Abstract:

To understand the structure and diversity of fungal and bacterial communities in root tissues and rhizosphere soil of Lycium barbarum plants infested with root rot， root tissues and rhizosphere soil samples of Lycium barbarum infested with root rot in Qaidam region were analyzed using high-throughput sequencing technology. The results showed that bacterial species were slightly more abundant in soil than that in root tissue， and fungal diversity and abundance were not significantly different between soil and root tissue samples. At phylum level， Proteobacteria and Bacteroidetes had higher abundance in bacterial communities， and Ascomycota and Basidiomycota had higher abundance in fungal communities. At genus level， Pseudomonas was dominated in bacteria， and Cladosporium and Mortierella were dominated in fungi. The correlation analysis showed that bacteria were more different between root tissue and soil samples， and fungi were better correlated in root tissue and soil samples. Both Cladosporium and Mortierella were significantly upregulated in the root system and soil， which assumed to be associated with the pathogenesis of root rot of Lycium barbarum.

Key words: Lycium barbarum, root rot, high-throughput sequencing, microbial diversity

摘要：

为了解枸杞受根腐病菌侵染后植株根组织与根际土壤中真菌与细菌群落结构以及多样性，采用高通量测序技术对柴达木地区患根腐病枸杞的根组织和根际土壤样本进行分析。结果表明，根际土壤中的细菌群落丰度略高于根组织，而真菌多样性和丰度在根际土壤和根组织样本中无显著差异。在门水平，细菌群落中变形菌门（Proteobacteria）和拟杆菌门（Bacteroidetes）的相对丰度较高，真菌群落中子囊菌门（Ascomycota）和担子菌门（Basidiomycota）的相对丰度较高；在属水平，细菌中以假单胞菌（Pseudomonas）为主，真菌中以枝孢属（Cladosporium）和被孢霉属（Mortierella）为主。相关性分析显示，细菌群落在根组织和根际土壤样本中差异较大，而真菌群落在根组织和根际土壤样本间相关性较高；枝孢属（Cladosporium）和被孢霉属（Mortierella）的相对丰度在根组织和根际土壤中均显著上调，初步推测这2属真菌可能与枸杞根腐病发病相关。

关键词: 枸杞, 根腐病, 高通量测序, 微生物多样性