Genetic Diversity Analysis of 255 Maize Germplasm Resources Based on 10K SNP Chip

doi:10.13304/j.nykjdb.2023.0801

Abstract

Abstract:

Maize （Zea mays L.） is one of the world’s top three food crops and an important feed crop. Analyzing the agronomic phenotypic traits and genotypic genetic diversity of maize germplasm resources has important guiding significance for crop breeding and production. A natural population composed of 255 germplasm resources was used as the research material， and the genotyping was obtained by whole genome scanning using 10K SNP liquid chip. Phenotypic clustering and genetic diversity analysis were performed using VCF2PCA cluster and Mega11 software. The results showed that 14 139 high quality SNP sites were detected， which were distributed on 10 chromosomes of maize. The polymorphisms ranged from 0.05 to 0.50， with an average of 0.29. Polymorphism information content （PIC） varied from 0.04 to 0.38 with an average of 0.24. 104 SNPs with allele frequency difference were more than 0.30 between tropical and temperate populations. Through population genetic structure analysis， principal component analysis and cluster analysis， 255 samples were mainly divided into temperate and tropical subgroups. These results identified the genetic diversity and relationships among 255 germplasm resources from temperate and tropical maize， laying the foundation for subsequent maize germplasm improvement and innovation.

Key words: Zea mays L., 10K SNP, germplasm resources, genetic diversity analysis

摘要：

玉米（Zea mays L.）是全球三大粮食作物之一，也是重要的饲料作物。分析玉米种质资源的农艺性状和遗传多样性对玉米育种和生产具有重要指导意义。以255份玉米种质构成的自然群体作为研究材料，利用10K SNP液相芯片进行全基因组扫描，获取基因分型；利用VCF2PCA Cluster和Mega11等软件进行表型聚类和遗传多样性分析。结果表明，检测到14 139个高质量SNP位点，分布于玉米10条染色体上；基因多态性的变化范围为0.05~0.50，均值为0.29；多态信息含量（polymorphism information content， PIC）的变化范围为0.04~0.38，均值为0.24；热带群体和温带群体的等位基因频率差异绝对值大于0.30的位点有104个；通过群体遗传结构分析、主成分分析和聚类分析，将255份供试材料划分为温带和热带2个主要亚群。研究结果确定了255份来自温带和热带玉米种质间的遗传多样性和亲缘关系，为后续玉米种质资源改良和创新奠定基础。

关键词: 玉米, 10K SNP, 种质资源, 遗传多样性

CLC Number:

S513

Shan GAO, Xiaocui YAN, Nan WANG, Mengjie ZHANG, Youpeng LI, Wenda DIAO, Huijun DUAN. Genetic Diversity Analysis of 255 Maize Germplasm Resources Based on 10K SNP Chip[J]. Journal of Agricultural Science and Technology, 2024, 26(8): 20-33.

高山, 闫晓翠, 王楠, 张梦洁, 李友鹏, 刁文达, 段会军. 基于10K SNP芯片分析255份玉米种质资源的遗传多样性[J]. 中国农业科技导报, 2024, 26(8): 20-33.

