Abstract:

Taking rape pollen as raw material, by using pulsed electric field wall-breaking method, this paper studied the extraction effect of total flavones from rape pollen. On the basis of testing 3 single factors——electric field intensity, pulses number and backflow temperature, taking total flavones extraction rate as response value, the best extraction technology for total flavones were optimized by Box-Behnken method. The results showed that electric field intensity had a significant effect on rape pollen cell wall breaking. Under the optimal extraction technology condition, i.e. electric field intensity of 28 kV/cm, pulses number of 508 and backflow temperature of 82℃, the total flavones extraction rate could reach 4.508%.

Key words: rape pollen, total flavones, pulsed electric field, response surface methodology, extraction

摘要：

以油菜花粉为原料，利用脉冲电场破壁法，探讨了油菜花粉中黄酮类化合物的提取工艺。在脉冲电场强度、脉冲数、回流温度3个单因素试验的基础上，以总黄 酮类物质提取率为响应值，采用Box-Behnken响应面法设计优化了总黄酮类化合物提取的最佳工艺条件。结果表明电场强度严重影响着花粉细胞的破壁效果，且 在最优的提取工艺条件下，即电场强度28 kV/cm，脉冲数508，回流温度82℃，油菜花粉黄酮类物质提取率高达4.508%。

关键词: 油菜花粉, 总黄酮, 脉冲电场, 响应面法, 提取