Abstract: In order to optimize to the extraction process of polysaccharide from Agaricus bisporus, and detect its antioxidant activity in vitro, the effects of extraction time, extraction temperature, and liquid-to-solid ratio on extraction of polysaccharide were investigated by response surface method. Moreover the abilities of eliminating DPPH free radicals and hydroxyl free radicals, total antioxidant activity were measured to investigate the antioxidant activity of A. bisporus polysaccharide in vitro. The results showed that the optimized extraction technological parameters of A. bisporus polysaccharide were 94℃ of extraction temperature, 3 hours of extraction time and 34 mL/g of liquid-to-soild. A. bisporus polysaccharide content extracted under the optimal condition was 5.18%, which was consistent with the predicted value. IC50 of Agaricus bisporus polysaccharide to DPPH free radicals and hydroxyl free radicals were 4.94 mg/mL and 2.77 mg/mL, respectively. Total antioxidant activity increased with the increase of polysaccharide concentration. It was showed that A. bisporus polysaccharide extracted by the optimized method had antioxidative ability in vitro. The results provided reference for comprehensive development and utilization of A. bisporus polysaccharide.

Key words: Agaricus bisporus polysaccharide, response surface methodology, extraction process, antioxidant activity

摘要： 为了研究双孢蘑菇中多糖的提取工艺以及该多糖在体外的抗氧化活性，检测不同提取温度、提取时间和液料比对双孢蘑菇多糖提取率的影响，通过响应面法优化双孢蘑菇多糖的提取工艺，并测定双孢蘑菇多糖清除1,1-二苯基-2-三硝基苯肼（DPPH）自由基和羟基自由基的能力、总抗氧化活性对其体外抗氧化活性进行研究。结果表明，优化的双孢蘑菇多糖提取工艺参数为：提取温度94℃、提取时间3 h、液料比34 mL/g，在此工艺条件下提取得到的双孢蘑菇多糖含量为5.18%，与预测值一致；双孢蘑菇多糖对DPPH自由基和羟基自由基清除的半清除浓度（IC50）分别为4.94 mg/mL和2.77 mg/mL，双孢蘑菇多糖总抗氧化活性随着多糖浓度的增加而逐渐升高，表明双孢蘑菇多糖具有一定的体外抗氧化能力。结果为双孢蘑菇多糖的综合开发利用提供参考依据。

关键词: 双孢蘑菇多糖, 响应面分析, 提取工艺, 抗氧化活性