Abstract: In order to analyze physiological response to Pb stress and Pb distribution characteristics in subcellula tissues of different tea plants, this paper did research taking Tieguanyin and Rougui as materials by pot planting method, so as to provide theoretical basis for studying toxicity mechanism of heavy metal to tea plants and for self-defense of tea plants. The results showed that activities of leaf blade superoxide dismutase(SOD), peroxidase(POD), catalase (CAT) declined with the increase of Pb concentration, and the tolerance of Rougui to Pb stress was higher than that of Tieguanyin. There was no significant differences between the total Pb contents in subcellula tissues of the 2 tea plants under the same concentration of Pb stress. In addition, Tieguanyin increased Pb2+ propertion in cytosol and organelles to reduce Pb2+ toxicity, while Rougui mainly improved binding ratio of Pb2+. Therefore, there was different detoxification patterns for different tea plants under Pb stress.

Key words: tea plant, Pb stress, physiological characteristics, subcellular fraction, distribution characteristics

摘要： 为了分析不同茶树对Pb胁迫的生理响应及其组织亚细胞Pb分布特性，采用盆栽种植法，以铁观音、肉桂两种茶树为材料进行了分析，以期为重金属对茶树毒害机理和茶树对重金属的自我防御研究提供理论依据。结果表明：随着Pb胁迫浓度的增加，茶树叶片超氧化物歧化酶(superoxide dismutase，SOD)、过氧化物酶 (peroxidase，POD)、过氧化氢酶 (catalase，CAT)活性呈现下降趋势，且肉桂对Pb胁迫的耐受性高于铁观音；相同浓度Pb胁迫下，两种茶树不同组织亚细胞中的总Pb含量显著差异；此外，Pb胁迫下，为降低Pb2+毒害，铁观音茶树表现为提高植物细胞溶质与细胞器中的Pb2+比例，肉桂茶树表现为增加植物细胞壁中的Pb2+结合率。可见，Pb胁迫下，不同茶树的解毒方式存在差异。

关键词: 茶树, 铅胁迫, 生理特性, 亚细胞, 分布特征