Phenolic Composition and Antioxidant Activity of Pear Species During Fruit Development Comparison

doi:10.13304/j.nykjdb.2022.0162

Abstract

Abstract:

In order to study the dynamic changes of phenolic substances and antioxidant activities during the development of different pear varieties， the total flavonoids， total phenols， phenolic substances， 1，1-diphenyl-2-picrylhydrazylradical （DPPH） and 2， 2’-azino-bis（3-ethylbenzothiazoline-6-sulfonic acid）（ABTS⁺） free radical scavenging abilityin the fruits of Kuche Amute， Dangshan pear and Ma pear at different developmental stages wereanalyzed and determined. The results showed that the contents of total flavonoids and total phenols in the three kinds of pear fruits were basically the same during the development process， the content was higher in young fruit stage， and gradually decreased with fruit development. A total of 13 phenolic substances were detected in the three types of pear fruits by high performance liquid chromatography， and the content of arbutin was the highest， with an average of 3 353.43 μg·g^-1 DW， accounting for 51.88% of the total components； orthoic acid was the second with an average content of 2 350.29 μg·g^-1 DW， accounting for 36.34% of the total components； the content of other phenolic components was low， distributed between 8.09～150.65 μg·g^-1 DW， only 0.13%～2.33% of the total phenolic content. The antioxidant activity detection showed that the free radical scavenging power of DPPH and ABTS⁺ was Dangshan pear > Kuqa Amute > Ma pear. Correlation analysis showed that the total flavonoids， total phenols， arbutin， catechin， chlorogenic acid， epicatechin and other substances in pear fruit had a very significant positive correlation with DPPH free radical scavenging ability （P<0.01）. The results provided the basis for in-depth research and development of natural antioxidant health drugs and functional foods.

Key words: pear, phenols, antioxidant activity, fruit development

摘要：

为研究不同品种梨果实发育过程中酚类物质和抗氧化活性的动态变化，对不同发育阶段的库车阿木特、砀山酥梨和麻梨果实中的总黄酮、总酚、酚类物质组成以及1，1-二苯基-2-苦肼（DPPH）、2，2’-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS⁺）自由基清除能力进行分析测定。结果表明，3种梨果实中的总黄酮和总酚含量在发育过程中变化基本一致，幼果期含量较高，随果实发育而逐渐降低。高效液相色谱检测发现，3种类型梨果实中共检测到13种酚类物质，均以熊果苷含量最高，平均为3 353.43 μg·g^-1 DW，占总组分的51.88%；其次是绿原酸，平均含量为2 350.29 μg·g^-1 DW，占总组分的36.34%；其他酚类组分含量较低，分布在8.09～150.65 μg·g^-1 DW之间，仅占总酚含量的0.13%～2.33%。抗氧化活性检测发现，DPPH和ABTS⁺自由基清除力依次为砀山酥梨>库车阿木特>麻梨。相关性分析显示，梨果实中总黄酮、总酚、熊果苷、儿茶素、绿原酸、表儿茶素等物质与DPPH自由基清除能力均存在极显著正相关关系（P<0.01）。研究结果为天然抗氧化保健药物以及功能性食品的深度研究与开发提供依据。

关键词: 梨, 多酚物质, 抗氧化活性, 果实发育

CLC Number:

S661.2

Dengyang LU, Xin WANG, Zhanghu TANG, Cuiyun WU, Yunfeng PU, Min YAN, Jingkai BAO, Xi JIANG. Phenolic Composition and Antioxidant Activity of Pear Species During Fruit Development Comparison[J]. Journal of Agricultural Science and Technology, 2023, 25(9): 97-104.

卢登洋, 王鑫, 唐章虎, 吴翠云, 蒲云峰, 闫敏, 鲍荆凯, 姜喜. 梨果实发育过程中酚类物质组成及抗氧化活性比较[J]. 中国农业科技导报, 2023, 25(9): 97-104.

