 53-57.   DOI: 10.3969/j.issn.1008-0864.2011.04.08 Abstract （1661）      PDF （454KB）（1416）       Knowledge map    Save Ethylene-responsive element binding factor-associated amphiphilic repression (EAR) motif exists in transcription factor families, such as ERF, C2H2 and AUX/IAA. The EAR motif contains two distinct conservation patterns: L/FDLNL/F(x)P and LxLxL. Through negatively regulating expression of genes involved in development, abiotic and biotic stress responses, plants can keep suitable physiological states under different environmental conditions. This review discusses the evolutionary significance of EAR motif and its patterns, the progress on gene regulation mechanisms and function studies. In addition, combing with our work in laboratory, prospective trends of EAR-related research are predicted. Related Articles | Metrics

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Research Progress of New Crop Germplasm Enhancement in China Hao CHEN, Fei ZHOU, Yongjun LIN Journal of Agricultural Science and Technology    2022, 24 (12): 112-119.   DOI: 10.13304/j.nykjdb.2022.0998 Abstract （1642）   HTML （13）    PDF （493KB）（369）       Knowledge map    Save As a country with a large population and agriculture， our government has always attached great importance to agricultural issues and food security. Based on the national conditions of large population and limited arable land resources， China tolces relying on scientific and technological development to improve the yield level as the main means to ensure the food security. With the active deployment and strong support on agricultural development， China has made continuous breakthroughs and innovations in crop breeding theory and technology in recent years. The genetic dissection of important agronomic traits of crops， the utilization of gene alleles with high breeding value， the innovation and application of gene editing technology， genomic breeding and de novo domestication strategies， industrialization pilot of transgenic soybean and maize provide new ideas and strategies for the creation of new crop germplasm in China. This paper sorted out and reviewed some representative achievements made by China in this field， and briefly summarized and looked forward to the development prospects. Reference | Related Articles | Metrics

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Artificial Intelligence Accelerates Epigenetics and Plant Breeding Weijun GUO, Dongwei LI, Shang XIE, Liwen YANG, Cong LI, Jian TIAN, Li PU, Xiaofeng GU Journal of Agricultural Science and Technology    2022, 24 (12): 90-100.   DOI: 10.13304/j.nykjdb.2022.1031 Abstract （1639）   HTML （34）    PDF （1136KB）（379）       Knowledge map    Save The rapid growth of the global population and tremendous climatic changes are new challenges for food security and compel breeders to develop new avenues of crop breeding. In recent years， the deep integration of biotechnology （BT） with artificial intelligence （AI）， big data， and other information technology （IT） has promoted the rapid development of intelligent design breeding technology. AI-based breeding technology accelerates multi-omics mapping and functional elucidation and the identification of excellent allele variations around the genome， and breeds new varieties through genome design breeding traits. The crucial agricultural breeding traits of crops are synergistically regulated by genetic， epigenetic， and environmental factors that epigenetic marks bridge other factors playing a pivotal role in the regulation of traits. The development of intelligent design technology makes it possible for accurate design breeding based on epigenetic inheritance， which is becoming a new way to improve crop breeding traits. The deep integration of epigenetics and AI has greatly facilitated the identification of genome-wide epigenetic modification sites and the elucidation of regulatory mechanisms. The design of regulatory circuits based on these epigenetic sites has been innovated to achieve the directional creation of new germplasm with excellent comprehensive traits and the cultivation of major varieties. Table and Figures | Reference | Related Articles | Metrics

