








中国农业科技导报 ›› 2022, Vol. 24 ›› Issue (2): 193-200.DOI: 10.13304/j.nykjdb.2020.1085
马兴东1(
), 郭晔红1(
), 李梅英2, 于霞霞3, 徐英杰1, 朱文娟1, 冯洁1
收稿日期:2020-12-19
接受日期:2021-03-04
出版日期:2022-02-15
发布日期:2022-02-22
通讯作者:
郭晔红
作者简介:马兴东 E-mail: maxingdong183@163.com;
基金资助:
Xingdong MA1(
), Yehong GUO1(
), Meiying LI2, Xiaxia YU3, Yingjie XU1, Wenjuan ZHU1, Jie FENG1
Received:2020-12-19
Accepted:2021-03-04
Online:2022-02-15
Published:2022-02-22
Contact:
Yehong GUO
摘要:
为明确干旱区栽培黑果枸杞(Lyciumruthenicum Murr.)抗旱生理对施氮(N)的响应,以尿素(含氮量46%)为氮源,于2018—2019年,设置0(CK)、50(N1)、100(N2)、150(N3)和200 g·株-1(N4)共5个施氮量梯度,在干旱胁迫0(土壤含水量30.8%和29.3%)、15(土壤含水量21.7%和21.1%)和30 d(土壤含水量11.2%和9.7%),对黑果枸杞叶片中超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性和丙二醛(MDA)、脯氨酸(Pro)、可溶性糖(SS)含量进行测定,并通过隶属函数法对黑果枸杞抗旱性进行综合评价。结果表明,适量施氮可以提高黑果枸杞叶片中SOD、POD、CAT活性和Pro、SS含量,降低MDA含量;当施氮量为150~200 g·株-1时,叶片中SOD、POD和CAT活性与SS含量较高;当施氮量为100~150 g·株-1时,叶片Pro含量较高,MDA含量较低。对黑果枸杞抗旱性的综合评价表明,适量施氮有利于增强黑果枸杞的抗旱性,在干旱胁迫0和15 d时, 黑果枸杞在100~150 g·株-1施氮量时抗旱能力最强;在干旱胁迫30 d,施氮量为100 g·株-1时黑果枸杞的抗旱能力最强,为干旱区黑果枸杞的栽培模式提供了理论依据和技术支撑。
中图分类号:
马兴东, 郭晔红, 李梅英, 于霞霞, 徐英杰, 朱文娟, 冯洁. 不同施氮量下黑果枸杞对干旱胁迫的响应[J]. 中国农业科技导报, 2022, 24(2): 193-200.
Xingdong MA, Yehong GUO, Meiying LI, Xiaxia YU, Yingjie XU, Wenjuan ZHU, Jie FENG. Response of Drought Stress of Lyciumruthenicum Murr. Under Different Nitrogen Applications[J]. Journal of Agricultural Science and Technology, 2022, 24(2): 193-200.
图1 不同施氮量下黑果枸杞叶片中SOD、POD和CAT活性注:不同小写字母表示相同干旱胁迫不同施氮量处理间差异在P<0.05水平具有显著性。
Fig.1 Activities of SOD, POD and CAT in leaf of Lyciumruthenicum Murr under different N applicationsNote:Different lowercase letters indicate significant difference between different N applications at same drought stress at P<0.05 level.
图2 不同施氮量下黑果枸杞叶片MDA含量注:不同小写字母表示相同干旱胁迫不同施氮量处理间差异在P<0.05水平具有显著性。
Fig.2 MDA contents in leaf of Lyciumruthenicum Murr under different N applicationsNote:Different lowercase letters indicate significant difference between different N applications at same drought stress at P<0.05 level.
图3 不同施氮量下黑果枸杞叶片Pro和SS含量注:不同小写字母表示相同干旱胁迫不同施氮量处理间差异在P<0.05水平具有显著性。
Fig.3 Content of Pro and SS in leaf of Lyciumruthenicum Murr under different N applicationsNote:Different lowercase letters indicate significant difference between different N applications at same drought stress at P<0.05 level.
