Effect of Coenzyme Q10 Protein and Taurine Replacing Fish Meal on Growth and Anti-stress Ability of Litopenaeus vannamei

doi:10.13304/j.nykjdb.2025.0574

Abstract

Abstract:

To study the effects of dietary supplementation with coenzyme Q10 （CoQ10） protein and taurine replacing on growth performance and anti-stress resistance in Litopenaeus vannamei， a 56 d breeding experiment was carried out. The experiment set 7 treatments， including fish meal feed as the control （CK）， 5% CoQ10 protein （Q1）， 5% CoQ10 protein+0.3% taurine （Q2）， 5% CoQ10 protein+0.6% taurine （Q3）， 10% CoQ10 protein （Q4）， 10% CoQ10 protein+0.3% taurine （Q5） and 10% CoQ10 protein+0.6% taurine （Q6）. The results showed that the specific growth rate of Q3 treatment was significantly higher than those of other treatment， and its feed coefficient was significantly lower. After transport stress， the survival rate of CoQ10 protein treatments was higher than that of CK， but no significant differences were observed among treatments， and the treatments supplemented taurine exhibited higher plasma superoxide dismutase activity and total antioxidant capacity compared to CK， while malondialdehyde content was significantly lower. In conclusion， under this experimental conditions， substituting fish meal with 5% CoQ10 protein and 0.6% taurine in the diet could improve the growth performance and stress resistance of L. vannamei. Above results revealed the potential of coenzyme Q10 protein and taurine in enhancing the efficiency and sustainable development of shrimp farming， which provided scientific basis for the development of low-fish meal feed for L. vannamei.

Key words: coenzyme Q10 protein, taurine, Litopenaeus vannamei, growth, anti-stress ability

摘要：

为探讨在凡纳滨对虾（Litopenaeus vannamei）饲料中添加辅酶Q10蛋白替代鱼粉并补充牛磺酸对其生长及抗应激能力的影响，开展为期56 d的养殖试验。以鱼粉饲料为对照（CK），分别设置5%辅酶Q10蛋白（Q1）、5%辅酶Q10蛋白+0.3%牛磺酸（Q2）、5%辅酶Q10蛋白+0.6%牛磺酸（Q3）、10%辅酶Q10蛋白（Q4）、10%辅酶Q10蛋白+0.3%牛磺酸（Q5）及10%辅酶Q10蛋白+0.6%牛磺酸（Q6），共计7个处理。结果表明，Q3处理特定生长率高于其他处理，且饵料系数显著低于其他处理；辅酶Q10蛋白处理运输应激后的成活率均高于CK，但处理之间差异不显著；运输应激后，添加牛磺酸处理血浆的超氧化物歧化酶活性、总抗氧化能力高于CK，而丙二醛含量低于CK，但差异不显著。综上所述，在该研究条件下，在饲料中添加5%辅酶Q10蛋白和0.6%牛磺酸替代鱼粉，可以提高凡纳滨对虾的生长性能及抗应激能力。研究结果揭示了辅酶Q10蛋白和牛磺酸在提高对虾养殖效益和可持续发展中的潜力，为凡纳滨对虾低鱼粉饲料的开发提供了科学依据。

关键词: 辅酶Q10蛋白, 牛磺酸, 凡纳滨对虾, 生长, 抗应激能力

CLC Number:

S963

Liangxing ZHOU, Min ZHAO, Bin SHU, Kunlan WU, Qingkai XI, Xuanyu REN, Ying CHEN, Jiangao LIU. Effect of Coenzyme Q10 Protein and Taurine Replacing Fish Meal on Growth and Anti-stress Ability of Litopenaeus vannamei[J]. Journal of Agricultural Science and Technology, 2025, 27(11): 56-63.

周良星, 赵敏, 舒斌, 吴坤岚, 席庆凯, 任宣宇, 陈颖, 刘建高. 辅酶Q10蛋白和牛磺酸替代鱼粉对凡纳滨对虾生长及抗应激能力的影响[J]. 中国农业科技导报, 2025, 27(11): 56-63.