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Research Progress on the Breeding and Application of Sex Control in Snakehead Mi OU, Jian ZHAO, Qing LUO, Haiyang LIU, Rong HUANG, Yaping WANG, Kunci CHEN Journal of Agricultural Science and Technology    2022, 24 (2): 11-25.   DOI: 10.13304/j.nykjdb.2021.0578 Abstract （1627）   HTML （9）    PDF （3086KB）（302）       Knowledge map    Save Snakehead（Channa）is an important freshwater fish in China，which has obvious sexual dimorphism，with fast growth rate，large size and low feed coefficient ratio in male individuals.Therefore，monosex culture of all-male production is of great economic significance.In this review，the breeding and application of sex control in all-male hybrid snakehead were described.Super-male blotched snakehead（Channa maculata）was produced by sex-specific molecular marker and reproductive endocrine regulation technology，the female parent northern snakehead（Channa argus）was selected for two generations，then “all-male hybrid No.1”（C. argus ♀XX × C. maculata ♂YY）were produced by combining sex control，population selection and hybridization of super-male C. maculata and the selected female C. argus. Growth performance indicated that “all-male hybrid No.1” had high male rate，rapid growth，uniform size，low feed coefficient ratio and wide breeding adaptability.The research on sex control in Channa not only promoted the structural adjustment and industrial upgrading of snakehead，but also provided theoretical and technical support for sex control of other fish，which could accelerate the breeding process of monosex culture. Table and Figures | Reference | Related Articles | Metrics

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2001, 3 (6): 25-29.   Abstract （1623）      PDF （242KB）（3597）       Knowledge map    Save Related Articles | Metrics

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Synergistic Effect of Combination of Antagonists and Chemical Fungicides on Atractylodes macrocephala Blight Disease Jiao WANG, Wenqiao LI, Fengying SHEN, Yaning LI, Daqun LIU Journal of Agricultural Science and Technology    2025, 27 (4): 133-139.   DOI: 10.13304/j.nykjdb.2024.0269 Abstract （1615）   HTML （1）    PDF （1116KB）（100）       Knowledge map    Save In order to investigate the synergistic effect of different antagonistic bacteria， Streptomyces and their combination with chemical fungicides on Atractylodes macrocephala blight disease， the compatibility of different antagonists and chemical fungicides was measured by dilution plate and drug-containing plate method at first. Then the plate confrontation method and growth rate method were used to evaluate the inhibition effect of antagonists compound solution and antagonists+chemical fungicides compound on Phytophthora sansomeana AMPH-1， which was the pathogen of A. macrocephala blight disease. The results showed that the Bacillus BZX-2， BZX-11 and X-119 strains had good compatibility with dimethomorph. The combination of BZX-2+BZX-11+X-119 had the best inhibition effect， and the inhibition rate reached 75.45%， which was lower than that of the treatment using X-119 alone. When the ratio of antagonist combination of Bacillus strains and dimethomorph was 1∶2， the inhibition effect reached 68.61%， which increased 16.83% and 13.59% compared with dimethomorph treatment and antagonists treatment， respectively. By the pot experiment in greenhouse， it was found that the greenhouse control efficiency of BZX-2+BZX-11+X-119 combination with equal proportion mixing was 72.72% on A. macrocephala blight disease， which was 45.45 percentage points higher than that of BZX-2 treatment. Above results provided effective basis for the new strategy of A. macrocephala blight disease control. Table and Figures | Reference | Related Articles | Metrics

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Preliminary Review of Effect on Cotton Production |in China Since Joined into WTO ZHANG Mo-xi, MAO Shu-chun    2005, 7 (3): 52-55.   Abstract （1609）      PDF （235KB）（805）       Knowledge map    Save Since China took part in WTO in November in 2001, the use of cotton increased more than 900 thousand ton per year by the average, the scale of production widened, the total yield enhanced, the price of cotton met the international level with a strong rise and fall, the main product value of cotton and the income of cotton peasanfs both increased for two years and decreased for one year with a big fluctuation range. The importation amount of raw cotton enormously increased with a net import of 2.6 million ton, which made a strong strike on the homemade raw cotton. In the past three years, the total sum of importation of raw cotton, cotton yam and cotton woven fabric were 14,013 million dollars, it was smaller than the total sum of exportation, which was 18,474 million dollars, that means there was a favorable trade balance of 4,234 million dollars. In the chain of cotton industry, there were 4,097 million dollars deficit of raw cotton, 399 million dollars deficit of cotton yarn, and 8,930 million dollars surplus of cotton woven fabric. All these revealed that cotton woven fabric was the most favored one in the chain of cotton industry, cotton yam was the second one, and raw cotton was the least one or the most one suffered from strike. There was nonequilibrium profit in these sub-industries. Related Articles | Metrics

