外源钙诱导植物应答镉胁迫的效应及作用机制研究进展

doi:10.13304/j.nykjdb.2024.0223

摘要/Abstract

摘要：

镉是中国最严重的土壤污染物之一，可以通过根系吸收或叶面吸收而在植物体内积累，进而影响植物各种生理生化功能，引起生理代谢紊乱和生长受阻。钙在植物的生长发育及其对环境信号的响应中起重要作用。综述了外源钙对植物体内镉的积累与分布、生长发育、光合作用、抗氧化系统、营养物质、形态指标等的影响，并阐述了钙信号诱导植物应答镉胁迫的作用机制，以期为植物生态修复镉污染土壤提供参考。

关键词: 钙, 镉, 生理生化, 应答机制

Abstract:

Cadmium （Cd） is one of the most serious soil pollutants in China. It can accumulate in plants through absorption by roots or leaves， leading to interference in various physiological and biochemical functions， causing disruption of plant physiological metabolism and growth inhibition. Calcium （Ca） plays an important role in the growth and development of plants and the response to environmental signals. The effects of exogenous Ca on the Cd accumulation and distribution， growth and development， photosynthesis， antioxidant system， nutrients and morphological indicators of the plants were summaried， and the mechanism of Ca signaling induced plant response to Cd stress was discussed， which provided reference for plant ecological remediation of Cd-contaminated soil.

Key words: calcium, cadmium, physiology and biochemistry, response mechanism

中图分类号:

Q945.78

黄焰新, 吴香, 曹艳, 闫旭宇, 李玲. 外源钙诱导植物应答镉胁迫的效应及作用机制研究进展[J]. 中国农业科技导报, 2025, 27(9): 165-171.

Yanxin HUANG, Xiang WU, Yan CAO, Xuyu YAN, Ling LI. Research Progress on Effect and Mechanism of Exogenous Calcium Induced Plant Response to Cadmium Stress[J]. Journal of Agricultural Science and Technology, 2025, 27(9): 165-171.

