Development of CRISPR/Cas9 System and Its Aapplication in Plants

doi:10.13304/j.nykjdb.2021.0475

Journal of Agricultural Science and Technology ›› 2021, Vol. 23 ›› Issue (12): 20-28.DOI: 10.13304/j.nykjdb.2021.0475

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Development of CRISPR/Cas9 System and Its Aapplication in Plants

LIAO Jiaming1,2§, LI Chunmei1§, ZHANG Shihu1, LI Buye1, OUYANG Kunxi1*, CHEN Xiaoyang1*

1.College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China;
2.Forestry Bureau of Wuhua County, Guangdong Meizhou 514400, China

Received:2021-06-10 Accepted:2021-08-23 Online:2021-12-15 Published:2021-12-21

CRISPR/Cas9基因编辑技术的发展及其在植物中的应用

廖嘉明1,2§,李春梅1§,张石虎1,李布野1,欧阳昆唏1*,陈晓阳1*

1.华南农业大学林学与风景园林学院, 广州 510642; 2.五华县林业局, 广东梅州 514400

通讯作者: 陈晓阳 E-mail: xychen@scau.edu.cn；欧阳昆唏 E-mail: kxouyang@scau.edu.cn
作者简介:廖嘉明和李春梅为本文共同第一作者。廖嘉明 E-mail:1054737896@qq.com；李春梅 E-mail:lichunmeidage@163.com
基金资助:
广东省科技计划项目(2017B020201008)；

国家自然科学基金项目(31600525)；

中央财政林业科技推广示范项目(〔2018〕GDTK-08)；

广东省林业科技创新项目(2019KJCX001)

Abstract

Abstract: CRISPR/Cas9 system can edit target genes in a targeted way, which has great potential value in plant gene function research and genetic improvement. Because of its high efficiency, convenient operation and low cost, CRISPR/Cas9 system has become the most mainstream genome editing technology at present.In this paper, the origin, classification and mechanism of CRISPR/Cas9 system and its application in gene function verification and genetic improvement in crops and trees were briefly summarized, and the related problems and development trends needed to be improved in the future were also discussed.

Key words: CRISPR/Cas9 system, gene editing, plant genomes, gene function, genetic improvement

摘要： CRISPR/Cas9系统可以对目标基因进行定向编辑，在植物基因功能研究和遗传改良中具有巨大的潜在应用价值，因其具有高效、操作便捷及成本低的特点，成为当前基因组编辑技术的主流。概述了CRISPR/Cas9系统的起源、分类、作用机制及其在农作物和林木基因功能验证和遗传改良中的应用，并对未来需要完善的相关问题和发展趋势进行了探讨。

关键词: CRISPR/Cas9系统, 基因编辑, 植物基因组, 基因功能, 遗传改良

LIAO Jiaming, §, LI Chunmei§, ZHANG Shihu, LI Buye, OUYANG Kunxi, CHEN Xiaoyang. Development of CRISPR/Cas9 System and Its Aapplication in Plants[J]. Journal of Agricultural Science and Technology, 2021, 23(12): 20-28.

廖嘉明, §, 李春梅§, 张石虎, 李布野, 欧阳昆唏, 陈晓阳. CRISPR/Cas9基因编辑技术的发展及其在植物中的应用[J]. 中国农业科技导报, 2021, 23(12): 20-28.

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Development of CRISPR/Cas9 System and Its Aapplication in Plants

CRISPR/Cas9基因编辑技术的发展及其在植物中的应用

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