Figures/Tables 11

References 23

1	刘希慧,刘文欣,张义荣,等.利用SSR分子标记鉴定若干玉米自交系的亲缘关系[J].分子植物育种,2005,3(2):179-187.
	LIU X H, LIU W X, ZHANG Y R, et al.. Genetic relationship among several maize inbreds revealed by SSR markers [J]. Mol. Plant Breeding, 2005,3(2):179-187.
2	程莉莉,刘丽君,林浩,等.玉米种质资源遗传多样性分析[J].东北农业大学学报,2010,41(9): 6-14.
	CHENG L L, LIU L J, LIN H, et al.. Analysis on genetic diversity of maize germplasm [J]. J. Norhteast Agric. Univ., 2010,41(9): 6-14.
3	邹成林,谭华,黄开健,等.基于SNP芯片划分CIMMYT和广西玉米自交系杂种优势群[J].分子植物育种,2022: 1-17.
	ZOU C L, TAN H, HUANG K J, et al.. Heterotic groups of CIMMYT and Guangxi maize inbred lines by SNP chips [J]. Mol. Plant Breeding, 2024,22(2): 480-489.
4	段灿星,董怀玉,李晓,等.玉米种质资源大规模多年多点多病害的自然发病抗性鉴定[J].作物学报,2020,46(8): 1135-1145.
	DUAN C X, DONG H Y, LI X, et al.. A large-scale screening of maize germplasm for resistance to multiple diseases in multi-plot demonstration for several years under natural condition [J]. Acta Agron. Sin., 2020,46(8): 1135-1145.
5	陈文娟,李万昌,杨知还,等.玉米抗南方锈病种质资源初步鉴定及遗传多样性分析[J].植物遗传资源学报,2018,19(2): 225-231, 242.
	CHEN W J, LI W C, YANG Z H, et al.. Preliminary identification and genetic diversity analysis of maize germplasm resources for resistance to southern corn rust [J]. J. Plant Genet. Resour., 2018,19(2): 225-231, 242.
6	王仕伟,李晓鹏,陈甲法,等.玉米抗蚜虫种质资源的鉴定[J].玉米科学,2018,26(2): 156-160, 165.
	WANG S W, LI X P, CHEN J F, et al.. Germplasm evaluation for Rhopalosiphummaidis (Fitch) resistance in maize [J]. J. Maize Sci., 2018,26(2):156-160, 165.
7	蒙祖庆,宋丰萍.西藏玉米地方品种表型性状的综合评价与特异种质筛选[J].西北农林科技大学学报,2017,45(8): 56-68, 75.
	MENG Z Q, SONG F P. Comprehensive evaluation of phenotypic characteristics and selection of specific germplasms of Tibet maize landraces [J]. J. Northwest A&F Univ. (Nat. Sci.), 2017,45(8): 56-68, 75.
8	徐云碧,王冰冰,张健,等.应用分子标记技术改进作物品种保护和监督[J].作物学报,2022,48(8): 1853-1870.
	XU Y B, WANG B B, ZHANG J, et al.. Enhancement of plant variety protection and regulation using molecular marker technology [J]. Acta Agron. Sin., 2022,48(8): 1853-1870.
9	马世鹏,张云龙,段民孝,等.基于Maize6H-60K 芯片精准分型的玉米DH群体遗传规律研究[J].中国农业大学学报,2022,27(11): 1-12.
	MA S P, ZHANG Y L, DUAN M X, et al.. Study on genetic rule of maize doubled haploid population based on Maize6H-60K accurate-genotyping [J]. J. China Agric. Univ., 2022,27(11): 1-12.
10	JOHES E S, SULLIVAN H, BHATTRAMAKKI D, et al.. A comparison of simple sequence repeat and single nucleotide polymorphism marker technologies for the genotypic analysis of maize (Zea mays L.) [J]. Theor. Appl. Genet., 2007,115(3): 361-371.
11	VARSHNEY R K, THIEL T, SRETENOVIC-RAIJCIC T, et al.. Identification and validation of a core set of informative genic SSR and SNP markers for assaying functional diversity in barley [J]. Mol. Breeding, 2008,22(1): 1-13.
12	ZHANG X, ZHANG H, LI L, et al.. Characterizing the population structure and genetic diversity of maize breeding germplasm in Southwest China using genome-wide SNP marker [J/OL]. BMC Genomics, 2016,17: 697 [2024-01-25]. .
13	杜雪,李国勤,卢立志.SNP分子标记研究进展[C]//全国生物遗传多样性高峰论坛论文集.昆明,2012:2.
14	唐立群,肖层林,王伟平.SNP分子标记的研究及其应用进展[J].中国农学通报,2012,28(12): 154-158.
	TANG L Q, XIAO C L, WANG W P. Research and application progress of SNP markers [J]. Chin. Agric. Sci. Bull., 2012,28(12): 154-158.
15	刘传光,张桂权.水稻单核苷酸多态性及其应用[J].遗传,2006 (6):737-744.
	LIU C G, ZHANG G Q. Single nucleotide polymorphism (SNP) and its application in rice [J]. Hereditas, 2006 (6):737-744.
16	杨宏霞.玉米自交系重要农艺性状的全基因组关联分析[D]. 榆林:榆林学院,2023.
	YANG H X. Genome-wide association analysis of important agronomic traits in maize inbred lines [D]. Yulin: Yulin University, 2023.
17	冷益丰.四川当前主要玉米种质杂种优势类群及产量配合力研究[D].雅安:四川农业大学, 2018.
	LENG Y F. Studies on heterotic grouping and combining ability of yield properties of current main maize germplasm in Sichuan province [D]. Ya’an: Sichuan Agricultural University,2018.
18	陈坚剑,张慧,王婷甄,等.甜玉米自交系遗传多样性和群体结构分析[J].分子植物育种,2022,20(19): 6559-6565.
	CHEN J J, ZHANG H, WANG T Z, et al.. Genetic diversity and population genetic structure analysis of sweet corn inbred lines[J]. Mol. Plant Breeding, 2022,20(19): 6559-6565.
19	KUMAR S, STECHER G, LI M, et al.. MEGA X: molecular evolutionary genetics analysis across computing platforms [J]. Mol. Biol. Evol., 2018,35(6): 1547-1549.
20	FALUSH D, STEPHENS M, PRITCHSRD J K. Inference of population structure using multiocus genotype data: linked loci and correlated allele frequencies [J]. Genetics, 2003,164(4): 1567-1587.
21	季维智,宿兵.遗传多样性研究的原理和方法[M].杭州:浙江科学技术出版社,1999:1-3.
22	吴金凤,宋伟,王蕊,等.利用SNP标记对51份玉米自交系进行类群划分[J].玉米科学,2014,22(5): 29-34.
	WU J F, SONG W, WANG R, et al.. Heterotic grouping of 51 maize inbred lines by SNP markers [J]. J. Maize Sci., 2014,22(5): 29-34.
23	谢和霞,范兢升,谢小东,等.基于SNP标记分析广西玉米地方品种遗传多样性[J].植物遗传资源学报,2023,24(6):1580-1590.
	XIE H X, FAN J S, XIE X D, et al.. SNP-based genetic diversity dissection of maize landraces from Guangxi [J]. J. Plant Genet. Resour., 2023,24(6):1580-1590.