参考文献 57

[1]	PESTANA M, VARENNES D A, ABADÍA J, et al.. Differential tolerance to iron deficiency of citrus rootstocks grown in nutrient solution [J]. Sci. Hortic., 2005, 104(1): 25-36.
[2]	MEDVEDEV S S.Calcium signaling system in plants [J]. Russ.J. Plant Physiol., 2005,52(2):249-270.
[3]	PIRAYESH N, GIRIDHAR M, KHEDHER A B, et al.. Organellar calcium signaling in plants: an update [J/OL]. Biochim. Biophys. Acta Mol. Cell. Res., 2021, 1868 (4):118948[2023-02-20]. .
[4]	郑远,陈兆进.植物细胞器钙信号研究进展[J].植物生理学报,2015,51(8):1195-1203.
	ZHENG Y, CHEN Z J.Organellar calcium signaling in plants [J]. Plant Physiol. J., 2015,51(8):1195-1203.
[5]	WANG L, CUI X F, CHENG H G,et al..A review of soil cadmium contamination in China including a health risk assessment [J]. Environ.Sci.Pollut.Res., 2015,22(21):16441-16452.
[6]	ALTAF M A,NAZ S, KUMAR R,et al.. Unraveling the mechanisms of cadmium toxicity in horticultural plants:implications for plant health [J]. South African J. Bot., 2023,163: 433-442.
[7]	RASAFI T E, OUKARROUM A, HADDIOUI A, et al.. Cadmium stress in plants:a critical review of the effects, mechanisms, and tolerance strategies [J]. Crit. Rev. Environ. Sci. Technol., 2022, 52(5): 675-726.
[8]	张亚利,王继先.镉对钙代谢的影响及相关机制研究进展[J].环境与健康杂志,2004,21(4):269-271.
	ZHANG Y L, WANG J X.Advances in effects of cadmium on calcium metabolism and its associated potential mechanisms [J]. J. Environ. Health, 2004,21(4):269-271.
[9]	李海燕. 钙对土壤镉化学形态及水稻吸收镉的影响[D]. 贵阳: 贵州师范大学, 2022.
	LI H Y. Effects of calcium on chemical speciation of cadmium in the soil and cadmium absorbed by rice [D]. Guiyang: Guizhou Normal University, 2022.
[10]	LUBYANOVA A R, BEZRUKOVA M V, SHAKIROVA F M.Interaction between signal pathways upon formation of plant defense in response to environmental stress factors [J]. Russ. J.Plant Physiol., 2021,68(6):989-1002.
[11]	CHENG Y W, WANG N, LIU R X,et al..Cinnamaldehyde facilitates cadmium tolerance by modulating Ca²⁺ in Brassica rapa [J/OL]. Water Air Soil Pollut.,2021,232(1):19 [2024-02-20]. .
[12]	李娜,李涛,梅馨月,等. 2种外源钙对镉胁迫三七生理生化特性及其镉积累的影响[J].贵州农业科学,2019,47(7):131-137.
	LI N, LI T, MEI X Y, et al.. Effects of two exogenous calcium on physiological and biochemical characteristics and cadmium accumulation of Panax notoginseng of cadmium stress [J].Guizhou Agric. Sci., 2019,47(7):131-137.
[13]	严勋. 镉对不同基因型水稻根系毒害效应及钙调控作用的研究[D]. 成都: 四川农业大学, 2019.
	YAN X. Toxicity of cadmium on roots of different genotypes of rice and regulation of calcium [D]. Chengdu: Sichuan Agricultural University, 2019.
[14]	杨训. 钙对茶树吸收Cd的阻控及其机理[D]. 合肥: 安徽农业大学, 2018.
	YANG X. Control of calcium on Cd absorption and its mechanism in tea plants [D]. Hefei: Anhui Agricultural University, 2018.
[15]	MAZHAR M W, ISHTIAQ M, MAQBOOL M, et al.. Synergistic application of calcium oxide nanoparticles and farmyard manure induces cadmium tolerance in mung bean (Vigna radiata L.) by influencing physiological and biochemical parameters [J/OL]. PLoS One,2023,18(3):e0282531 [2024-02-20]. .