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Research Progress on Cytoplasmic Male Sterility and Fertility Restoration in Gossypium hirsutum L. HOU Si-yu, ZHANG Rui, ZHANG Xiao, GUO San-dui    2010, 12 (1): 1-7.   DOI: 10.3969/j.issn.1008-0864.2010. Abstract （1609）      PDF （674KB）（1105）       Knowledge map    Save Cytoplasmtic male sterile (CMS) has plays an important role in heterosis usage in cotton. CMS and utilization of fertility restorer system have made a breakthrough in conquering breeding bottleneck by artificial emasculation and unfolded a bright outlook for promoting commercial production of hybrid seed. This paper expounds the research and utilization of cytoplasmic male sterility and fertility genetics; the development of molecular markers linked with CMS-related mitochondrial genes and fertility restoring gene; fine mapping of fertility restoring genes and cloning of fertility-related genes. The paper also points out existing problems and prospected researched direction in the future. Related Articles | Metrics

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Transferring Glyphosate-tolerant mG2-epsps Gene into Maize |Inbred Line via Pollen-tube Pathway WANG Xiu-jun, LANG Zhi-hong| LU Wei, LIN Min| SHAN An-shan, HUANG Da-fang    2008, 10 (4): 56-62.   Abstract （1607）      PDF （392KB）（1337）       Knowledge map    Save A plant expression vector pUmG2 harboring the glyphosate-tolerant mG2-epsps gene was constructed and transferred into the maize inbred line X90 by pollen-tube pathway. Among the 1 542 transformed plants of TO generation, 32 glyphosate-tolerant plants were survived after sprayed with 0.25% glyphosate, and 29 of them were con- firmed positive plants by PCR analysis. The average transformation rate was 1.88%. Southern blot and Western blot showed that the rnG2-epsps gene had been introduced into maize genome and expressed correctly. Related Articles | Metrics

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Development Status of Pre-prepared Poultry Meat Dishes Industry， Influencing Factors and Promotion Strategies of Its Quality and Safety Shenghai HUANG, Xiujun TANG, Xiaoxu JIA, Lina MA, Yinyin LIU, Yushi GAO Journal of Agricultural Science and Technology    2024, 26 (6): 133-140.   DOI: 10.13304/j.nykjdb.2023.0543 Abstract （1591）   HTML （1）    PDF （519KB）（707）       Knowledge map    Save Pre-prepared poultry meat dishes plays an important role in improving people’s living standards， rural revitalization， standardization of the catering industry and high-quality development of the poultry industry. However， there are many unfavorable factors affecting the quality and safety during the production and consumption processes of pre-prepared poultry meat dishes. The factors that affect quality and safety in various aspects such as poultry breeding and slaughtering， processing and packaging， storage and transportation， sales and consumption of pre-prepared poultry meat dishes were analyzed. According to the establishment of poultry ecological breeding environment and input management， the construction of poultry slaughtering site and production management， the optimization of pre-prepared poultry meat dishes processing technology and the implementation of green label system， the selection of packaging materials and the application of packaging technology， the establishment of scientific management system and the application of information technology in storage and transportation， the improvement of sales management system and scientific guidance to consumers and so on， corresponding optimization strategies were proposed aiming to promote the improvements of quality and safety and the development of the pre-prepared poultry meat dishes industry， which provided a theoretical basis and model reference for rural revitalization， the solution of the problem of ‘agriculture， rural areas and farmers’， the development of poultry industry and catering industry， the needs of the people for a better life and the construction of ecological civilization. Reference | Related Articles | Metrics

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Formation Mechanism and Control Measures of Continuous Cropping Obstacles in Medicinal Plants ZHANG Zi-long, WANG Wen-quan    2009, 11 (6): 19-23.   Abstract （1584）      PDF （514KB）（1345）       Knowledge map    Save In view of the common and serious situation of continuous cropping obstacles in medicine production, this paper expounded the specific expression of continuous cropping obstacles in medicinal plants; overviewed the recent progress on formation mechanism of continuous cropping obstacles in medicinal plants from three aspects, i.e.  change of soil physical and chemical properties, deterioration of soil biological environment and plant self-allelopathy; and summarized ways to prevent the continuous cropping obstacles in medicinal plants, including rational rotation, soil sterilization, supplementation of nutrient elements or beneficial microorganism and application of comprehensive measures, etc. The paper also discussed hot issues related with continuous cropping obstacles, so as to provide references for further studies. Related Articles | Metrics