染色体 Chromosome	数量 Number	密度 Density/Mb^-1	最小间隔 Minimum spacing/bp	最大间隔Maximum spacing/Mb
1	2 483	0.12	1	299.11
2	1 485	0.16	1	237.66
3	1 476	0.16	1	230.88
4	1 813	0.13	1	241.77
5	1 366	0.16	1	217.56
6	1 148	0.15	1	169.04
7	1 160	0.15	1	175.11
8	1 165	0.15	1	174.35
9	963	0.16	1	156.38
10	1 080	0.14	1	148.90
总计Total	14 139	0.15	1	205.08

染色体 Chromosome	数量 Number	密度 Density/Mb^-1	最小间隔 Minimum spacing/bp	最大间隔Maximum spacing/Mb
1	2 483	0.12	1	299.11
2	1 485	0.16	1	237.66
3	1 476	0.16	1	230.88
4	1 813	0.13	1	241.77
5	1 366	0.16	1	217.56
6	1 148	0.15	1	169.04
7	1 160	0.15	1	175.11
8	1 165	0.15	1	174.35
9	963	0.16	1	156.38
10	1 080	0.14	1	148.90
总计Total	14 139	0.15	1	205.08

序号 Serial number	编号 Code	名称 Name	杂合率 Heterozygosity rate	序号 Serial number	编号 Code	名称 Name	杂合率 Heterozyosity rate
1	D1	C86052	0.001 46	9	D108	D1139	0.074 46
2	D100	18599	0.001 73	10	D11	B8	0.003 48
3	D101	PHR62	0.002 66	11	D111	DH06	0.002 13
4	D102	PHT22	0.001 87	12	D112	DH17	0.001 60
5	D103	DF32	0.002 80	13	D114	DH33	0.019 67
6	D104	926	0.002 94	14	D116	DH104	0.004 78
7	D105	A801	0.002 39	15	D12	7236	0.002 26
8	D106	y9961	0.002 13	16	D120	1423 DS	0.000 93
17	D121	1432	0.001 33	53	D29	鉴1495b Jian 1495b	0.002 93
18	D122	5651	0.003 32	54	D31	91黄15 91 huang 15	0.002 80
19	D123	石1 Shi 1	0.043 78	55	D32	鹿65 Lu 65	0.002 27
20	D125	NC10	0.025 06	56	D33	岩38 Yan 38	0.055 99
21	D126	HN Sh-1	0.020 05	57	D35	78002A	0.000 80
22	D127	51 小21 51 xiao 21	0.001 86	58	D36	PB80	0.000 26
23	D128	丹昌Danchang	0.002 40	59	D37	N42	0.006 76
24	D13	旱21 Han 21	0.002 26	60	D38	802	0.002 13
25	D130	TJH	0.002 07	61	D40	旅28 Lyu 28	0.002 93
26	D131	15Z563	0.006 01	62	D41	CR1HT	0.085 07
27	D133	15H1280	0.003 19	63	D43	4683	0.001 87
28	D135	B075602	0.023 96	64	D44	864	0.002 66
29	D136	B075601	0.107 84	65	D46	农系5678 Nongxi 5678	0.072 76
30	D137	14N1005	0.002 92	66	D47	R548	0.051 22
31	D14	R1656	0.028 59	67	D48	DF20	0.000 40
32	D141	豫66♂ Yu 66♂	0.002 67	68	D50	d140	0.001 73
33	D142	农3435 Nong 3455	0.001 60	69	D51	101314	0.031 91
34	D143	68	0.029 94	70	D52	6M502	0.080 95
35	D144	653♂	0.002 13	71	D54	B73改B73 gai	0.000 40
36	D146	黄WX♀ Huang WX♀	0.026 41	72	D55	农系376 Nongxi 376	0.001 46
37	D149	9953	0.015 56	73	D56	e群4 e qun 4	0.002 80
38	D152	R99	0.001 73	74	D57	DF27	0.002 80
39	D153	BS110	0.002 00	75	D58	PHG86	0.001 46
40	D155	DK66	0.005 44	76	D6	Ay420	0.003 45
41	D156	农系531 Nongxi 531	0.001 60	77	D60	掖515 Ye 515	0.043 22
