关键词: 玉米, SCMV, 基因组, 多样性

Abstract: Maize is an important food and economic crop, which plays an important role in the national economy. Sugercan mosaic virus widely distributed in the main maize producing areas in the world, and it is a great threat for the maize production. For controling SCMV, it is necessary to systematically study the damage, genetic structure and evolution mechanism. This study collected 122 maize leaf samples (62 samples in 2015 and 60 samples in 2016) from four maize producing areas in Shanxi province. By RT-PCR, 36 samples (20 in 2015 and 16 in 2016) were proved to be positive for SCMV. One new SCMV strain was isolated and sequenced from these positive samples. It was found that the genome of this isolate was 9 539 bp including 5′-UTR and 3′-UTR  region and encoded 3 063 aa. The identity and phylogenetic analysis with  26 isolates reported online NCBI were carried out, it was found the 27 isolates were divided into 3 different evolutionary populations based on the whole-genome. The results of this study provided a basis for assessing the epidemiological characteristics of SCMV in China and contribute to the formulation of long-term sustainable prevention and control strategies for SCMV.

Key words: maize, SCMV, genome, diversity