关键词: 斜纹夜蛾, 核型多角体病毒Ⅱ型, ORF117, 转录时相分析, 表达时相分析

Abstract:

The structure and function of Spodoptera litura multicapsid nucleopolyhedrovirus Ⅱ(SpltMNPVⅡ) ORF117 gene were studied. The gene and protein sequences of ORF117 (coden protein GP117) were analyzed by bioinformatics methods. The promoter activities and the time course of mRNA transcription were analyzed. Part of GP117 protein was expressed and purified to preparing the polyclonal antibody. The expression phase of ORF117 and cellular location of GP117 were analyzed using the antibody. Sequence analysis demonstrated that this gene has a 1 203 bp ORF, encoding 400 amino acids with a predicted molecular weight of 45.5 kDa and a predicted isoelectric point of 5.06. Both promoter activities analysis and the time course of mRNA transcription showed that ORF117 was a late gene. Western blotting was used to detected the gene ORF117′s expression phase and the result identified with the transcription phase. The result of cellular localization of ORF117 showed that the GP117 existed in cytoplasm more than nucleus.

Key words: Spodoptera litura, multicapsid nucleopolyhedrovirusⅡ, ORF117, transcription phase analysis, expression phase analysis