Abstract: The pseudo-response regulator (PRR) proteins  are important part of the central oscillator of plant biological clocks. PRRs participate in the regulation of plant circadian rhythm. In addition, PRRs also play important roles in growth, development and stress adaptation. In this study, the PRR family members were identified in licorice (Glycyrrhiza uralensis Fisch.) through bioinformatics methods, and its expression patterns were analyzed under salt, low phosphorus and drought stresses in virtue of transcriptome data. The results showed that: a total of seven GurPRR members were identified in the licorice plant genome and divided into three clades based on the phylogenetic results. Moreover, numerous cold-, drought-, light-, methyl jasmonate-, abscisic acid- and salicylic acid-responsive cis-elements were observed in the GuPRR promoter regions. Transcriptome data showed that the expressions patterns of PRR family genes in Glycyrrhiza uralensis were different in different tissues and responded to different stresses. The results provide abundant information about PRR family genes in Glycyrrhiza uralensis that would benefit for  further understanding of functional roles in licorice plants.

Key words: Glycyrrhiza uralensis, PRR gene family, bioinformatics analysis, gene expression

摘要： 伪应答调控（PRR）蛋白作为植物生物钟中央振荡器的重要组成部分，参与植物昼夜节律的调节，在植物生长发育、逆境胁迫响应等生命活动中发挥重要作用。利用生物信息学方法鉴定了甘草PRR蛋白编码基因，并结合转录组数据分析其在盐胁迫、干旱胁迫和低磷胁迫下的表达模式。结果表明：甘草基因组中共鉴定出7个GuPRR基因；根据系统发育进化分析将甘草PRR蛋白分为3个分支。在GuPRR启动子区还发现了大量的低温、干旱、光、茉莉酸甲酯、脱落酸和水杨酸响应的顺式元件。转录组数据分析结果表明，7个GuPRR基因在不同的组织都存在表达且响应不同的非生物胁迫。这些结果为甘草PRR基因家族的研究提供了理论的基础资料，将有助于深入了解甘草PRR基因家族的功能。

关键词: 甘草, PRR基因家族, 生物信息学分析, 基因表达