Abstract:

Orthogonal experiments were applied to optimize the polysaccharide extraction from fruiting bodies and mycelium of Tricholoma mongolicum Imai. respectively. The optimal extraction parameters for fruiting bodies were predicted as followed: extraction temperature was 70℃, the ratio of raw material and water was 1∶10, extraction time was 4 hours, extraction was done  for 4 times and by 80% (V/V) of ethanol for polysaccharide precipitation. With this technics, the yield of polysaccharides was 5.78%. The optimum extraction conditions for mycelium  were extracted at temperature of 90℃, with ratio between raw material and water  as 1∶10. The extract would last for 3 hours, and be made for 3 times and by 80% ethanol for polysaccharide precipitation. With this technics, the yield of polysaccharides was 5.93%.

Key words: Tricholoma mongolicum Imai., polysaccharide, extraction technics

摘要：

以蒙古口蘑（Tricholoma mongolicum Imai.）野生子实体和人工液体培养的菌丝体为研究对象，采用常规热水浸提法，对影响多糖提取得率的因素以正交试验的方法进行优化。结果表明,蒙古口蘑子实体多糖提取的最佳工艺为:提取时间4 h，提取4次，提取温度70℃，醇析浓度80%，料液比1∶10,经此工艺子实体的多糖得率为5.78%;菌丝体多糖提取最优工艺为：提取时间3 h, 提取3次，提取温度90℃，醇析浓度80%，料液比1∶10,经此工艺菌丝体的多糖得率为5.93%。

关键词: 蒙古口蘑；多糖；提取工艺