[16]	赵素贞.Ca对秋茄Cd耐受性影响的研究[D].厦门:厦门大学,2014.
	ZHAO S Z. The role of calcium in cadmium tolerance of Kandelia obovata (S., L.) Yong [D]. Xiamen: Xiamen University, 2014.
[17]	唐琦.钙离子对水稻镉离子吸收转运特性的影响[D].哈尔滨:东北农业大学,2019.
	TANG Q. Effect of calcium on the characteristics of cadmium absorption in rice [D]. Harbin: Northeast Agricultural University, 2019.
[18]	YE W L, WU F, ZHANG G Y,et al..Calcium decreases cadmium concentration in root but facilitates cadmium translocation from root to shoot in rice [J]. J. Plant Growth Regul., 2020,39(1):422-429.
[19]	李乐乐, 李中阳, 吴大付, 等. 外源物质对镉胁迫下不同品种冬小麦苗期镉吸收特征的影响[J]. 灌溉排水学报, 2021, 40(1): 79-90.
	LI L L, LI Z Y, WU D F, et al.. Cadmium accumulation in wheat of different varieties at seedling stage as impacted by addition of exogenous elements [J]. J. Irrig. Drai., 2021, 40(1): 79-90.
[20]	WAHEED S, AHMAD R, IRSHAD M,et al..Ca₂SiO₄ chemigation reduces cadmium localization in the subcellular leaf fractions of spinach (Spinacia oleracea L.) under cadmium stress [J/OL]. Ecotoxcol. Environ. Saf., 2021,207:111230 [2024-02-20]. .
[21]	李路. 锌对镉胁迫下青稞幼苗生长及锌镉含量的影响[J]. 农业与技术, 2024, 44(1): 5-8.
	LI L. Effects of Zinc on growth and content of zinc and cadmium in highland barley seedlings under cadmium stress [J]. Agric. Technol., 2024, 44(1): 5-8.
[22]	刘佳丽. 重金属镉对大豆种子萌发与幼苗生长的影响[J]. 农业技术与装备, 2023( 12):13-15.
	LIU J L. Effects of heavy metal cadmium on seed germination and seedling growth of soybean [J]. Agric. Technol. Equip., 2023(12): 13-15.
[23]	周高婷,杜剑波.硫酸盐还原菌WH16-1对烟草吸收镉的影响[J].工业微生物,2023,53(6):170-174.
	ZHOU G T, DU J B. The effectiveness of Cd content of tobacco in Cd-contaminated tobacco ssoil by sulfate-reducing bacterium WH16-1 [J]. Ind.Microbiol., 2023,53(6):170-174.
[24]	张丽辉,吴桑娜,孙奇,等.钙对铅胁迫下红花种子萌发和幼苗生长的影响[J].种子科技,2015,33(12):45-48.
[25]	周敏,徐小蓉.不同钙源浸种对益母草种子萌发的影响[J].科技咨询导报,2007,4(13):254,256.
[26]	韦小丽, 廖明. 钙浸种对马尾松种子发芽的影响[J]. 种子, 2005, 24(4): 34-36.
	WEI X L, LIAO M. Effect of calcium-soaking on germination of pine massoniana seed [J]. Seed, 2005,24(4): 34-36.
[27]	段咏新, 宋松泉, 傅家瑞. 钙在黄皮种子萌发中的作用[J]. 种子, 1997(2): 4-6.
	DUAN Y X, SONG S Q, FU J R. Effects of calcium on germination of Chinese wampee seeds [J]. Seed, 1997(2): 4-6.
[28]	辛慧慧.棉花对低温胁迫的生理响应及调控研究[D].石河子: 石河子大学,2015.
	XIN H H. The research on the effect of low temperature stress on physiological responses and regulations of cotton [D]. Shihezi: Shihezi University, 2015.
[29]	LIU J L, FENG X Y, QIU G Y, et al.. Inhibition roles of calcium in cadmium uptake and translocation in rice:a review [J/OL]. Int. J. Mol. Sci., 2023,24(14):11587 [2024-02-20]..
[30]	SAKOUHI L, RAHOUI S, MASSOUD MBEN, et al.. Calcium and EGTA alleviate cadmium toxicity in germinating chickpea seeds [J]. J. Plant Growth Regul., 2016,35(4):1064-1073.
[31]	李孟盈, 胡龙兴, 向佐湘. 镉胁迫对南方苋种子萌发及幼苗生长和生理的影响[J].草地学报, 2024,32(3):754-762.
	LI M Y, HU L X, XIANG Z X. Effect of cadmium on the germination, growth and physiology of seedlings of amaranthus australis [J]. Acta Agrestia Sin., 2024,32(3):754-762.
