关键词: 高通量测序, 蚕豆, 内生细菌, 多样性

Abstract: In order to analyze the species abundance and diversity of endophytic bacteria in  faba bean seeds,taking Japans white skin (S18P23.1, S18P23.2) and Qidou 2 (S18P24.1, S18P24.2) as materials, 133  855 effective sequences were obtained  by Illumina MiSeq high-throughput sequencing of the 16S rRNA V3～V4 region. The sequence with similarity above 97% was divided into one operational taxonomy unit (OTU), so 1 598 OTUs were obtained after optimization. The results showed that majority of the endophytic bacteria belonged to Bacteroidetes (30%～33%），Proteobacteria（23%～25%）, Firmicutes（23%～25%）, and Actinobacteria （5%～7%）, which were therefore the dominant bacterial Phyla in these four faba bean seeds． The dominant genera at genus level in these endophytic bacteria sequenced from faba bean seeds were different. Above results indicated faba bean seeds were rich in endophytic bacteria, the abundance of probiotics in one faba bean variety (Qidou 2) were higher than another variety (Japans white skin).

Key words: high-throughput sequencing, faba bean seed, endophytic bacterium, diversity