Figures/Tables 6

References 25

1	张小双. 中国北方梨种质荧光标记SSR分子身份证的构建和亲缘关系分析[D].北京:中国农业科学院,2018.
	ZHANG X S. Molecular identification establishment and genetic relationship analysis of pear accessions in northern china using the fluorescent-labeled SSR markers [D]. Beijing: Chinese Academy of Agricultural Sciences, 2018.
2	蒋媛. 两个梨优系的生物学特性研究与分子鉴定[D].阿拉尔:塔里木大学,2015.
	JIANG Y. The research on two good strains of pears by biogical characteristics and molecular identification [D]. Alar: Tarim University, 2015.
3	罗桂环. 梨史源流[J].古今农业,2014,28(3):49-58.
	LUO G H. On the origin and culture of pear history in china [J]. Ancient Mod. Agric.,2014,28(3): 49-58.
4	鲁敏,汤浩茹,罗娅,等. SSR标记技术在梨属植物亲缘关系研究中的应用[J].北方园艺,2012,36(23):193-196.
	LU M, TANG H R, LUO Y, et al.. The applied research progression of SSR in studying parentage relationship of pyrus L. [J]. Northern Hortic.,2012,36(23): 193-196.
5	蒲富慎. 梨种质资源及其研究[J].中国果树,1988,30(2):42-46.
	PU F S. Pear germplasm resources and their research [J]. Chin. Fruits,1988,30(2): 42-46.
6	曾少敏. 梨果实主要酚类物质含量及抗氧化活性研究[D].北京:中国农业科学院,2013.
	ZENG S M. Study on the major phenolic contents and antioxidant activities in pear eruits[D]. Beijing:Chinese Academy of Agricultural Sciences,2013.
7	王杰. 梨历史与产业发展研究[D].福州:福建农林大学,2011.
	WANG J. Culture and industrial development of pear in China [D]. Fuzhou: Fujian Agriculture and Forestry University, 2011.
8	LEE K H, CHO J Y, LEE H J, et al.. Hydroxycinnamoylmalic acids and their methyl esters from pear (Pyrus pyrifolia Nakai) fruit peel [J]. J. Agric. Food Chem.,2011,59(18):10124-10128.
9	KOLNIAK O J. Content of bioactive compounds and antioxidant capacity in skin tissues of pear [J]. J. Funct. Foods, 2016,23:40 -51.
10	LEE K H, CHO J Y, LEE H J. Isolation and identification of phenolic compounds from an Asian pear (Pyrus pyrifolia Nakai) fruit peel [J]. Food Sci. Biotechnol., 2011,20(6): 1539-1545.
11	王鑫, 姜喜, 于军, 等. ‘绿宝石’梨果实发育期酚类物质的动态变化及其抗氧化活性[J]. 塔里木大学学报, 2021, 33 (2):25-31.
	WANG X, JIANG X, YU J, et al.. Dynamics of phenolic substances and their antioxidant activityduring fruit development of ‘Lubaoshi’ pears [J]. J. Tarim Univ., 2021,33 (2): 25-31.
12	姜喜,唐章虎,吴翠云,等. 3种梨品种果实发育过程中酚类物质及其抗氧化能力分析[J].食品科学,2021,42(23):99-105.
	JIANG X, TANG Z H, WU C Y, et al.. Analysis of phenolic substances and antioxidant capacity of three pear fruits during development [J ]. Food Sci.,2021,42(23):99-105.
13	YEON O P, JANG J C, JIN H C,et al.. Antioxidant activities of young and mature fruit in three Asian pear cultivars [J]. Korean J. Hortic. Sci. Technol.,2012, 30(2):208-213.
14	BUCCHINI A, SCOCCIANTI V, RICCI D, et al.. Cocomerina pear: an old and rare fruit with red pulp. Analysis of phenolic content and antioxidant/anti-inflammatory capacity [J/OL]. CyTA-J. Food, 2016, 14(4):1147084 [2022-05-10]. .
15	李雁琴,宋丽军,张丽,等. 不同品种红枣冻干片的理化品质及抗氧化性比较[J].食品研究与开发,2020,41(11):28-33, 65.
	LI Y Q, SONG L J, ZHANG L, et al.. Comparison of physic-chemistry qualities and antioxidant activity of frozen slices of different jujube varieties [J]. Food Res. Dev., 2020,41 (11): 28-33, 65.
16	PU Y F, DIGN T, WANG W J, et al.. Effect of harvest, drying and storage on the bitterness, moisture, sugars, free amino acids and phenolic compounds of jujube fruit (Zizyphus jujuba cv. Junzao) [J]. J. Sci. Food Agric.,2018, 98(2):628-634.
17	刘杰超,张春岭,陈大磊,等. 不同品种枣果实发育过程中多酚类物质、Vc含量的变化及其抗氧化活性[J].食品科学,2015,36(17):94-98.
	LIU J C, ZHANG C L, CHEN D L, et al.. Changes in phenolics, vitamin C and antioxidant capacity during development of different cultivars of jujube fruits [J]. Food Sci.,2015,36 (17): 94-98.
18	曾少敏,杨健,王龙,等. 梨果实酚类物质含量及抗氧化能力[J].果树学报,2014,31(1):39-44.
	ZENG S M, YANG J, WANG L,et al.. Study on phenolics content and its antioxidant activity in the fruits of pear species [J]. J. Fruit Sci., 2014,31 (1): 39-44.
19	田兰兰,刘婧琳,郑战伟,等. 苹果多酚组分及其生理功能研究进展[J].食品工业科技,2011,32(12):552-557.
	TIAN L L, LIU J L, ZHENG Z W,et al.. Research progress of compounds and physiological functions of apple polyphenol [J]. Sci. Technol. Food Ind., 2011,32 (12): 552-557.
20	赵梅,张绍铃,齐开杰,等. 梨幼果多酚提取工艺优化及其成分分析[J].食品工业科技,2013,34(6):268-271.
	ZHAO M, ZHANG S L, QI K J, et al..Optimization of extraction of polyphenols from pear fruitlet pulp and analysis of its composition [J]. Sci. Technol. Food Ind., 2013,34 (6): 268-271.
21	黄怡,高春丽,毕阳,等. 低温贮藏期间梨果皮酚类物质及抗氧化性变化[J].食品与发酵工业,2019,45(19):219-226.
	HUANG Y, GAO C L, BI Y, et al.. Changes in phenolic compounds and antioxidant activity of pear peel during cold storage [J]. Food Fermentation Ind., 2019,45 (19): 219-226.
22	邹丽红,张玉星.砂梨果肉褐变与酚类物质及相关酶活性的相关分析[J].果树学报,2012,29(6):1022-1026.
	ZOU L H, ZHANG Y X. Correlation analysis of flesh browning between phenolic compound and relavent enzymatic activity in fruit of Pyrus pyrifolia [J].J. Fruit Sci., 2012,29 (6): 1022-1026.
23	王晓宇,杜国荣,李华. 抗氧化能力的体外测定方法研究进展[J].食品与生物技术学报,2012,31(3):247-252.
	WANG X Y, DU G R, LI H. Progress of analytical methods for antioxidant capacity in vitro [J]. J. Food Sci. Biotechnol., 2012,31 (3): 247-252.
24	张云. 猕猴桃多酚分离鉴定及抗氧化活性研究[D].长沙:中南林业科技大学,2019.
	ZHANG Y. Isolation, identification and antioxidant activity of kiwifruit polyphenols [D]. Changsha: Central South University of Forestry and Technology, 2019.
25	黄春辉,廖光联,谢敏,等. 不同猕猴桃品种果实发育过程中总酚和类黄酮含量及抗氧化活性的动态变化[J].果树学报,2019,36(2):174-184.
	HUANG C H, LIAO G L, XIE M, et al.. Dynamic changes in total phenols, flavonoids and antioxidant capacity during fruit development of different kiwifruit cultivars [J]. J. Fruit Sci., 2019,36 (2): 174-184.

