Abstract: Growth regulation factor (GRF) is a type of special transcription factor in plants, which plays an important role in plant hormone regulation, growth and development, stress response. This paper screened and identified the GRF gene family members of sorghum, obtained 8 GRF genes, and then analyzed their conservative protein domain and gene structure, function promoter elements, evolutionary relationship and collinearity. The results showed 8 GRF genes were conserved in structure in sorghum. The analysis of function promoter elements revealed these genes had drought, low temperature and jasmonate response elements etc, indicating these genes participate in the response to biotic and abiotic stress. A candidate gene (SORBI_3002G297800) for salt tolerance and a gene (SORBI_3006G203400) for hormone stress  were screened through transcriptome expression analysis of salt stress and uniconazole treatment. The results provided important reference for further studing the function of these genes in sorghum.

Key words: sorghum, GRF, authenticate, expression analysis, abiotic stress

摘要： 生长调控因子（growth regulation factor，GRF）是植物中特有的一类转录因子，在植物激素调控、生长发育以及胁迫应答等多个领域扮演重要角色。对高粱GRF基因家族成员进行筛选鉴定，得到8个家族基因，分析了这些基因的理化性质、保守蛋白序列、基因功能及其共线性，分析了这些基因在非生物胁迫下转录组表达差异。结果表明，GRF基因家族结构较为保守，启动子元件含有干旱、低温、茉莉酸等响应元件，推测该家族基因参与生物及非生物胁迫应答响应。通过盐胁迫和烯效唑处理下的转录组数据分析，得到一个与耐盐相关的基因（SORBI_3002G297800）和一个与激素相关的基因（SORBI_3006G203400），为进一步探究这些基因在高粱应对非生物胁迫中的作用提供了重要参考。

关键词: 高粱, GRF, 鉴定, 表达分析, 非生物胁迫