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Accelerate the Research and Application of Biological Breeding to Promote the Self-Reliance of Agricultural Science and Technology Wen ZHANG Journal of Agricultural Science and Technology    2022, 24 (12): 8-14.   DOI: 10.13304/j.nykjdb.2022.0733 Abstract （1580）   HTML （13）    PDF （491KB）（997）       Knowledge map    Save Food security is the the pillar of national development and safety. General Secretary XI Jinping pointed that the fundamental solution to stabilize and increase the food production lay in the scientific and technological progress concerning the limited arable land in domestic. At present， a new round of scientific and technological revolution in agriculture characterized by modern biotechnology is breeding major breakthroughs， and plays a leading role in the development of modern agriculture. The development of biological breeding is an important strategy to ensure the national food security and enhance the international competitiveness. This paper introduced the development trend of biological breeding in the development of international agricultural science and technology. For promoting the industrial application of biological breeding， 4 of relationships should be correctly handled， and 4 tasks should be put forward， which provided theoretical guidance for accelerating the development and application of biological breeding technologies in China. Reference | Related Articles | Metrics

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Over-expression of AT-hook Gene AHL27 Can Delay the Flowering of Arabidopsis XIAO Chao-wen,CHEN Fu-lu,FU Yong-fu    2009, 11 (4): 89-94.   Abstract （1567）      PDF （466KB）（1013）       Knowledge map    Save There are 29 AHL proteins (AT-hook motif nuclear localized proteins) in Arabidopsis, but the functions of their mojorities remain unknown. The AHL27 protein, reported in this study, contains an AT-hook motif and a PPC domain. The analysis of mRNA expression in different tissue organs and GUS histochemical staining showed that AHL27 was mainly expressed in roots and flowers. The sub-cellular localization of GFP-AHL27 indicated that AHL27 was a nuclear protein. AHL27 over-expression could inhibit the expression of flowering gene FT and promote the FLC expression, therefore, delay the Arabidopsis flowering time both under long daylight and short daylight conditions. Our data suggested that AHL27 could play a very important role in Arabidopsis growth and development. Related Articles | Metrics

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Advances in Repair Mechanism of |DNA Double-strand Break Damage in Plant ZHU Cai-xia1,2, GU Jia-yu1, SHAO Qun2, WANG Jing1, LIU Lu-xiang1    2010, 12 (5): 17-23.   DOI: 10.3969/j.issn.1008-0864.2010.05.04 Abstract （1567）      PDF （798KB）（994）       Knowledge map    Save The DNA double-strand breaks (DSBs) is one of the most serious form of DNA damage. Non-homologous end-joining (NHEJ) is the major pathway to repair DNA double-strand break damages in both higher plants and animals. The NHEJ pathway does not rely on DNA homology, while the number of such repair factors as Ku protein heterodimer, DNA-PKcs, XRCC4 and ligaseⅣ, will be directly joined the end to repair. The research progresses in major repair pathway and key genes involving in DNA double-strand break damages in plant are summarized. The existing problems and perspectives in this field are also discussed. Related Articles | Metrics

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Epigenetic Regulation and Crop Breeding Xianwei SONG, Shanjie TANG, Xiaofeng CAO Journal of Agricultural Science and Technology    2022, 24 (12): 33-38.   DOI: 10.13304/j.nykjdb.2022.1040 Abstract （1551）   HTML （20）    PDF （440KB）（288）       Knowledge map    Save Epigenetic is a biological phenomenon that DNA sequence unchanged but gene expression was genetically altered， and it includs DNA methylation， histone modification and variants， RNA modification， chromatin remodeling and non coding RNA etc. Epigenetic mainly regulates gene expression and transposon activity at transcriptional and post transcriptional levels. More and more research showed that epigenetic plays an important role in the formation of important agronomic traits and ploidy breeding of crops. This paper reviewed the important progress in the research of epigenetic regulation directly related to crop breeding， and analyzed its potential and ways of application in crop breeding， which were expected to accelerate and promote the basic and applied research of plant epigenetic regulation. Reference | Related Articles | Metrics