Figures/Tables 5

References 22

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类型Type	指标Index	CK	Q1	Q2	Q3	Q4	Q5	Q6
配方 Ingredient	鱼粉Fish meal	29.00	23.50	23.50	23.50	18.00	18.00	18.00
	辅酶Q10蛋白CoQ10 protein	0.00	5.00	5.00	5.00	10.00	10.00	10.00
	牛磺酸Taurine	0.00	0.00	0.30	0.60	0.00	0.30	0.60
	虾粉Shrimp meal	6.00	6.00	6.00	6.00	6.00	6.00	6.00
	鸡肉粉Poultry powder	8.50	8.50	8.50	8.50	8.50	8.50	8.50
	虾膏Shrimp paste	1.50	1.50	1.50	1.50	1.50	1.50	1.50
	鱼溶浆Fish soluble	5.00	5.00	5.00	5.00	5.00	5.00	5.00
	酵母水解物Yeast hydrolyte	2.50	2.50	2.50	2.50	2.50	2.50	2.50
	花生饼Peanut cake	8.00	8.00	8.00	8.00	8.00	8.00	8.00
	豆粕Soybean meal	11.85	11.85	11.85	11.85	11.85	11.85	11.85
	高筋面粉Gluten flour	20.50	20.50	20.50	20.50	20.50	20.50	20.50
	大豆磷脂油Soybean-lecithin oil	3.00	3.25	3.25	3.25	3.50	3.50	3.50
	膨润土Bentonite	0.35	0.60	0.30	0.00	0.85	0.55	0.25
	磷酸二氢钙Ca（H₂PO₄）₂	1.80	1.80	1.80	1.80	1.80	1.80	1.80
	预混料Premix	2.00	2.00	2.00	2.00	2.00	2.00	2.00
营养成分Nutritional ingredient	粗蛋白Crude protein	45.87	45.34	45.57	45.62	45.68	45.73	45.71
	粗脂肪Crude lipid	7.83	7.89	7.92	7.91	7.75	7.85	7.73
	粗灰分Crude ash	11.61	11.54	11.48	11.71	11.34	11.27	11.42
	钙Ga	2.18	2.17	1.85	1.99	1.71	1.87	1.89
	磷P	1.82	1.79	1.62	1.77	1.8	1.72	1.72
	盐分NaCl	0.89	0.48	0.51	1.14	0.97	1.12	1.09
	粗纤维Crude fiber	1.84	1.31	2.13	2.72	9.44	8.66	7.55
	氨基酸总和Total amino acids	38.94	39.44	40.08	38.99	40.06	39.25	39.54
	赖氨酸Lys	2.80	2.64	2.70	2.59	2.49	2.50	2.54
	蛋氨酸Met	0.92	0.92	0.93	0.88	0.95	0.93	0.94
	苏氨酸Thr	1.71	1.73	1.76	1.71	1.78	1.73	1.73
	缬氨酸Val	1.79	1.81	1.93	1.83	1.89	1.74	1.75
	亮氨酸Leu	3.21	3.27	3.34	3.26	3.38	3.30	3.30
	异亮氨酸Ile	1.40	1.37	1.49	1.41	1.42	1.30	1.31
	苯丙氨酸 Phe	1.96	2.02	2.04	2.01	2.08	2.07	2.05
	精氨酸Arg	2.75	2.81	2.87	2.80	2.91	2.81	2.84
	组氨酸His	1.23	1.23	1.24	1.21	1.25	1.23	1.23
	天冬氨酸Asp	4.22	4.25	4.29	4.18	4.35	4.30	4.32
	胱氨酸Cys	0.48	0.49	0.49	0.48	0.48	0.47	0.49
	丙氨酸Ala	2.64	2.77	2.78	2.73	2.89	2.87	2.88
营养成分Nutritional ingredient	甘氨酸Gly	2.79	2.91	2.94	2.88	2.92	2.88	2.90
	谷氨酸Glu	6.87	6.90	6.94	6.80	6.90	6.82	6.85
	脯氨酸Pro	2.15	2.26	2.29	2.20	2.30	2.25	2.34
	丝氨酸Ser	2.02	2.06	2.05	2.02	2.07	2.05	2.07

