Abstract: The indigenous bacterial community structures of seeds harvested in 2 consecutive years from 2 northern rice varieties ‘Yuefu’ and ‘Jindao 305’ with different disease-resistance were studied via establishing bacteria 16S rRNA gene clone library. In 2008, 211 and 174 positive clones, 22 and 25 OTUs were gained from library of ‘Yuefu’ and ‘Jindao 305’, and in 2009, 164 and 137 positive clones, 11 and 19 OTUs were obtained. Four bacteria groups were detected in these 2 varieties: α-Proteobacteria, β-Proteobacteria, γ-Proteobacteria and Actinobacteria. Among them α-Proteobacteria and γ-Proteobacteria were dominant groups. It was discovered that more abundant genera, and more stable community structure of seed indigenous bacteria was harbored in ‘Jindao 305’, which had stronger disease-resistance than ‘Yuefu’.

Key words: rice seed, disease resistance, indigenous bacterial community, diversity, 16S rRNA gene clone library

摘要： 通过构建细菌16S rRNA基因克隆文库，对相同生长环境中两个抗病性不同的北方水稻品种“越富”和“津稻305”，连续两年跟踪研究了其种子的固有细菌群落结构，2008年从文库“越富”和“津稻305”中分别得到211个和174个阳性克隆，22个和25个OTUs，2009年分别得到164个和137个阳性克隆，11个和19个OTUs。检测到固有细菌分属于α-Proteobacteria、β-Proteobacteria、γ-Proteobacteria和Actinobacteria，其中α-Proteobacteria和γ-Proteobacteria均为优势类群。并发现抗病性较强的“津稻305”种子中固有细菌的多样性比“越富”丰富，且群落结构比较稳定。

关键词: 水稻种子, 抗病性, 固有细菌群落, 多样性, 16S rRNA基因克隆文库