[32]	李晓宇, 杨成超, 李文颖, 等. 不同杨树品种苗期对镉胁迫的差异性分析[J]. 中南林业科技大学学报, 2024, 44(3): 11-21.
	LI X Y, YANG C C, LI W Y, et al.. Differential responses of poplar cultivars to cadmium stress at seedling stage [J]. J. Central South Sci-Technol. Univ. For., 2024, 44(3): 11-21
[33]	肖才升,谢心,李锋,等.锰处理下棉花幼苗根部对镉胁迫的生理响应[J].华北农学报,2023,38(6):55-61.
	XIAO C S, XIE X, LI F, et al.. Physiological response of two cotton seedlings to cadmium stress treated with different concentrations of manganese [J]. Acta Agric. Boreali-Sin., 2023,38(6):55-61.
[34]	姜倩倩,林家燕,杨槟瑜.钙对镉胁迫下平邑甜茶根系活性氧生成和细胞损伤的影响[J].潍坊学院学报,2019,19(6):23-28.
	JIANG Q Q, LIN J Y, YANG B Y. The role of calcium in the generation of reactive oxygen species and cell damage in roots of Malus hupehensis Rehd.under cadmium stress [J]. J. Weifang Univ., 2019,19(6):23-28.
[35]	刘伟, 樊文华, 刘奋武, 等. 施磷对镉胁迫下黄瓜苗期光合作用及抗氧化酶系统的影响[J]. 土壤通报, 2022, 53(3): 596-604.
	LIU W, FAN W H, LIU F W, et al.. Effects of phosphorus on photosynthesis and antioxidant enzyme system of cucumber seedlings under cadmium stress [J]. Chin. J. Soil Sci., 2022, 53(3): 596-604.
[36]	HASHEM A, ALLAH E FABD, ALQARAWI A A, et al.. Role of calcium in AMF-mediated alleviation of the adverse impacts of cadmium stress in Bassia indica [Wight] A. J. Scott [J]. Saudi. J. Biol. Sci., 2019, 26(4):828-838.
[37]	张丽. 外源施加钙、一氧化氮对镉胁迫下旱柳抗氧化系统活性及相关基因表达的影响[D]. 沈阳: 辽宁大学, 2019.
	ZHANG L. Effects of exogenous application calcium and nitric oxide on antioxidant system activity and expression of related genes in salix matsudana koidz. under cadmium stress [D]. Shenyang: Liaoning University, 2019.
[38]	ALLAH F E ABD, HASHEM A, ALQARAWI A A, et al.. Calcium application enhances growth and alleviates the damaging effects induced by Cd stress in sesame (Sesamum indicum L.) [J]. J. Plant Interact, 2017, 12(1): 237-243.
[39]	POOVAIAH B W.Biochemical and molecular aspects of calcium action [J]. Acta Hortic., 1993,326:139-148.
[40]	ARORA R, PALTA J P. In vivo perturbation of membrane-associated calcium by freeze-thaw stress in onion bulb cells [J]. Plant Physiol., 1988,87(3):622-628.
[41]	赵素贞,洪华龙,严重玲.钙对镉胁迫下秋茄叶片光合作用及超微结构的影响[J].厦门大学学报(自然科学版),2014,53(6):875-882.
	ZHAO S Z, HONG H L, YAN Z L. Effect of calcium supply on photosynthesis and ultrastructure of cells of Kandelia obovata (S.L.) yong under cadmium stress [J]. J. Xiamen Univ.(Nat. Sci.),2014,53(6):875-882.
[42]	TAMÁS L, BOČOVÁ B, HUTTOVÁ J, et al.. Depletion of extracellular calcium increases cadmium toxicity in barley root tip via enhanced Cd uptake-mediated superoxide generation and cell death [J/OL]. Acta Physiol.Plant., 2017,39(2):50 [2024-02-20]. .
[43]	王明霞,黄建国,袁玲,等.铝胁迫下外源钙对外生菌根真菌抗氧化保护酶活性的影响[J].环境科学,2012,33(10):3675-3679.
	WANG M X, HUANG J G, YUAN L, et al.. Effect of exogenous calcium on the activities of antioxidative protective enzymes in ectomycorrhizal fungi under aluminum stress [J]. Environ. Sci.,2012,33(10):3675-3679.
[44]	马惠芳,王进鑫,初江涛,等.水分和铅胁迫对槐树幼苗渗透调节物质的影响[J].西北农林科技大学学报(自然科学版),2011,39(9):101-106, 117.
