关键词: 近等基因系, SNP芯片, 全基因组关联分析, 穗轴颜色, 分子标记

Abstract: Maize (Zea mays L.) is an important food crop, and also used as an ideal material for plant genetics and functional genome research. The near-isogenic lines (NILs) are important materials for the construction of molecular genetic maps, quantitative trait locus (QTL) and molecular markers-assisted breeding. Maize hybrids Zhengdan 958 (Zheng 58 × Chang 7-2) and Xianyu 335 (PH6WC × PH4CV) are widely cultivated in China, showing differences in plant architecture, ear traits, grain moisture and dehydration rate, etc. Here, Zheng 58 NILs and Chang 7-2 NILs were conducted by introducing into PH6WC and PH4CV background. These NILs were obtained by consecutively selective backcrossing and selfing, and then genotypes of these NILs were detected using maize 55 K SNP array. These NILs showed different agronomic traits including plant architecture, ear traits, grain water content and dehydration rate. And P1 locus was identified through genome-wide association study (GWAS) analysis of cob glume color on these NILs. In the future, the NILs  combined with GWAS could be used to identify genetic factors  which determine plant height, ear height, grain filling rate (GFR) and grain dehydration rate (GDR). And SNP molecular markers would be developed for molecular marker-assisted selection breeding to improve breeding efficiency.

Key words: near-isogenic lines, SNP array, genome-wide association study, cob color, molecular marker