酚类物质Phenols	1，1-二苯基-2-苦肼基DPPH	2，2’-联氮-双-3-乙基苯并噻唑啉-6-磺酸ABTS⁺
总黄酮Total flavonoids	0.98^**	0.88^**
总酚Total phenol	0.99^**	0.90^**
熊果苷Arbutin	0.98^**	0.91^**
绿原酸Chlorogenic acid	0.98^**	0.92^**
儿茶素Catechin	0.98^**	0.84^**
表儿茶素Epicatechin	0.96^**	0.87^**
芦丁Rutin	0.72^*	0.73^*
槲皮葡萄糖苷Quercetin glucoside	0.81^**	0.82^**
槲皮素鼠李糖苷Quercetin rhamnoside	0.92^**	0.92^**
异鼠李素-3-O-芸香苷Isorhamnetin 3-O-rutinoside	0.88^**	0.87^**
异鼠李素-3-O-葡萄糖苷Isorhamnetin 3-O-glucoside	0.94^**	0.97^**
山奈酚-双脱氧己糖苷Kaempferol hexoside-dideoxyhexoside	0.90^**	0.92^**
山奈酚-3-O-葡萄糖苷Kaempferol 3-O-glucoside	0.95^**	0.93^**
山奈酚-3-O-芸香苷Kaempferol 3-O-rutinoside	0.85^**	0.75^*
异鼠李素酰化己糖苷Isorhamnetin-acylated-hexoside	0.95^**	0.88^**

酚类物质Phenols	1，1-二苯基-2-苦肼基DPPH	2，2’-联氮-双-3-乙基苯并噻唑啉-6-磺酸ABTS⁺
总黄酮Total flavonoids	0.98^**	0.88^**
总酚Total phenol	0.99^**	0.90^**
熊果苷Arbutin	0.98^**	0.91^**
绿原酸Chlorogenic acid	0.98^**	0.92^**
儿茶素Catechin	0.98^**	0.84^**
表儿茶素Epicatechin	0.96^**	0.87^**
芦丁Rutin	0.72^*	0.73^*
槲皮葡萄糖苷Quercetin glucoside	0.81^**	0.82^**
槲皮素鼠李糖苷Quercetin rhamnoside	0.92^**	0.92^**
异鼠李素-3-O-芸香苷Isorhamnetin 3-O-rutinoside	0.88^**	0.87^**
异鼠李素-3-O-葡萄糖苷Isorhamnetin 3-O-glucoside	0.94^**	0.97^**
山奈酚-双脱氧己糖苷Kaempferol hexoside-dideoxyhexoside	0.90^**	0.92^**
山奈酚-3-O-葡萄糖苷Kaempferol 3-O-glucoside	0.95^**	0.93^**
山奈酚-3-O-芸香苷Kaempferol 3-O-rutinoside	0.85^**	0.75^*
异鼠李素酰化己糖苷Isorhamnetin-acylated-hexoside	0.95^**	0.88^**

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