| 干旱胁迫天数 Drought stress days/d | 年份 Year | 氮处理 N treatment | SOD | POD | CAT | MDA | Pro | SS | 综合评价值Comprehensive evaluation value | 抗旱性排序Rank of drought-resistant |
|---|---|---|---|---|---|---|---|---|---|---|
| 0 | CK | 0.455 | 0.429 | 0.350 | 0.366 | 0.165 | 0.255 | 0.337 | 3 | |
| N1 | 0.367 | 0.588 | 0.467 | 0.411 | 0.375 | 0.379 | 0.431 | 2 | ||
| 2018 | N2 | 0.422 | 0.323 | 0.385 | 0.706 | 0.417 | 0.412 | 0.444 | 1 | |
| N3 | 0.277 | 0.180 | 0.261 | 0.661 | 0.292 | 0.339 | 0.335 | 4 | ||
| N4 | 0.194 | 0.367 | 0.159 | 0.444 | 0.138 | 0.240 | 0.257 | 5 | ||
| CK | 0.217 | 0.183 | 0.159 | 0.593 | 0.305 | 0.497 | 0.326 | 5 | ||
| N1 | 0.342 | 0.586 | 0.112 | 0.398 | 0.258 | 0.494 | 0.365 | 3 | ||
| 2019 | N2 | 0.442 | 0.295 | 0.313 | 0.599 | 0.301 | 0.614 | 0.427 | 2 | |
| N3 | 0.520 | 0.361 | 0.278 | 0.613 | 0.417 | 0.511 | 0.450 | 1 | ||
| N4 | 0.213 | 0.192 | 0.396 | 0.443 | 0.287 | 0.483 | 0.336 | 4 | ||
| 15 | CK | 0.547 | 0.516 | 0.421 | 0.440 | 0.198 | 0.307 | 0.405 | 5 | |
| N1 | 0.441 | 0.707 | 0.561 | 0.494 | 0.451 | 0.456 | 0.518 | 2 | ||
| 2018 | N2 | 0.507 | 0.388 | 0.463 | 0.849 | 0.501 | 0.495 | 0.534 | 1 | |
| N3 | 0.333 | 0.516 | 0.314 | 0.795 | 0.351 | 0.708 | 0.503 | 3 | ||
| N4 | 0.233 | 0.441 | 0.491 | 0.534 | 0.666 | 0.289 | 0.442 | 4 | ||
| CK | 0.561 | 0.220 | 0.191 | 0.713 | 0.367 | 0.598 | 0.442 | 4 | ||
| N1 | 0.411 | 0.705 | 0.135 | 0.478 | 0.310 | 0.594 | 0.439 | 5 | ||
| 2019 | N2 | 0.531 | 0.355 | 0.376 | 0.720 | 0.362 | 0.738 | 0.514 | 2 | |
| N3 | 0.625 | 0.434 | 0.334 | 0.737 | 0.501 | 0.614 | 0.541 | 1 | ||
| N4 | 0.256 | 0.531 | 0.476 | 0.533 | 0.345 | 0.581 | 0.454 | 3 | ||
| 30 | CK | 0.552 | 0.409 | 0.579 | 0.605 | 0.273 | 0.422 | 0.473 | 4 | |
| N1 | 0.607 | 0.472 | 0.572 | 0.390 | 0.320 | 0.527 | 0.481 | 3 | ||
| 2018 | N2 | 0.698 | 0.534 | 0.637 | 0.167 | 0.690 | 0.681 | 0.568 | 1 | |
| N3 | 0.458 | 0.710 | 0.432 | 0.493 | 0.483 | 0.373 | 0.492 | 2 | ||
| N4 | 0.321 | 0.607 | 0.676 | 0.334 | 0.416 | 0.397 | 0.458 | 5 | ||
| CK | 0.571 | 0.303 | 0.263 | 0.681 | 0.504 | 0.522 | 0.474 | 4 | ||
| N1 | 0.566 | 0.669 | 0.185 | 0.658 | 0.427 | 0.517 | 0.504 | 3 | ||
| 2019 | N2 | 0.531 | 0.488 | 0.518 | 0.691 | 0.498 | 0.415 | 0.523 | 1 | |
| N3 | 0.560 | 0.597 | 0.460 | 0.314 | 0.590 | 0.545 | 0.511 | 2 | ||
| N4 | 0.352 | 0.230 | 0.155 | 0.433 | 0.475 | 0.299 | 0.324 | 5 |
表1 不同施氮量下黑果枸杞综合生理指标的平均隶属函数值
Table 1 Average subordinate function values of different N application rates for Lyciumruthenicum Murr.
