Abstract:

Cold-adapted enzymes are the enzymes which are thermolabile and have high catalytic efficiency at low temperatures. In the past 20 years, cold-adapted enzymes have been extensively studied. Because of the uniqueness of their structure and function, they become good materials to study protein structure and function. And studies on the structure and function of cold-adapted enzymes are becoming more and more detailed with time. In this review, characte ristics of the enzymatics, dynatics and thermodynatics of cold-adapted enzymes, the relationship between their structure and activity and the relationship between their stability and flexibility were analyzed.

Key words: cold-adapted enzymes, structure, activity, flexibility, stability

摘要：

适冷酶是指在低温下具有较高的催化效率，对热相对不稳定的酶。近20年来，适冷酶得到广泛的研究。由于适冷酶结构和功能的特殊性，成为研究蛋白质结构和功能的好材料，其结构和功能的研究也越来越深入。对适冷酶的酶学、动力学和热力学特征，以及适冷酶结构与其活性、稳定性和柔性的关系进行了分析。

关键词: 适冷酶 结构 活性 柔性 稳定性