Abstract: Farmland application and backwash of pig house are the main ways to utilize  swine manure based digested slurry from swine manure. However, the potential risk of pathogenic microorganisms exists in digested slurry produced by psychrophilic and mesophilic biogas digesters. Referring to the pasteurization technology after anaerobic fermentation in the European Union, the heat treatment disinfection method was used to disinfect the digested slurry from swine manure. In order to optimize heat treatment disinfection parameters of digested slurry, the number of coliform bacteria and fecal coliform were used as indicators to evaluate the key factors and disinfection effects of heat treatment. Based on the preliminary test of heating temperature of 50～80 ℃, the experiments were carried out using digested slurry with 3 total solids (TS) content (2%, 4%, 6%), 4 heating temperatures (525, 55, 575, 60 ℃), 3 heating time (the time from 35 ℃ to heating temperature, 15 min, 30 min), respectively. The Results showed that the heating temperature and heating time had an significant influence on the disinfection efficiency of swine manure digested slurry (P< 001), while the TS of digested slurry had no significant effect (P>005). In addition, the temperature growth rate of digested slurry decreased with the increase of total solids content in digested slurry. For digested slurry from swine manure used in this study, the average group number of coliform and fecal coliform in digested slurry were less than 1 CFU·mL-1 and 003 MPN·mL-1 after continuous heating time for 15 min at 60 ℃, respectively, which could meet the requirements of reclaimed water quality standard (SL368-2006) for pathogenic microorganisms disinfection in the reuse of reclaimed water for agriculture. The present research provided a reference for the development of disinfection technology of digested slurry from swine manure.

Key words: digested slurry, heat treatment, disinfection, temperature, time

摘要： 农田利用和回冲圈舍是猪场沼液资源利用的主要途径，然而常温或中温厌氧发酵产生的猪场沼液有存在病原微生物的风险。借鉴欧盟厌氧发酵后巴氏消毒技术，采用热处理消毒法对猪场沼气工程厌氧发酵沼液进行消毒试验研究，为优化沼液热处理消毒的参数，以大肠菌群、粪大肠菌群的数量为指标，在进行50～80 ℃预备试验基础上，采用3种总固体含量（2%、4%、6%）的沼液、在4种加热温度（525、55、575、60 ℃）、3种加热时间（35 ℃升温至加热温度时间、15 min、30 min）的条件下，探讨猪场沼液热处理消毒的影响因素及其消毒效果。研究表明，加热温度和加热时间对沼液热处理消毒效果均有极显著影响（P＜001），沼液总固体含量对沼液热处理消毒效果无显著影响（P＞005），但沼液总固体含量越大，沼液升温速率越慢。对于本研究所用厌氧发酵沼液，在加热温度60 ℃，持续加热15 min后，沼液中大肠菌群数平均小于1 CFU·mL-1、粪大肠菌群数平均小于003 MPN·mL-1，满足《再生水水质标准》(SL3682006)再生水回用于农业病原微生物杀灭要求。研究可为猪场沼液消毒处理技术开发提供参考依据。

关键词: 沼液, 热处理, 消毒, 温度, 时间