42	D16	DF24	0.001 60	78	D61	吉444 Ji 444	0.003 72
43	D17	85白64 85 bai 64	0.003 46	79	D62	207	0.001 46
44	D19	W64a	0.002 26	80	D64	1313	0.001 33
45	D2	806A	0.002 27	81	D65	3335	0.047 56
46	D21	LH162	0.003 33	82	D66	掖52106	0.040 91
47	D23	B394	0.002 93	83	D67	PHJ33	0.002 12
48	D24	NS501	0.002 13	84	D69	L069	0.002 80
49	D25	甘41	0.002 40	85	D72	选6 Xuan 6	0.005 46
50	D26	S53	0.001 60	86	D73	WIL901	0.011 18
51	D27	Va26	0.002 67	87	D74	PHH93	0.001 86
52	D28	辐8527 Fu 8527	0.002 13	88	D75	7903E	0.002 53
89	D76	三北8 Sanbei 8	0.001 73	125	GS397	DTMA12	0.002 00
90	D78	辐射8701Fushe 8701	0.002 39	126	GS398	DTMA13	0.001 60
91	D79	BCC03	0.002 53	127	GS400	DTMA17	0.001 73
92	D8	太184 Tai 184	0.003 46	128	S10	DTMA38	0.002 68
93	D80	祈轮59 Qilun 59	0.002 58	129	S111	DTMA24	0.004 93
94	D81	1614	0.016 48	130	S13	DTMA43	0.006 02
95	D82	掖488 Ye 488	0.083 08	131	S14	DTMA46	0.003 21
96	D83	2369	0.002 66	132	S142	DTMA16	0.002 13
97	D86	B100	0.003 19	133	S15	DTMA49	0.003 47
98	D87	E600	0.002 00	134	S163	DTMA126	0.002 81
99	D89	黄野四 Huangyesi	0.002 81	135	S165	DTMA134	0.003 35
100	D9	Lx9801	0.003 32	136	S17	DTMA61	0.002 67
101	D90	LH196	0.000 93	137	S19	DTMA69	0.001 87
102	D91	D33A	0.003 72	138	S192	DTMA20	0.002 14
103	D92	WIL500	0.001 73	139	S193	DTMA50	0.001 88
104	D93	R31	0.001 73	140	S2	DTMA5	0.001 88
105	D97	P25	0.001 06	141	S203	DTMA45	0.035 13
106	GS049	DTMA58	0.032 23	142	S206	DTMA39	0.002 94
107	GS096	DTMA22	0.004 81	143	S208	DTMA83	0.003 47
108	GS110	DTMA19	0.002 26	144	S209	DTMA151	0.001 73
109	GS111	DTMA222	0.002 94	145	S21	DTMA80	0.002 28
110	GS138	DTMA254	0.002 93	146	S214	DTMA15	0.002 14
111	GS158	郑58 Zheng 58	0.001 33	147	S22	DTMA82	0.002 67
112	GS160	X178	0.002 26	148	S25	DTMA95	0.003 93
113	GS162	QI319	0.002 39	149	S27	DTMA101	0.002 43
114	GS163	掖478 Ye 478	0.000 93	150	S28	DTMA102	0.002 54
115	GS164	丹340 Dan 340	0.001 86	151	S29	DTMA108	0.002 27
116	GS165	黄早四Huangzaosi	0.002 66	152	S3	DTMA8	0.066 52
117	GS166	Tie7922	0.000 93	153	S35	DTMA127	0.001 74
118	GS168	Mo17	0.002 80	154	S36	DTMA132	0.002 41
119	GS169	吉81162 Ji 81162	0.002 27	155	S40	DTMA145	0.005 47
120	GS170	豫12 Yu 12	0.004 52	156	S44	DTMA164	0.002 54
121	GS172	DTMA25	0.002 27	157	S45	DTMA166	0.003 75
122	GS173	QI318	0.001 73	158	S46	DTMA185	0.002 27
123	GS328	DTMA54	0.002 26	159	S47	DTMA189	0.002 67
124	GS391	DTMA3	0.002 40	160	S49	DTMA191	0.002 27