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Amino Acid Content Analysis of Different Fresh Corn Varieties at Suitable Harvest Time Baishan LU, Hui DONG, Jiuran ZHAO, Li XU, Yanli FAN, Yaxing SHI, Ronghuan WANG Journal of Agricultural Science and Technology    2023, 25 (11): 132-142.   DOI: 10.13304/j.nykjdb.2023.0680 Abstract （1548）   HTML （8）    PDF （544KB）（825）       Knowledge map    Save The amino acid content of 26 different fresh corn varieties was analyzed by high performance liquid chromatography （HPLC）， and the similarity between different types of fresh corn and WHO/FAO standard amino acid model value was compared， and the amino acid content was analyzed by amino acid ratio coefficient method. The results showed that，the total amino acid content of the tested fresh corn varieties ranged from 6.85 to 16.66 mg·g-1， and Jingketian 533 （16.66 mg·g-1）， Jingkenuo 623 （12.01 mg·g-1） and Nongkenuo 336 （14.44 mg·g-1） was higher than other varieties of sweet， waxy， sweet+waxy type， respectively. Essential amino acids accounted for 10.38%~22.43%， and Jingketian 816 （17.39%）， Jingkenuo 656 （22.43%） and Nongkeyu 368 （18.16%） was higher than other varieties of sweet， waxy， sweet+waxy type， respectively. Analysis of flavor amino acids showed the content of fresh and sweet amino acids of Jingketian 533 and Nongkenuo 336 were higher than that of other varieties. The content of methionine+cysteine was higher than that of WHO/FAO standard mode， while the content of other amino acids were lower than the standard amino acid model value， belonging to the relative lack of amino acids. There were 6 varieties with amino acid ratio coefficient score （SRC） more than 50， including sweet corn varieties Jingketian 183 （58.17）， Jingketian 608 （57.79）， Jingketian 533 （53.32） and Jingketian 816 （53.03）， sweet+waxy corn varieties Nongkeyu 368（51.05） and Nongkenuo 336 （50.63）， indicating that their protein nutritional value was balanced and easy to be absorbed and utilized by the human body. Table and Figures | Reference | Related Articles | Metrics

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Breeding Reports of New Soybean Variety Mengdou 30 ZHANG Wan-hai, SUN Bing-cheng, HU Xing-guo, ZHANG Qi, CHAI Shen    2009, 11 (S2): 133-134.   Abstract （1534）      PDF （121KB）（679）       Knowledge map    Save New soybean variety Mengdou 30 was bred by HunLunbeier institute of agricultural sciences in 1997. It was bred through pedigree method, its female parent is Mengdou 16 and male parent is 89-9. Related Articles | Metrics

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Progress in the Studies and Application of Bacillus thuringiensis GUAN Xiong    2006, 8 (6): 5-11.   Abstract （1534）      PDF （521KB）（2603）       Knowledge map    Save The paper reviewed the development, natural diversity of strains, extensive culture collection, molecular biology, environmental aspects-safety and ecological development, methods of mass production using higher technology. The further improvement is also discussed. Related Articles | Metrics

