OsAFC2 Involved in the Regulation of mRNA Alternative Splicing in Oryza sativa

doi:10.13304/j.nykjdb.2015.271

Journal of Agricultural Science and Technology ›› 2016, Vol. 18 ›› Issue (1): 32-39.DOI: 10.13304/j.nykjdb.2015.271

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OsAFC2 Involved in the Regulation of mRNA Alternative Splicing in Oryza sativa

LIANG Meng, ZHANG Yan, XU Miao-yun, ZHANG Lan, WANG Lei*

(Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

Received:2015-05-13 Revised:2015-07-22 Online:2016-02-15 Published:2016-02-15

水稻基因OsAFC2参与mRNA可变剪切调控

梁蒙,张艳,徐妙云,张兰,王磊*

(中国农业科学院生物技术研究所, 北京 100081)

通讯作者: 王磊，研究员，博士，主要从事植物分子生物学与基因工程的研究。E-mail：wanglei01@caas.cn
作者简介:梁蒙|硕士研究生|研究方向为植物分子生物学与基因工程。E-mail：liangmeng666@126.com。
基金资助:
国家自然科学基金项目（31271801）资助。

Abstract

Abstract:

AFC2 plays an important role in the regulation of mRNA alternative splicing by phosphylating SR proteins. Up to now, the regulatory function of rice AFC2 has not been analyzed. In this study, a mutant of OsAFC2 gene was obtained by screening rice RNAi library. Further studies showed that OsAFC2 located in the nucleus and expressed in the rice various tissue, of which, OsAFC2 was highly expressed in rice leaves and low expressed in rice stem. Compared with the wild type control, the expression of OsAFC2 was obviously downregulated in the RNAi mutant and upregulated in overexpression plants. RNA-seq analysis found that the number of the alternative splicing transcripts significantly increased in OsAFC2 RNAi plants, indicating OsAFC2 involved in the regulation of mRNA alternative splicing in the nucleus.

Key words: Oryza sativa, OsAFC2, mRNA, alternative splicing

摘要：

mRNA的可变剪切受到SR蛋白磷酸化水平的精确调控，AFC2作为SR蛋白激酶，能够影响mRNA可变剪切。在水稻RNAi突变体库中发现一个OsAFC2基因沉默突变体，进一步研究显示该基因定位于细胞核内，在水稻的各个组织部位均有表达，其中在叶片中表达量较高，在茎中表达量较低。与对照相比，在OsAFC2抑制表达的植株中，检测到的可变剪切数量明显增加，表明OsAFC2参与水稻细胞核中mRNA可变剪切的调控。

关键词: 水稻, OsAFC2, mRNA, 可变剪切

CLC Number:

Q786

LIANG Meng, ZHANG Yan, XU Miao-yun, ZHANG Lan, WANG Lei*. OsAFC2 Involved in the Regulation of mRNA Alternative Splicing in Oryza sativa[J]. Journal of Agricultural Science and Technology, 2016, 18(1): 32-39.

梁蒙,张艳,徐妙云,张兰,王磊*. 水稻基因OsAFC2参与mRNA可变剪切调控[J]. 中国农业科技导报, 2016, 18(1): 32-39.

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OsAFC2 Involved in the Regulation of mRNA Alternative Splicing in Oryza sativa

水稻基因OsAFC2参与mRNA可变剪切调控

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