Abstract: YABBY family is plant-specific transcription factors and plays important roles in the regulation of lateral organ polarity and growth. In silico identification, bioinformatics and expression analysis of YABBY family genes in wheat (Trticum aestivum L.) can provide theoretical basis for exploring the roles of YABBY transcription factors in wheat. The YABBY genes in wheat were genome-widely identified in the wheat genome database according to sequences of YABBY genes from Arabidopsis and rice by BLAST software. Bioinformatics analysis of YABBY were carried out by MEME, GSDS, MEGA and PlantCARE softwares. The expression profiles were drawn based on the data from public RNA-Seq results. Total of 18 YABBY genes were identified from wheat genome, which were divided into 5 classes. Motif analysis showed that the TaYABBY proteins had C2C2 and YABBY conserved motifs. Multiple cis-elements were found in 1 000 upstream sequences of TaYABBY genes which responsed to stress and hormone response. The expression profile showed that TaYABBY genes had tissue specificity.

Key words: YABBY gene family, wheat, bioinformatics, phylogenetic analysis, gene function

摘要： YABBY家族是植物特有的转录因子，在植物侧生器官极性建立和发育过程中起重要的调控作用。从全基因组水平鉴定小麦YABBY家族并进行生物信息学和表达模式分析，为研究小麦YABBY家族基因的功能奠定基础。根据已经报道的拟南芥和水稻YABBY基因，在小麦基因组数据库中执行本地BLAST程序鉴定小麦基因组中的YABBY基因,采用MEGA、GSDS、MEME和PlantCARE等软件进行生物信息学分析,并利用已公开的RNA-seq数据绘制不同发育时期和不同组织的表达谱。结果表明，在小麦基因组范围内鉴定得到18个YABBY基因家族成员，分成5个亚家族。Motif分析表明小麦YABBY蛋白均具有C2C2锌指结构域及YABBY结构域；TaYABBY启动子区检测到与植物生长发育、激素诱导和逆境胁迫有关的顺式作用元件。RNA-Seq表达谱发现小麦YABBY基因具有明显的组织表达特异性。

关键词: YABBY基因家族, 小麦, 生物信息, 进化分析, 基因功能