类型Type	指标Index	CK	Q1	Q2	Q3	Q4	Q5	Q6
配方 Ingredient	鱼粉Fish meal	29.00	23.50	23.50	23.50	18.00	18.00	18.00
	辅酶Q10蛋白CoQ10 protein	0.00	5.00	5.00	5.00	10.00	10.00	10.00
	牛磺酸Taurine	0.00	0.00	0.30	0.60	0.00	0.30	0.60
	虾粉Shrimp meal	6.00	6.00	6.00	6.00	6.00	6.00	6.00
	鸡肉粉Poultry powder	8.50	8.50	8.50	8.50	8.50	8.50	8.50
	虾膏Shrimp paste	1.50	1.50	1.50	1.50	1.50	1.50	1.50
	鱼溶浆Fish soluble	5.00	5.00	5.00	5.00	5.00	5.00	5.00
	酵母水解物Yeast hydrolyte	2.50	2.50	2.50	2.50	2.50	2.50	2.50
	花生饼Peanut cake	8.00	8.00	8.00	8.00	8.00	8.00	8.00
	豆粕Soybean meal	11.85	11.85	11.85	11.85	11.85	11.85	11.85
	高筋面粉Gluten flour	20.50	20.50	20.50	20.50	20.50	20.50	20.50
	大豆磷脂油Soybean-lecithin oil	3.00	3.25	3.25	3.25	3.50	3.50	3.50
	膨润土Bentonite	0.35	0.60	0.30	0.00	0.85	0.55	0.25
	磷酸二氢钙Ca（H₂PO₄）₂	1.80	1.80	1.80	1.80	1.80	1.80	1.80
	预混料Premix	2.00	2.00	2.00	2.00	2.00	2.00	2.00
营养成分Nutritional ingredient	粗蛋白Crude protein	45.87	45.34	45.57	45.62	45.68	45.73	45.71
	粗脂肪Crude lipid	7.83	7.89	7.92	7.91	7.75	7.85	7.73
	粗灰分Crude ash	11.61	11.54	11.48	11.71	11.34	11.27	11.42
	钙Ga	2.18	2.17	1.85	1.99	1.71	1.87	1.89
	磷P	1.82	1.79	1.62	1.77	1.8	1.72	1.72
	盐分NaCl	0.89	0.48	0.51	1.14	0.97	1.12	1.09
	粗纤维Crude fiber	1.84	1.31	2.13	2.72	9.44	8.66	7.55
	氨基酸总和Total amino acids	38.94	39.44	40.08	38.99	40.06	39.25	39.54
	赖氨酸Lys	2.80	2.64	2.70	2.59	2.49	2.50	2.54
	蛋氨酸Met	0.92	0.92	0.93	0.88	0.95	0.93	0.94
	苏氨酸Thr	1.71	1.73	1.76	1.71	1.78	1.73	1.73
	缬氨酸Val	1.79	1.81	1.93	1.83	1.89	1.74	1.75
	亮氨酸Leu	3.21	3.27	3.34	3.26	3.38	3.30	3.30
	异亮氨酸Ile	1.40	1.37	1.49	1.41	1.42	1.30	1.31
	苯丙氨酸 Phe	1.96	2.02	2.04	2.01	2.08	2.07	2.05
	精氨酸Arg	2.75	2.81	2.87	2.80	2.91	2.81	2.84
	组氨酸His	1.23	1.23	1.24	1.21	1.25	1.23	1.23
	天冬氨酸Asp	4.22	4.25	4.29	4.18	4.35	4.30	4.32
	胱氨酸Cys	0.48	0.49	0.49	0.48	0.48	0.47	0.49
	丙氨酸Ala	2.64	2.77	2.78	2.73	2.89	2.87	2.88
营养成分Nutritional ingredient	甘氨酸Gly	2.79	2.91	2.94	2.88	2.92	2.88	2.90
	谷氨酸Glu	6.87	6.90	6.94	6.80	6.90	6.82	6.85
	脯氨酸Pro	2.15	2.26	2.29	2.20	2.30	2.25	2.34
	丝氨酸Ser	2.02	2.06	2.05	2.02	2.07	2.05	2.07