161	S50	DTMA192	0.002 13	197	W126	DTMA142	0.002 68
162	S51	DTMA193	0.004 41	198	W128	DTMA144	0.003 60
163	S52	DTMA194	0.002 67	199	W130	DTMA147	0.002 28
164	S53	DTMA195	0.045 12	200	W132	DTMA150	0.002 27
165	S54	DTMA200	0.002 81	201	W133	DTMA152	0.003 07
166	S57	DTMA207	0.003 07	202	W137	DTMA156	0.002 40
167	S59	DTMA210	0.003 09	203	W143	DTMA162	0.003 46
168	S6	DTMA26	0.002 28	204	W144	DTMA163	0.002 93
169	S60	DTMA211	0.002 01	205	W146	DTMA165	0.002 27
170	S61	DTMA213	0.003 21	206	W148	DTMA167	0.003 07
171	S7	DTMA35	0.002 95	207	W149	DTMA168	0.002 67
172	S8	DTMA36	0.002 80	208	W151	DTMA170	0.002 27
173	S81	DTMA215	0.002 67	209	W152	DTMA171	0.002 53
174	S82	DTMA41	0.002 00	210	W154	DTMA173	0.001 34
175	S86	DTMA257	0.002 81	211	W156	DTMA175	0.002 40
176	S87	DTMA226	0.002 81	212	W161	DTMA188	0.003 61
177	S88	DTMA93	0.002 94	213	W23	DTMA28	0.008 14
178	S92	DTMA96	0.003 20	214	W24	DTMA29	0.002 00
179	S94	DTMA32	0.009 94	215	W25	DTMA30	0.002 27
180	W100	DTMA113	0.006 98	216	W26	DTMA31	0.005 88
181	W101	DTMA114	0.002 39	217	W38	DTMA47	0.004 00
182	W102	DTMA115	0.002 93	218	W39	DTMA48	0.001 74
183	W103	DTMA117	0.003 47	219	W42	DTMA51	0.002 93
184	W106	DTMA120	0.005 20	220	W46	DTMA55	0.002 27
185	W107	DTMA121	0.051 70	221	W47	DTMA56	0.002 53
186	W109	DTMA123	0.003 74	222	W48	DTMA57	0.001 88
187	W111	DTMA125	0.003 47	223	W50	DTMA59	0.008 26
188	W114	DTMA128	0.002 40	224	W51	DTMA60	0.093 41
189	W115	DTMA129	0.001 86	225	W54	DTMA63	0.002 13
190	W116	DTMA130	0.002 00	226	W55	DTMA64	0.002 27
191	W117	DTMA131	0.002 27	227	W56	DTMA65	0.001 47
192	W119	DTMA133	0.002 93	228	W57	DTMA66	0.002 14
193	W121	DTMA136	0.002 41	229	W59	DTMA68	0.003 34
194	W122	DTMA138	0.002 81	230	W61	DTMA70	0.001 74
195	W124	DTMA140	0.002 00	231	W62	DTMA71	0.001 60
196	W125	DTMA141	0.003 07	232	W63	DTMA72	0.001 73
233	W64	DTMA73	0.034 73	245	W80	DTMA92	0.018 48
234	W65	DTMA74	0.002 01	246	W85	DTMA97	0.002 67
235	W66	DTMA75	0.001 73	247	W86	DTMA99	0.003 21
236	W67	DTMA76	0.007 21	248	W87	DTMA100	0.002 67
237	W68	DTMA77	0.005 87	249	W90	DTMA103	0.002 53
238	W69	DTMA78	0.003 21	250	W93	DTMA106	0.003 99
239	W70	DTMA79	0.003 47	251	W94	DTMA107	0.002 67
240	W72	DTMA81	0.007 73	252	W96	DTMA109	0.003 20
241	W74	DTMA84	0.002 67	253	W97	DTMA110	0.002 27
242	W76	DTMA86	0.003 34	254	W98	DTMA111	0.002 00
243	W77	DTMA87	0.001 87	255	W99	DTMA112	0.001 47
244	W78	DTMA88	0.087 17

