Studies on Vitamin E in Foxtail Millet Determined by HPLC Method

›› 2009, Vol. 11 ›› Issue (1): 129-133.

Studies on Vitamin E in Foxtail Millet Determined by HPLC Method

LI Guo-ying1,2, FAN Zhi-ying3, LIU Fang1,2, ZHANG Ping3, LI Wei-xi1,2,
LIU Qing-sheng3, ZHU Zhi-hua,1,2

(1. National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences,
Chinese Academy of Agricultural Sciences, Beijing 100081|2. Supervision and Testing Center for Crop Germplasm
Resources, Ministry of Agriculture, Beijing 100081|3. Feed Research Institute, CAAS, Beijing 100081, China)

Received:2008-09-23 Revised:2008-11-06 Online:2009-02-15 Published:2009-07-29

高效液相色谱法测定谷子种质资源中维生素E的研究

李国营1,2,范志影3,刘方1,2,张萍3,李为喜1,2,刘庆生3,朱志华1,2

（1.中国农业科学院作物科学研究所, 国家农作物基因资源与遗传改良重大科学工程, 北京 100081； 2.农业部作物品种资源监督检验测试中心, 北京 100081； 3.中国农业科学院饲料研究所, 北京 100081）

通讯作者: 朱志华,研究员,从事作物品种资源监督检验研究。Tel: 010-62174058; E-mail: zhihuazhu@mail.caas.net.cn
作者简介:李国营,硕士研究生,研究方向为作物种质资源营养成分及其遗传多样性。E-mail:yingli-1982@163.com。
基金资助:
植物种质资源共享平台建设项目（2005DKA21001-07）；作物种质资源保护项目［NB08-2130135-(25-30)-21-07］资助。

Abstract

Abstract:

Foxtail millet is a food crop rich in vitamin E content. A technology detecting vitamin E content in foxtail millet by applying high performance liquid chromatography（HPLC）was established. The precision, accuracy and reproducibility of this technology were evaluated comprehensively by statistical theory. Afterwards, vitamin E contents of 400 accessions of foxtail millet germplasm were evaluated, and a group of foxtail millets with high vitamin E content was screened.

Key words: foxtail millet, germplasm resources, vitamin E, HPLC

摘要：

谷子是富含维生素E的粮食作物,建立了应用高效液相色谱法（HPLC）测定谷子种质资源中维生素E的检测技术,并应用统计学原理对方法的精密度、准确度、再现性等进行了较全面的考察和评价。利用该方法对400份谷子种质资源进行了鉴定、评价,并筛选出一批高维生素E含量的种质资源。

关键词: 谷子；种质资源；维生素E；高效液相色谱

CLC Number:

S816.51

LI Guo-ying1,2, FAN Zhi-ying3, LIU Fang1,2, ZHANG Ping3, LI Wei-xi1,2, . Studies on Vitamin E in Foxtail Millet Determined by HPLC Method[J]. , 2009, 11(1): 129-133.

李国营1,2,范志影3,刘方1,2,张萍3,李为喜1,2,刘庆生3,朱志华1,2. 高效液相色谱法测定谷子种质资源中维生素E的研究[J]. , 2009, 11(1): 129-133.

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