Abstract: To explore the content of glycerol in honey under different conditions, this experiment determined the content of glycerol by glycerol enzyme method. The results showed that the glycerol enzyme preparation method could be used to determine the content of glycerol in honey. The recovery rate of oil content in this experiment was 95.7%~115.0%. The glycerol content of RSD was 2%~7% in the precision test and the glycerol content of RSD was 1%~5% in the repeatability test. In addition, the results was independent of pH when its level varied in the range of 3.00~7.00. Moreover, the content of glycerol in honey presented tiny variation by this method within 7 d. The content of glycerol in honey was stable under 4~25℃. Thus, this method could be used to determine the content of glycerol in different honey, and meet the requirements of basic determination in practical production.

Key words: honey, enzymic preparation, glycerol

摘要： 为了探究在不同条件下蜂蜜中的甘油含量，通过甘油酶制剂法测定其含量，实验结果表明，利用甘油酶制剂法能较稳定的测定蜂蜜中的甘油含量，其加标回收率实验的回收率在95.7%~115.0%之间，精密度实验甘油含量的RSD在2%~7%，重复性实验甘油含量的RSD在1%~5%；同时pH为3.00~7.00时，蜂蜜中的甘油含量变化较稳定，在7 d内，通过此法测定蜂蜜中甘油的含量无明显变化，在4~25℃条件下，蜂蜜中甘油含量变化较稳定。同时采用此法对不同蜂蜜中的甘油含量进行测定，能够达到生产上基本测定的要求。

关键词: 蜂蜜, 酶制剂, 甘油