Abstract:

An efficient Liquid chromatography-fluorescence detection method for determination of nine kinds of quinolones in compound premix was established.The method is simple,rapid,accurate,sensitive and reproducible.The concentration of nine kinds of quinolines and peak area was linear correlation between 0.05~4 μg/mL,correlation coefficient r was above 0.999 9.Average reclaim ratio was above 80% and coefficient of variation was not exceeding 7%.

Key words: quinolones, HPLC, fluorescence detection, chromatographic conditions;

摘要：

建立了以高效液相色谱-荧光检测法同时测定复合预混合饲料中9种喹诺酮类药物的最佳方法。该法简便、快速、准确、灵敏度高、重现性好。9种喹诺酮类药物在浓度0.05~4μg/mL范围内与峰面积呈线性相关,相关系数r>0.999 9,平均回收率均在80%以上,RSD均在7%以内。

关键词: 喹诺酮类 高效液相色谱 荧光检测法 色谱条件