| 干旱胁迫天数 Drought stress days/d | 年份 Year | 氮处理 N treatment | SOD | POD | CAT | MDA | Pro | SS | 综合评价值Comprehensive evaluation value | 抗旱性排序Rank of drought-resistant |
|---|---|---|---|---|---|---|---|---|---|---|
| 0 | CK | 0.455 | 0.429 | 0.350 | 0.366 | 0.165 | 0.255 | 0.337 | 3 | |
| N1 | 0.367 | 0.588 | 0.467 | 0.411 | 0.375 | 0.379 | 0.431 | 2 | ||
| 2018 | N2 | 0.422 | 0.323 | 0.385 | 0.706 | 0.417 | 0.412 | 0.444 | 1 | |
| N3 | 0.277 | 0.180 | 0.261 | 0.661 | 0.292 | 0.339 | 0.335 | 4 | ||
| N4 | 0.194 | 0.367 | 0.159 | 0.444 | 0.138 | 0.240 | 0.257 | 5 | ||
| CK | 0.217 | 0.183 | 0.159 | 0.593 | 0.305 | 0.497 | 0.326 | 5 | ||
| N1 | 0.342 | 0.586 | 0.112 | 0.398 | 0.258 | 0.494 | 0.365 | 3 | ||
| 2019 | N2 | 0.442 | 0.295 | 0.313 | 0.599 | 0.301 | 0.614 | 0.427 | 2 | |
| N3 | 0.520 | 0.361 | 0.278 | 0.613 | 0.417 | 0.511 | 0.450 | 1 | ||
| N4 | 0.213 | 0.192 | 0.396 | 0.443 | 0.287 | 0.483 | 0.336 | 4 | ||
| 15 | CK | 0.547 | 0.516 | 0.421 | 0.440 | 0.198 | 0.307 | 0.405 | 5 | |
| N1 | 0.441 | 0.707 | 0.561 | 0.494 | 0.451 | 0.456 | 0.518 | 2 | ||
| 2018 | N2 | 0.507 | 0.388 | 0.463 | 0.849 | 0.501 | 0.495 | 0.534 | 1 | |
| N3 | 0.333 | 0.516 | 0.314 | 0.795 | 0.351 | 0.708 | 0.503 | 3 | ||
| N4 | 0.233 | 0.441 | 0.491 | 0.534 | 0.666 | 0.289 | 0.442 | 4 | ||
| CK | 0.561 | 0.220 | 0.191 | 0.713 | 0.367 | 0.598 | 0.442 | 4 | ||
| N1 | 0.411 | 0.705 | 0.135 | 0.478 | 0.310 | 0.594 | 0.439 | 5 | ||
| 2019 | N2 | 0.531 | 0.355 | 0.376 | 0.720 | 0.362 | 0.738 | 0.514 | 2 | |
| N3 | 0.625 | 0.434 | 0.334 | 0.737 | 0.501 | 0.614 | 0.541 | 1 | ||
| N4 | 0.256 | 0.531 | 0.476 | 0.533 | 0.345 | 0.581 | 0.454 | 3 | ||
| 30 | CK | 0.552 | 0.409 | 0.579 | 0.605 | 0.273 | 0.422 | 0.473 | 4 | |
| N1 | 0.607 | 0.472 | 0.572 | 0.390 | 0.320 | 0.527 | 0.481 | 3 | ||
| 2018 | N2 | 0.698 | 0.534 | 0.637 | 0.167 | 0.690 | 0.681 | 0.568 | 1 | |
| N3 | 0.458 | 0.710 | 0.432 | 0.493 | 0.483 | 0.373 | 0.492 | 2 | ||
| N4 | 0.321 | 0.607 | 0.676 | 0.334 | 0.416 | 0.397 | 0.458 | 5 | ||
| CK | 0.571 | 0.303 | 0.263 | 0.681 | 0.504 | 0.522 | 0.474 | 4 | ||
| N1 | 0.566 | 0.669 | 0.185 | 0.658 | 0.427 | 0.517 | 0.504 | 3 | ||
| 2019 | N2 | 0.531 | 0.488 | 0.518 | 0.691 | 0.498 | 0.415 | 0.523 | 1 | |
| N3 | 0.560 | 0.597 | 0.460 | 0.314 | 0.590 | 0.545 | 0.511 | 2 | ||
| N4 | 0.352 | 0.230 | 0.155 | 0.433 | 0.475 | 0.299 | 0.324 | 5 |
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