关键词: 二马牙, 长脖, 表型, 人参皂苷, 转录组

Abstract: To reveal the phenotype and ginsenoside content differences of the Changbo and the Ermaya, this paper measured the phenotype and ginsenoside content of two kinds of 14-year-old forest cultivated  Panax ginseng, and high-throughput transcriptome sequencing was used to analyze the molecular mechanism of the differences between the leaves, the rhizoma and the root of the two types of ginseng to screen the differentially expressed genes, and then 7 important genes were verificatified in the rhizoma using qRT-PCR. The rhizoma of  Ermaya  was more shorter and thicker than that of Changbo, and it had high yield. A total of 124 differentially expressed genes were obtained by transcriptome analysis, of which 76 were down-regulated and 48 were up-regulated in leaves. A total of 604 differentially expressed genes were obtained by transcriptome analysis, of which 321 were down-regulated and 283 were up-regulated in rhizoma. A total of 89 differentially expressed genes were obtained by transcriptome analysis, of which 58 were down-regulated and 31 were up-regulated in leaves.Through enrichment analysis of KEGG metabolic pathway,differentially expressed genes were enriched into 16, 70 and 17 metabolic pathways. KEGG enrichment analysis demonstrated differential genes were mainly annotated to biological functions such as starch and sucrose metabolism, plant hormone signal transduction, phenylpropanoid biosynthesis. The results provided theoretical references for the selection of species and the molecular mechanism that reveals the differences of forest cultivated Panax ginseng.

Key words: Ermaya, Changbo, phenotype, ginsenoside, transcriptome