序号 Serial number	编号 Code	名称 Name	杂合率 Heterozygosity rate	序号 Serial number	编号 Code	名称 Name	杂合率 Heterozyosity rate
1	D1	C86052	0.001 46	9	D108	D1139	0.074 46
2	D100	18599	0.001 73	10	D11	B8	0.003 48
3	D101	PHR62	0.002 66	11	D111	DH06	0.002 13
4	D102	PHT22	0.001 87	12	D112	DH17	0.001 60
5	D103	DF32	0.002 80	13	D114	DH33	0.019 67
6	D104	926	0.002 94	14	D116	DH104	0.004 78
7	D105	A801	0.002 39	15	D12	7236	0.002 26
8	D106	y9961	0.002 13	16	D120	1423 DS	0.000 93
17	D121	1432	0.001 33	53	D29	鉴1495b Jian 1495b	0.002 93
18	D122	5651	0.003 32	54	D31	91黄15 91 huang 15	0.002 80
19	D123	石1 Shi 1	0.043 78	55	D32	鹿65 Lu 65	0.002 27
20	D125	NC10	0.025 06	56	D33	岩38 Yan 38	0.055 99
21	D126	HN Sh-1	0.020 05	57	D35	78002A	0.000 80
22	D127	51 小21 51 xiao 21	0.001 86	58	D36	PB80	0.000 26
23	D128	丹昌Danchang	0.002 40	59	D37	N42	0.006 76
24	D13	旱21 Han 21	0.002 26	60	D38	802	0.002 13
25	D130	TJH	0.002 07	61	D40	旅28 Lyu 28	0.002 93
26	D131	15Z563	0.006 01	62	D41	CR1HT	0.085 07
27	D133	15H1280	0.003 19	63	D43	4683	0.001 87
28	D135	B075602	0.023 96	64	D44	864	0.002 66
29	D136	B075601	0.107 84	65	D46	农系5678 Nongxi 5678	0.072 76
30	D137	14N1005	0.002 92	66	D47	R548	0.051 22
31	D14	R1656	0.028 59	67	D48	DF20	0.000 40
32	D141	豫66♂ Yu 66♂	0.002 67	68	D50	d140	0.001 73
33	D142	农3435 Nong 3455	0.001 60	69	D51	101314	0.031 91
34	D143	68	0.029 94	70	D52	6M502	0.080 95
35	D144	653♂	0.002 13	71	D54	B73改B73 gai	0.000 40
36	D146	黄WX♀ Huang WX♀	0.026 41	72	D55	农系376 Nongxi 376	0.001 46
37	D149	9953	0.015 56	73	D56	e群4 e qun 4	0.002 80
38	D152	R99	0.001 73	74	D57	DF27	0.002 80
39	D153	BS110	0.002 00	75	D58	PHG86	0.001 46
40	D155	DK66	0.005 44	76	D6	Ay420	0.003 45
41	D156	农系531 Nongxi 531	0.001 60	77	D60	掖515 Ye 515	0.043 22
42	D16	DF24	0.001 60	78	D61	吉444 Ji 444	0.003 72
43	D17	85白64 85 bai 64	0.003 46	79	D62	207	0.001 46
44	D19	W64a	0.002 26	80	D64	1313	0.001 33
45	D2	806A	0.002 27	81	D65	3335	0.047 56
46	D21	LH162	0.003 33	82	D66	掖52106	0.040 91
47	D23	B394	0.002 93	83	D67	PHJ33	0.002 12
48	D24	NS501	0.002 13	84	D69	L069	0.002 80
49	D25	甘41	0.002 40	85	D72	选6 Xuan 6	0.005 46
50	D26	S53	0.001 60	86	D73	WIL901	0.011 18
51	D27	Va26	0.002 67	87	D74	PHH93	0.001 86
52	D28	辐8527 Fu 8527	0.002 13	88	D75	7903E	0.002 53
89	D76	三北8 Sanbei 8	0.001 73	125	GS397	DTMA12	0.002 00
