关键词: 低氮胁迫, 小麦, 叶片, 转录组分析

Abstract: Nitrogen is the necessary element for wheat growth. It is important to identify genes involved in the control of regulatory network of low-nitrogen stress in wheat through transcription analysis, which has great significance for revealing the mechanism of low nitrogen tolerance and promoting wheat breeding. In this study, Illumina HiseqTM 2500 high-throughput sequencing technology was used to sequence the transcriptome of leaves from Jinmai 47 under normal and low nitrogen conditions. Differentially expressed genes (DEG) were screened and low nitrogen response related genes were analyzed by using the GO and KEGG database for annotation in wheat. The results showed that 52 383 726 and 52 192 061 sequences were obtained from the control and the treatment group, respectively. 1 267 DEG were identified, in which 179 were up-regulated and 1 088 were down-regulated. The GO analysis of DEG showed that 44 functional groups of three categories were identified. According to KEGG metabolic pathway, these genes were assigned to 178 signaling pathways, in which amino acid metabolism, carbohydrate metabolism, lipid metabolism, signal transduction and immune system were mostly enriched. Transcription factor analysis showed that the family of WRKY, MYB and NAC displayed the most significant changes in gene expression under low nitrogen conditions.

Key words: low nitrogen stress, wheat, leaf, transcriptome analyses