关键词: 飞蝗, 昆虫储存蛋白, 基因表达, 实时荧光定量PCR, 免疫

Abstract: Hexamerin is a key functional protein and plays an important role in the growth and development of insects and the immune response. To further analyze the immune function of the hexamerin gene family in Locusta migratoria， qPCR method was adopted to quantitatively analyze the expression of 4 members (L.m.Hx1，L.m.Hx2, L.m.Hx3 and L.m.Hx4) of the hexamerin gene family in different tissues of the adult stages in this paper, and the cuticle and fat body with higher expression levels were selected as materials to conduct E.coli stimulation experiments on adult Locusta migratoria. The results showed that after the bacteria stimulated, the relative expression levels of L.m.Hx1 and L.m.Hx2 showed trends from declining to rising, while the relative expression levels of L.m.Hx3 and L.m.Hx4 showed trends from rising to declining, which indicated that the genes of the hexamerins family had immune function. L.m.Hx1 and L.m.Hx2 showed negative regulation of immune response, L.m.Hx3 and L.m.Hx4 showed positive regulation of immune response, and hexamerin gene family members manifest functional compensation. Above results provided basis for further studying the immune function and mechanism of hexamerin gene family in Locusta migratoria, as well as reference for pest control.

Key words: Locusta migratoria, hexamerin, gene expression, qPCR, immunity