Abstract: Cytoplasm male sterility (CMS) has widely been applied in agricultural breeding. Maize CMS lines have already played and will be continuously playing an indispensable and important role in new maize variety breeding and maize industrialization. This study discovered a cytoplasmic male-sterile line named CF3 developed by crossing of 2 maize inbred lines C7-2 and PH4CV. Furthermore, the derived sterile line CZ58 was obtained. Phenotype investigation and cytolog observation showed that the anthers of CMS lines, CF3 and CZ58 were not exposed. CF3 and CZ58 could generate a little bits of pollens. However, their pollen presented irregular translucent, and had no activity. Results of both PCR and sequencing showed that CF3 and CZ58 were novel CMS-S types. Genetics analysis suggested that the tassel presented clear infertility and stability. CF3 and CZ58 could be restored by various inbred lines, indicating that CF3 was a new S type cytoplasmic male-sterile line. CF3 and its derived line CZ58 had better applying potential in maize cross breeding and extension.

Key words: maize, cytoplasm male sterility (CMS), pollen, mitochondria, hybrid vigor

摘要： 细胞质雄性不育（cytoplasm male sterility，CMS）在作物杂种优势中具有广泛的应用。玉米CMS系在商业玉米新品种培育及产业化中已经并必将继续发挥不可或缺的重要作用。在两个玉米自交系C7-2和PH4CV杂交后代中发现一个胞质雄性不育系CF3，并进一步获得其衍生不育系CZ58。表型调查和细胞学观察结果表明CF3及CZ58不育系的花药不外露，可以产生少量花粉，但花粉粒呈现不规则透明状，无活性；特异性PCR及测序结果表明CF3不育系的线粒体为S型，遗传学分析表明其雄花败育比较彻底，不育性状稳定，恢复系比较广泛，说明CF3是一种新的S型胞质雄性不育系，CF3及其衍生系CZ58在玉米杂交种培育与推广中具有较好的应用潜力。

关键词: 玉米, 细胞质雄性不育, 花粉, 线粒体, 杂种优势