处理 Treatment	初均质量 Initial average weight/g	末均质量 Final average weight/g	摄食率 Feeding rate/%	增重率 Weight gain rate/%	特定生长率 Specific growth rate/（%·d^-1）	饵料系数 Feed coefficient
CK	1.23±0.09	8.11±0.27 b	3.98±0.04 b	494.77±44.83 bc	3.30±0.14 bc	1.51±0.05 b
Q1	1.23±0.08	7.33±0.69 ab	4.33±0.21 c	437.77±28.95 ab	3.11±0.10 ab	1.71±0.08 cd
Q2	1.25±0.07	7.15±0.72 ab	4.14±0.22 bc	438.74±35.33 ab	3.12±0.12 ab	1.63±0.11 bcd
Q3	1.27±0.05	8.26±0.50 b	3.63±0.12 a	525.79±53.99 c	3.39±0.16 c	1.35±0.08 a
Q4	1.32±0.09	7.97±0.74 ab	3.98±0.22 b	436.03±14.45 ab	3.11±0.05 ab	1.57±0.08 bc
Q5	1.24±0.08	6.91±0.40 a	4.39±0.09 c	414.24±20.56 a	3.03±0.07 a	1.76±0.02 d
Q6	1.30±0.08	7.18±0.86 ab	4.12±0.22 bc	419.43±51.03 a	3.04±0.18 a	1.65±0.14 bcd

处理 Treatment	初均质量 Initial average weight/g	末均质量 Final average weight/g	摄食率 Feeding rate/%	增重率 Weight gain rate/%	特定生长率 Specific growth rate/（%·d^-1）	饵料系数 Feed coefficient
CK	1.23±0.09	8.11±0.27 b	3.98±0.04 b	494.77±44.83 bc	3.30±0.14 bc	1.51±0.05 b
Q1	1.23±0.08	7.33±0.69 ab	4.33±0.21 c	437.77±28.95 ab	3.11±0.10 ab	1.71±0.08 cd
Q2	1.25±0.07	7.15±0.72 ab	4.14±0.22 bc	438.74±35.33 ab	3.12±0.12 ab	1.63±0.11 bcd
Q3	1.27±0.05	8.26±0.50 b	3.63±0.12 a	525.79±53.99 c	3.39±0.16 c	1.35±0.08 a
Q4	1.32±0.09	7.97±0.74 ab	3.98±0.22 b	436.03±14.45 ab	3.11±0.05 ab	1.57±0.08 bc
Q5	1.24±0.08	6.91±0.40 a	4.39±0.09 c	414.24±20.56 a	3.03±0.07 a	1.76±0.02 d
Q6	1.30±0.08	7.18±0.86 ab	4.12±0.22 bc	419.43±51.03 a	3.04±0.18 a	1.65±0.14 bcd

处理 Treatment	运输应激前成活率 Survival rate before transport stress	运输应激后成活率 Survival rate after transport stress
CK	0.90±0.04	0.63±0.08
Q1	0.90±0.05	0.68±0.10
Q2	0.94±0.04	0.72±0.08
Q3	0.96±0.01	0.73±0.14
Q4	0.89±0.11	0.70±0.09
Q5	0.93±0.08	0.73±0.12
Q6	0.94±0.07	0.75±0.15