Abstract:

The CBF gene cloned from cotton was transformed into tobacco so as to study its impacts on tobacco. The PCR results showed that the CBF gene was successfully transferred into tobacco, and the transcription and expression of the exogenous CBF gene in transgenic tobacco were indentified by RT-PCR. Then the physiological indexes of stress resistance in the transgenic tobaccos were determined. The results showed that the soluble sugar content and POD activity of transgenic tobacco were significantly higher than the wild-type tobacco, reaching to 1.22~7.91 times and 3.35~5.73 times, respectively. And the MDA content was obviously lower than that of the wild type, taking 36.1% ~ 81.1% of the wild-type. These results indicated that CBF gene transformation had indeed improved the stress resistance of tobacco.

Key words: CBF, stress tolerance, soluble sugar, MDA, POD

摘要：

将棉花CBF基因转化入烟草,研究转CBF基因对烟草耐逆性的影响。对经PCR鉴定的阳性转基因烟草植株进行RT-PCR,结果表明外源CBF基因在转基因烟草中能够正常转录并表达。测定转基因烟草的各抗逆性相关生理指标,结果表明,转基因烟草的丙二醛含量明显低于野生型,为野生型的36.1%~81.1%；可溶性糖含量和POD活性均明显高于野生型烟草,分别达1.22~7.91倍和3.35~5.73倍,表明转CBF基因确实提高了烟草的耐逆性。

关键词: CBF耐逆性;可溶性糖;丙二醛;过氧化物酶