Abstract:

The YUCCA gene encodes flavin monooxygenase， which plays an important regulatory role in plant growth and development. In order to explore the effect of YUCCA gene on the growth and development of Huperzia serrata， bioinformatics and tissue-specific expression analysis of YUCCA gene family of H. serrata were carried out. The results showed that 18 members of the HsYUCCA gene family were identified in the full-length transcriptome of H. serrata. The amino acid length was 150~544 aa， the relative molecular weight of the protein was 16 105.53~59 058.24 Da， and the isoelectric point was 4.36~9.63. Among them， there were 9 basic proteins， 4 stable proteins， 15 hydrophilic proteins， and 7 proteins with transmembrane structure. The subcellular localization ofHsYUCCA were in the nucleus， cell membrane， cytoplasm， mitochondria， chloroplast and peroxisome. The expression of HsYUCCA4 was the highest in spores， stems and spores， and the expression of HsYUCCA6 was the highest in leaves. HsYUCCA had 35 cis-acting elements such as hormone response， light response and stress response， and had 15 conserved motifs. The physical and chemical properties， subcellular localization， secondary structure， tertiary structure and conserved motifs of HsYUCCA1， HsYUCCA2 and HsYUCCA4， HsYUCCA5 and HsYUCCA6， HsYUCCA7 and HsYUCCA13 were similar， indicating that their biological functions were similar， and the phylogenetic development showed certain homology， and the evolutionary process tended to be consistent. Above results provided a theoretical basis for the future analysis of the possible mechanism of YUCCA gene in the growth， development and reproduction of H. serrata.

Key words: Huperzia serrata, YUCCA gene family, bioinformatics, expression analysis

摘要：

YUCCA 基因编码黄素单加氧酶，在植物生长发育过程中起重要调控作用。为探究YUCCA 基因对蛇足石杉生长发育的作用，对蛇足石杉YUCCA 基因家族进行生物信息学及组织特异性表达分析。结果表明，在蛇足石杉全长转录组中共鉴定出 18个 HsYUCCA 基因家族成员，氨基酸长度为150~544 aa，蛋白质相对分子量为16 105.53~59 058.24 Da，等电点在4.36~9.63，其中，有9个为碱性蛋白，有4个为稳定蛋白，有15个为亲水蛋白，有7个具有跨膜结构，分布在细胞核、细胞膜、细胞质、线粒体、叶绿体以及过氧化物酶体中。HsYUCCA4 在孢子、茎、芽胞中的表达量最高，HsYUCCA6在叶中的表达量最高。HsYUCCA 启动子区拥有激素响应、光响应、胁迫响应等35种顺式作用元件，并具有15 种保守基序。HsYUCCA1、HsYUCCA2和HsYUCCA4，HsYUCCA5和HsYUCCA6，HsYUCCA7和HsYUCCA13具有相似的理化性质、亚细胞定位、二级结构、三级结构及保守基序，系统发育表现出一定的同源性，进化过程趋于一致。以上研究结果为解析蛇足石杉 YUCCA 基因在生长发育和繁殖过程中的作用机理提供了理论依据。

关键词: 蛇足石杉, YUCCA基因家族, 生物信息学, 表达分析