	MA H F, WANG J X, CHU J T, et al.. Effects of water and lead stress on organic osmotic adjustment substance in the seedlings of two types of pagoda trees [J]. J.Northwest A&F Univ. (Nat. Sci.), 2011,39(9):101-106, 117.
[45]	丁艳菲,朱诚,王珊珊,等.植物microRNA对重金属胁迫响应的调控[J].生物化学与生物物理进展,2011,38(12):1106-1110.
	DING Y F, ZHU C, WANG S S, et al.. Regulation of heavy metal stress response by plant microRNAs [J]. Prog.Biochem.Biophys., 2011,38(12):1106-1110.
[46]	赵士诚. 镉诱导植物氧化代谢与钙信使的变化及分子机理[D]. 北京: 中国农业科学院, 2008.
	ZHAO S C. Cadmium-induced changes in oxidative metabolism and calcium messenger of plant and their molecular mechanisms [D]. Beijing: Chinese Academy of Agricultural Sciences, 2008.
[47]	SAKOUHI L, HUSSAAN M, MURATA Y, et al.. Role of calcium signaling in cadmium stress mitigation by indol-3-acetic acid and gibberellin in chickpea seedlings [J]. Environ.Sci. Pollut. Res., 2024,31(11):16972-16985.
[48]	Muhammad Mudassir Nazir. 钙和氧化钙纳米颗粒减缓大麦砷和镉毒害及其机理[D]. 杭州: 浙江大学,2022.
	NAZIR M M. Alleviation of arsenic and cadmium toxicity in barley by calcium and calcium oxide nanoparticles (CaO NPs) and their mechanisms [D]. Hangzhou: Zhejiang University, 2022.
[49]	CHOONG G, LIU Y, TEMPLETON D M. Interplay of calcium and cadmium in mediating cadmium toxicity [J]. Chem. Biol.Interact., 2014,211:54-65.
[50]	ZHANG Y, WANG Z, LIU Y, et al.. Plasma membrane-associated calcium signaling modulates cadmium transport [J]. New Phytol., 2023,238(1):313-331.
[51]	SHUMAYLA, TYAGI S, SHARMA Y, et al.. Expression of TaNCL2—a ameliorates cadmium toxicity by increasing calcium and enzymatic antioxidants activities in Arabidopsis [J/OL]. Chemosphere,2023,329:138636 [2024-02-20]. .
[52]	RIVETTA A, PESENTI M, SACCHI G A, et al.. Cadmium transport in maize root segments using a classical physiological approach:evidence of influx largely exceeding efflux in subapical regions [J/OL]. Plants,2023,12(5):992 [2024-02-20]. .
[53]	王宇, 郜耿东, 葛萌萌, 等. 钙在作物生长发育中的功能及应用[J].作物学报, 2024, 50(4):793-807.
	WANG Y, GAO G D, GE M M, et al.. Function and application of calcium in plantgrowth and development [J]. Acta Agron. Sin., 2024, 50(4):793-807.
[54]	赵士诚,孙静文,王秀斌,等.镉对玉米苗中钙调蛋白含量和Ca²⁺-ATPase活性的影响[J].植物营养与肥料学报,2008,14(2):264-271.
	ZHAO S C, SUN J W, WANG X B, et al.. Effects of cadmium on calmodulin content and Ca²⁺-ATPase activities of maize (Zea mays) seedling [J]. J. Plant Nutr.Fert., 2008,14(2):264-271.
[55]	TREESUBSUNTORN C, THIRAVETYAN P. Calcium acetate-induced reduction of cadmium accumulation in Oryza sativa:expression of auto-inhibited calcium-ATPase and cadmium transporters [J]. Plant Biol., 2019,21(5):862-872.
[56]	LI Z R, MEI X Y, LI T, et al.. Effects of calcium application on activities of membrane transporters in Panax notoginseng under cadmium stress [J/OL]. Chemosphere,2021,262:127905[2024-02-20]. .
[57]	张敏.东南景天镉超积累相关基因的克隆与功能研究[D].杭州:浙江大学,2011.
	ZHANG M. Cloning and functional analysis of selected cd hyperaccumulation related genes from sedum alfredit hance [D]. Hangzhou: Zhejiang University, 2011.