Abstract:

In this study, we studied on the pertinence between PnAG1 gene which relevant to NBS-LRR with drought stress, we compared the changes in peanut main defense enzyme activity and the dynamics of PnAG1 gene expression under different degrees of drought stress. After drought stress, leaf peroxidase (POD) activity, Catalase (CAT) activity, superoxide dismutase (SOD) activity, glutathione oxidoreductase (GR) activity, malondialdehyde (MDA) content and PnAG1 Gene expression change dynamic of 2 peanut varieties during different periods were measured. The results showed that after drought stress the changes in POD、GR、MDA of 2 varieties showed a tendency of first rising then declined. The SOD activity was rising continuously, while the changes in  PnAG1 gene expression also showed a trend of first decline then rise, similar to the changes in defensive enzyme activity. Therefore, conclusion was drew that the PnAG1 genes might be interrelated with the plant defense system on the basis of the disease resistance.

Key words: peanut, Real-time PCR, enzyme activity determination, NBS-LRR, drought stress

摘要：

为了探讨NBS-LRR结构域中抗性相关基因PnAG1与干旱胁迫的相关性，比较花生在不同干旱程度胁迫下主要防御酶活性变化情况以及PnAG1基因表达量变化动态。经干旱胁迫后测定不同时期两花生品种叶片的过氧化物酶（POD）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽还原酶（GR）活性、丙二醛（MDA）含量和PnAG1基因表达量的动态变化。结果表明，在干旱胁迫后两品种的POD、GR、MDA变化均表现出先下降后上升的趋势，SOD活性则为表现为持续上升，而PnAG1基因的表达量变化同样也呈现出先下降后上升的趋势，与防御酶活性变化相似，因此推断PnAG1基因在抗病基础之上可能同植物防御体系存在相关。

关键词: 花生;荧光定量PCR;酶活测定;NBS-LRR;干旱胁迫