Abstract:

Expression and functions analysis of ABCD genes, which related to the development of floral organ in rape （Brassica napus ）, was carried out by optimized semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR） with the buds and flowers of rape at seven different development stagges as the material, which was the first time to study ABCD model in rape. It indicated that in rape, six of ABCD genes that are Apetalal, Apetala2, Apetala3, Pistillata, Agamous and Agamous-like11, exhibited a variation in the expression level at the different development stages. This paper also expounds the similarity and differences of ABCD model in gene expression and expression level in both Arabidopsis and rape. It is of great significance for further analyzing the expression of ABCD gene in buds and flowers, for rape breeding, and for research on cytoplasmic male sterile and on flower pattern change.

Key words: rape, ABCD genes , gene expression , semi-quantitative RT-PCR

摘要：

以油菜处于花蕾发育过程7个生长期的花蕾和花朵为材料，利用优化的半定量RT—PCR技术在转录水平对ABCD模型的6个功能基因，即Apetalal、Apetala2、Apetala3、Pistillata、Agamous、Agamous—likell，进行分析，探讨了油菜花蕾发育及开花过程中6个功能基因表达的差异。结果表明，在油菜花蕾发育及开花过程中ABCD模型中的6个功能基因的表达水平均有变化。研究阐述了ABCD模型在油菜与拟南芥中存在基因表达时期和表达水平相似性和差异，对进一步分析ABCD功能基因在油菜花蕾和花朵中的表达，油菜育种，雄性不育及花型改变的研究具有重要的意义。

关键词: 油菜 ABCD功能基因 基因表达 半定量RT—PCR