关键词: 猕猴桃, 叶片, 叶柄, 生根培养, 愈伤组织

Abstract: In order to achieve factory seedling of Actinidia chinensis ‘YangshiJinhong 50’ by tissue culture, the leaves and petioles of tissue culture seedlings of Actinidia chinensis ‘YangshiJinhong 50’ were used as explants to directly induce adventitious buds. The effects of different plant growth regulators on adventitious bud induction and rooting induction were studied and efficient methods of in vitro regeneration were screened. The results show that the optimal medium for direct induction of adventitious buds was MS+2 mg/L 6-BA+0.2 mg/L NAA. The average browning rate was 7.5%, and the average germination rate was 92.5%. The optimal medium for directly inducing adventitious buds from petiole was MS+2 mg/L 6-BA+0.3 mg/L NAA, and the average germination rate was 89.17%. The best medium for rooting culture was 1/2 MS+0.9 mg/L IBA. The roots were shoved in the medium. The average size of callus was 1.03 cm. The average rooting rate was 100%, and the average root length was 3.19 cm. The average number of roots was 12.58, and the optimum time for hardening-seedling was 7 d.

Key words: Actinidia chinensis, leaf, petiole, rooting culture, callus