Abstract: In order to investigate the effect of bacterium Bacillus amyloliquefaciens on growth performance, digestive enzymes activities, serum proteins levels and liver superoxide dismutase (SOD) activity in juvenile black seabream (Sparus macrocephalus), this paper reared the fish with 7 g body weight in a small cement pool of an indoor micro-water circulation system and fed diets containing Bacillus amyloliquefaciens at a dose of 0, 0.025%, 0.05%, and 0.1%, respectively. After 56 d, the results showed that the bacterium led to improve weight gain rate (WGR) and specific growth rate (SGR) in juvenile black seabream. The WGR and SGR were significantly higher in juvenile black seabream fed by diet supplemented with 0.05% of Bacillus amyloliquefaciens than that of the fish fed by control diet. The intestinal protease and lipase activities in the fish fed by diet supplemented with Bacillus amyloliquefaciens were significantly higher than that of the control group. The total serum protein levels of juveniles fed with 0.025% Bacillus dietary supplement were significantly higher than that of the control group, among them the globulin and albumin levels were extremely increased. In addition, the intestinal amylase activity and hepatic SOD activity were also obviously higher in the 0.1% Bacillus amyloliquefaciens dietary supplement group than that of the control. These results indicated that optimal dose of Bacillus amyloliquefaciens administration could increase the intestinal digestive enzyme activity, improve antioxidant ability in juvenile black seabream, and promote their growth performance.

Key words: Bacillus amyloliquefaciens, juvenile black seabream, growth promotion, antioxidant

摘要： 为探讨解淀粉芽孢杆菌对黑鲷幼鱼的生长、消化酶活性、血清蛋白水平以及肝脏超氧化物歧化酶活性的影响，在水温（28±1）℃下，将体质量7 g的黑鲷(Sparus macrocephalus)幼鱼室内微流水小型水泥网箱池中，分别投喂添加了0、0.025%、0.05%和0.1%解淀粉芽孢杆菌的饲料。56 d的饲养结果表明：0.05%解淀粉芽孢杆菌组黑鲷幼鱼的增重率和特定生长率显著高于对照组；摄食含解淀粉芽孢杆菌饲料的黑鲷幼鱼肠道蛋白酶和脂肪酶活性均极显著高于对照组；0.025%解淀粉芽孢杆菌组幼鱼血清总蛋白水平极显著高于对照组，其中球蛋白和白蛋白水平极显著升高；0.1%解淀粉芽孢杆菌组黑鲷幼鱼肠道中淀粉酶、肝脏中超氧化物歧化酶活性显著高于对照组。研究结果表明在饲料中添加适量的解淀粉芽孢杆菌能显著提高黑鲷幼鱼肠道消化酶活性，提高抗氧化能力，促进生长。

关键词: 解淀粉芽孢杆菌, 黑鲷, 促生长, 抗氧化