90	D78	辐射8701Fushe 8701	0.002 39	126	GS398	DTMA13	0.001 60
91	D79	BCC03	0.002 53	127	GS400	DTMA17	0.001 73
92	D8	太184 Tai 184	0.003 46	128	S10	DTMA38	0.002 68
93	D80	祈轮59 Qilun 59	0.002 58	129	S111	DTMA24	0.004 93
94	D81	1614	0.016 48	130	S13	DTMA43	0.006 02
95	D82	掖488 Ye 488	0.083 08	131	S14	DTMA46	0.003 21
96	D83	2369	0.002 66	132	S142	DTMA16	0.002 13
97	D86	B100	0.003 19	133	S15	DTMA49	0.003 47
98	D87	E600	0.002 00	134	S163	DTMA126	0.002 81
99	D89	黄野四 Huangyesi	0.002 81	135	S165	DTMA134	0.003 35
100	D9	Lx9801	0.003 32	136	S17	DTMA61	0.002 67
101	D90	LH196	0.000 93	137	S19	DTMA69	0.001 87
102	D91	D33A	0.003 72	138	S192	DTMA20	0.002 14
103	D92	WIL500	0.001 73	139	S193	DTMA50	0.001 88
104	D93	R31	0.001 73	140	S2	DTMA5	0.001 88
105	D97	P25	0.001 06	141	S203	DTMA45	0.035 13
106	GS049	DTMA58	0.032 23	142	S206	DTMA39	0.002 94
107	GS096	DTMA22	0.004 81	143	S208	DTMA83	0.003 47
108	GS110	DTMA19	0.002 26	144	S209	DTMA151	0.001 73
109	GS111	DTMA222	0.002 94	145	S21	DTMA80	0.002 28
110	GS138	DTMA254	0.002 93	146	S214	DTMA15	0.002 14
111	GS158	郑58 Zheng 58	0.001 33	147	S22	DTMA82	0.002 67
112	GS160	X178	0.002 26	148	S25	DTMA95	0.003 93
113	GS162	QI319	0.002 39	149	S27	DTMA101	0.002 43
114	GS163	掖478 Ye 478	0.000 93	150	S28	DTMA102	0.002 54
115	GS164	丹340 Dan 340	0.001 86	151	S29	DTMA108	0.002 27
116	GS165	黄早四Huangzaosi	0.002 66	152	S3	DTMA8	0.066 52
117	GS166	Tie7922	0.000 93	153	S35	DTMA127	0.001 74
118	GS168	Mo17	0.002 80	154	S36	DTMA132	0.002 41
119	GS169	吉81162 Ji 81162	0.002 27	155	S40	DTMA145	0.005 47
120	GS170	豫12 Yu 12	0.004 52	156	S44	DTMA164	0.002 54
121	GS172	DTMA25	0.002 27	157	S45	DTMA166	0.003 75
122	GS173	QI318	0.001 73	158	S46	DTMA185	0.002 27
123	GS328	DTMA54	0.002 26	159	S47	DTMA189	0.002 67
124	GS391	DTMA3	0.002 40	160	S49	DTMA191	0.002 27
161	S50	DTMA192	0.002 13	197	W126	DTMA142	0.002 68
162	S51	DTMA193	0.004 41	198	W128	DTMA144	0.003 60
163	S52	DTMA194	0.002 67	199	W130	DTMA147	0.002 28
164	S53	DTMA195	0.045 12	200	W132	DTMA150	0.002 27
165	S54	DTMA200	0.002 81	201	W133	DTMA152	0.003 07
166	S57	DTMA207	0.003 07	202	W137	DTMA156	0.002 40
167	S59	DTMA210	0.003 09	203	W143	DTMA162	0.003 46
168	S6	DTMA26	0.002 28	204	W144	DTMA163	0.002 93
169	S60	DTMA211	0.002 01	205	W146	DTMA165	0.002 27
170	S61	DTMA213	0.003 21	206	W148	DTMA167	0.003 07
