Abstract: In order to explore sustainable development of Dendrobium huoshanense resources, this paper used response surface method to optimize the protocorm multiplication medium. On the basis of singlefactor tests, the mathematical regression model was established about the dependent variable (proliferation rate) through BoxBenhnken center composite design and response surface analysis. The results showed that the concentration of NAA and 6benzylaminopurine (6BA) had the most significant effect on the proliferation rate, and the interaction between 6BA and potato was significant. The suitable concentrations of NAA, 6BA and potato were 0.05 mg·L-1, 0.70 mg·L-1 and 185 g·L-1, respectively. At this time, the proliferation rate was 2 989.82% +205.55%, which was consistent with the predicted value of the model. Above results indicated that the response surface methodology was viable for optimizing medium composition in improving proliferation rate.

Key words: Dendrobium huoshanense, Response surface methodology, Protocorm proliferation, Culture medium, Optimum formulation

摘要： 为可持续开发霍山石斛资源，采用响应面法优化霍山石斛原球茎增殖培养基。在单因素试验基础上，根据BoxBehnken试验设计原理，采用三因素三水平的响应面分析法，以增殖率为响应值进行回归分析。结果表明，萘乙酸（简称NAA）及6苄氨基嘌呤（简称6BA）浓度对增殖率影响最为显著，6BA与马铃薯两者间交互作用显著，适宜的NAA、6BA及马铃薯浓度分别为0.05 mg·L-1、0.70 mg·L-1和185 g·L-1，此时增殖率为2 989.82%±205.55%，与模型预测值基本符合。响应面法优化霍山石斛原球茎增殖培养基切实可行。

关键词: 霍山石斛, 响应面试验, 原球茎增殖, 培养基, 优化配方