Genetic Transformation of Maize Embryonic Callus and Regeneration of Glyphosate-tolerant Plant

doi:10.3969/j.issn.1008-0864.2010.04.20

›› 2010, Vol. 12 ›› Issue (4): 108-113.DOI: 10.3969/j.issn.1008-0864.2010.04.20

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Genetic Transformation of Maize Embryonic Callus and Regeneration of Glyphosate-tolerant Plant

YU Gui-rong1,2, DU Wen-ping2, SONG Jun2, DOU Qian2, LIU Yong-sheng1, LU Wei3, XU Li-yuan2

(1.College of Life Sciences, Sichuan University, Chengdu 610064|2.Institute of Biotechnology &|Nuclear Techniques,
Sichuan Academy of Agricultural Sciences, Chengdu 610066|3.Biotechnology Research Institute, Chinese Academy of
Agricultural Sciences, Beijing 100081, China)

Received:2010-04-23 Revised:2010-06-02 Online:2010-08-15 Published:2010-07-23

玉米胚性愈伤组织的遗传转化及耐草甘膦植株再生

余桂容1,2,杜文平2,宋军2|窦茜2,刘永胜1,陆伟3,徐利远2

(1.四川大学生命科学院, 成都 610064|2.四川省农业科学院生物技术核技术研究所, 成都 610066|3.中国农业科学院生物技术研究所, 北京 100081)

通讯作者: 徐利远,研究员,主要从事基因工程育种研究。E-mail:yugui100@hotmail.com
作者简介:余桂容,副研究员,博士研究生,主要从事基因工程育种。
基金资助:
转基因生物新品种培育重大专项(2009ZX08003-003B);四川省农科院优秀论文基金项目(2008-2010转耐草甘膦抗虫玉米研究)资助。

Abstract

Abstract:

2mG2-epsps gene from Pseudomonas fluorescens G2 was transferred to immature embryo-derived callus of the elite maize inbred line X2211 via Agrobacterium-mediated transformation. 282 transformed plants were regenerated from glyphosate-resistant callus, 86 plants of which were grown well in the field. Glyphosate resistance and allele-specific PCR analysis demonstrated that the 2mG2-epsps gene was transferred into the maize genome in 26 regeneration plants. The plants showed resistance after spraying 0.125% glyphosate, which indicated the expression of 2mG2-epsps gene in the plants. Some of the transgenic plants gained high tolerance to glyphosate. This study has provided good parental materials for breeding new glyphosate-resistant maize varieties.

Key words: maize immature embryo, genetic transformation, glyphosate, 2mG2-epsps gene

摘要：

以优良玉米自交系X2211的幼胚胚性愈伤组织为受体材料,采用农杆菌介导法将来自微生物荧光假单胞杆菌(Pseudomonas fluorescens)G2菌株的抗除草剂基因2mG2-epsps转入玉米愈伤组织细胞,转化后的细胞经草甘膦筛选,获得抗性愈伤组织并再生植株282株,成活86株,经特异PCR检测表明其中26株植株转入了2mG2-epsps基因,喷施0.125%除草剂试验检测表明,转基因植株具有良好的耐除草剂特性,为抗除草剂玉米新品种选育提供了较好的亲本材料。

关键词: 玉米幼胚;遗传转化;草甘膦;2mG2-epsps基因

CLC Number:

Q758

YU Gui-rong1,2, DU Wen-ping2, SONG Jun2, DOU Qian2, LIU Yong-sheng1, LU Wei3, XU. Genetic Transformation of Maize Embryonic Callus and Regeneration of Glyphosate-tolerant Plant[J]. , 2010, 12(4): 108-113.

余桂容1,2,杜文平2,宋军2|窦茜2,刘永胜1,陆伟3,徐利远2 . 玉米胚性愈伤组织的遗传转化及耐草甘膦植株再生[J]. , 2010, 12(4): 108-113.

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Genetic Transformation of Maize Embryonic Callus and Regeneration of Glyphosate-tolerant Plant

玉米胚性愈伤组织的遗传转化及耐草甘膦植株再生

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