171	S7	DTMA35	0.002 95	207	W149	DTMA168	0.002 67
172	S8	DTMA36	0.002 80	208	W151	DTMA170	0.002 27
173	S81	DTMA215	0.002 67	209	W152	DTMA171	0.002 53
174	S82	DTMA41	0.002 00	210	W154	DTMA173	0.001 34
175	S86	DTMA257	0.002 81	211	W156	DTMA175	0.002 40
176	S87	DTMA226	0.002 81	212	W161	DTMA188	0.003 61
177	S88	DTMA93	0.002 94	213	W23	DTMA28	0.008 14
178	S92	DTMA96	0.003 20	214	W24	DTMA29	0.002 00
179	S94	DTMA32	0.009 94	215	W25	DTMA30	0.002 27
180	W100	DTMA113	0.006 98	216	W26	DTMA31	0.005 88
181	W101	DTMA114	0.002 39	217	W38	DTMA47	0.004 00
182	W102	DTMA115	0.002 93	218	W39	DTMA48	0.001 74
183	W103	DTMA117	0.003 47	219	W42	DTMA51	0.002 93
184	W106	DTMA120	0.005 20	220	W46	DTMA55	0.002 27
185	W107	DTMA121	0.051 70	221	W47	DTMA56	0.002 53
186	W109	DTMA123	0.003 74	222	W48	DTMA57	0.001 88
187	W111	DTMA125	0.003 47	223	W50	DTMA59	0.008 26
188	W114	DTMA128	0.002 40	224	W51	DTMA60	0.093 41
189	W115	DTMA129	0.001 86	225	W54	DTMA63	0.002 13
190	W116	DTMA130	0.002 00	226	W55	DTMA64	0.002 27
191	W117	DTMA131	0.002 27	227	W56	DTMA65	0.001 47
192	W119	DTMA133	0.002 93	228	W57	DTMA66	0.002 14
193	W121	DTMA136	0.002 41	229	W59	DTMA68	0.003 34
194	W122	DTMA138	0.002 81	230	W61	DTMA70	0.001 74
195	W124	DTMA140	0.002 00	231	W62	DTMA71	0.001 60
196	W125	DTMA141	0.003 07	232	W63	DTMA72	0.001 73
233	W64	DTMA73	0.034 73	245	W80	DTMA92	0.018 48
234	W65	DTMA74	0.002 01	246	W85	DTMA97	0.002 67
235	W66	DTMA75	0.001 73	247	W86	DTMA99	0.003 21
236	W67	DTMA76	0.007 21	248	W87	DTMA100	0.002 67
237	W68	DTMA77	0.005 87	249	W90	DTMA103	0.002 53
238	W69	DTMA78	0.003 21	250	W93	DTMA106	0.003 99
239	W70	DTMA79	0.003 47	251	W94	DTMA107	0.002 67
240	W72	DTMA81	0.007 73	252	W96	DTMA109	0.003 20
241	W74	DTMA84	0.002 67	253	W97	DTMA110	0.002 27
242	W76	DTMA86	0.003 34	254	W98	DTMA111	0.002 00
243	W77	DTMA87	0.001 87	255	W99	DTMA112	0.001 47
244	W78	DTMA88	0.087 17

染色体 Chromosome	基因多态性范围 D range	基因多态性 D	多态信息含量范围 PIC range	多态信息含量 PIC
1	0.07~0.50	0.30	0.07~0.38	0.24
2	0.05~0.50	0.30	0.04~0.38	0.25
3	0.07~0.50	0.27	0.07~0.38	0.23
4	0.06~0.50	0.31	0.06~0.38	0.25
5	0.07~0.50	0.27	0.07~0.37	0.23
6	0.06~0.50	0.31	0.06~0.38	0.25
7	0.07~0.50	0.28	0.07~0.37	0.24
8	0.07~0.50	0.31	0.07~0.38	0.25
9	0.07~0.50	0.30	0.07~0.38	0.24
10	0.07~0.50	0.29	0.07~0.38	0.24
总计Total	0.05~0.50	0.29	0.04~0.38	0.24