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Maize Germplasm Pedigree ——|the Division and Discussion of Wu 105 |and Yuanwu 02 Group ZHANG Xue-xin, WANG Jun-bang, XU Shu-xing, WANG Chun-yu, DENG Yong-lin, WANG Min    2009, 11 (S2): 50-55.   Abstract （1519）      PDF （726KB）（827）       Knowledge map    Save Based on a wide range of germplasm collection, accumulation and organization research on maize inbred lines from 1957 to 2004, Wu 105 and Yuanwu 02 group were classified into the second category of ten populations of maize pedigree. Wu 105 was successfuliy applied in top-cross and double cross hybrids, three-way cross, early-maturing single cross hybrids, subsequently bred Shaanxi 1, Huangbai 1, Shaandan 7 and so on. Later,  Wu 105 gave rise to the Wu 109, Tian 4 and other improvement inbred lines, making up some major promotion hybrids such as Shandanhao, Hudanhao, etc.  Shandong Academy of Agricultural Sciences bred Yuanwu 02 by radiation treatment, then bred follow-up derived lines such as Yuanqihao, Luyuan 133, Luyuan 92, forming a new derivative branches, to be widely used in our country. The inbred lines derived from the follow-up  new and improved varieties occupied a large proportion in Jiangsu province, and also be applied in Jilin Tonghua and Fujian.  Wu 105 germplasm expansioned from west to east, from south to north, enriched maize germplasm gene pool of our country. Related Articles | Metrics

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Genetic Regulation Mechanisms of Important Traits and Molecular Design Breeding in Livestock and Poultry Wenyue WANG, Xiaoyu MI, Kangtai SUN, Yichao DAI, Zhipeng YAO, Yuanpeng GAO, Jun LIU, Yiqiang GE, Songmei ZHANG, Xiaoming DENG, Yong ZHANG Journal of Agricultural Science and Technology    2022, 24 (12): 39-47.   DOI: 10.13304/j.nykjdb.2022.0979 Abstract （1501）   HTML （10）    PDF （519KB）（314）       Knowledge map    Save Research on the genetic basis of important economic traits in livestock and poultry is important for the next generation of molecular design breeding， which is the research frontier of scientific and technological innovation in the international livestock and poultry seed industry. Livestock and poultry breeds are rich in resources， diverse in production types， wide in ecological adaptation range， and outstanding in excellent traits in China. However， there are problems of insufficient protection and collection of germplasm resources， unclear analysis of trait characteristics and genetic mechanisms etc.， resulting in insufficient development and utilization of rich livestock and poultry genetic resources in China， and it is difficult to support the research and development and application of molecular design breeding technology. To face the demand of future agriculture modernization and production of livestock and poultry seed industry， this paper summarized the research progress on genetic regulation mechanism and molecular design breeding of important traits of livestock and poultry at home and abroad， as well as the bottlenecks faced by molecular design breeding of livestock and poultry in China， and discussed the development trend of molecular design breeding of livestock and poultry in the future. The review provided significant insights for technological innovation and development of the future livestock and poultry breeding industry. Reference | Related Articles | Metrics

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Research Progress on Rice Ragged Stunt Virus （RRSV） ZHENG Lu-ping, XIE Li-yan| LIAN Ling-li| XIE Lian-hui    2008, 10 (5): 8-12.   Abstract （1500）      PDF （357KB）（1945）       Knowledge map    Save Rice ragged stunt virus （RRSV） is a member of genus Oryzavirus in family Reoviridae. Rice ragged stunt disease caused by RRSV is a serious rice disease in some countries of Southeast Asia, East Asia and South Asia. The occurrence of this disease seriously affects the local rice production. Recent research on RRSV biology and molecular biology were reviewed, and control measures for this disease were proposed. Related Articles | Metrics

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Review of China Agricultural Science and Technology Tu De-yu, Dong Hong-min, Ding Wei-min, Shang Bin    2007, 9 (1): 59-63.   Abstract （1497）      PDF （301KB）（1448）       Knowledge map    Save The proximate, chemical components and ultimate analyses on swine, cattle, chicken and goat manure were conduded to demonstrate the feasibility of energy utilization by thermo-chemJcal technologies. The experimental results state that animal wastes is a type of biomass with comparable content of volatile matter and heat value with some agricultural residues such as corn stalks and rice straws and is adaptable to thermo-chemical managements. The energy balance analyses of air gasification of four kinds of manure were carried out and got the energy conversion efficiency about 50%. By analyzing the net energy production, the arlicle drew the conclusion that thermo-chemical conversion technology is quite effective in energy production in the condition that flesh manure could be dried by weather or composting processes. Animal manure utilization by thermo-chemical conversion technology has environmenta l and economical benefits. Related Articles | Metrics