Abstract: The conditions of ultrasonic-assisted extraction (UAE) of polysaccharides from Panax notoginseng root were optimized by response surface methodology. On the basis of single factor experiments, the extraction time, ultrasonic temperature, ratio of material to liquid and ultrasonic power were selected as the influencing factors. A 4-factor, 3-level Box-Behnken central composite design (CCD) was used for response surface analysis with polysaccharide yield as response value. The results showed that the optimal conditions of UAE were as follows: ultrasonic power of 320 W, extraction time of 41 min, temperature of 58℃, and material-liquid ratio 1 g∶50 mL. Under these conditions, the polysaccharides yield of Panax notoginseng root could reach 19.51%. Compared with the traditional hot water soaking and extracting method, the extraction efficiency of UAE was obviously improved. This technology was convenient, speedness with higher productivity. It could be used to guide the industrial production of extracting polysaccharide from Panax notoginseng root.

Key words: response surface method, ultrasound-assisted extraction, Panax notoginseng root, polysaccharide

摘要： 采用响应面法优化超声波提取三七根多糖的工艺条件。在单因素试验的基础上，选取提取时间、超声温度、料液比、超声功率为影响因子，以三七根多糖的产率作为响应值，应用Box-Behnken中心组合法进行4因素3水平的试验设计、响应面分析。结果表明，超声波提取三七根多糖的最优条件为：功率320 W, 时间41 min，温度58℃；料液比1 g∶50 mL，在此工艺条件下，多糖产率达到19.51%。与传统的热水浸提法相比提取效率明显提高，该工艺便捷、快速、产率较高，可用于指导工业生产提取三七根多糖。

关键词: 响应面法, 超声提取, 三七根, 多糖