关键词: 映山红, 定量, 花色苷组分, 花色描述

Abstract: To further understand of the flower coloration mechanism of Rhododendron simsii, the anthocyanin compositions of seven lines were investigated, which provided the scientific basis for the flower color improvement for R. simsii. The Royal Horticultural Society Color Chart and CIE  system were used to describe the chromatic features of the R. simsii lines. The anthocyanin compositions of petals extracts were analyzed and quantified by the UPLC-Q-TOF-MS system combining with standard curve method.  The results showed that the flower colors of seven lines of R. simsii were categorized into three groups: red group, red-purple group and purple group. Eleven anthocyanins in petals were identified according to the standard substances and references as cyanidin 3-O-arabinoside, cyanidin 3-O-glucoside, cyanidin 3-O-galactoside, delphindin 3-O-arabinoside, delphindin 3-O-glucoside, malvidin 3-O-arabinoside, malvidin 3-O-glucoside, peonindin 3-O-arabinoside, peonindin 3-O-galactoside, petunidin 3-O-arabinoside and petunidin 3-O-glucoside. The red-flowered lines mainly contained cyanidin monoglycosides and peonindin monoglycosides, while the purple-flowered lines contained delphindin monoglycosides and malvidin monoglycosides. The results also showed that the anthocyanins content in red-flowered was much higher than that in red-purple and purple flowers. These results suggested the involvement of transcription factors or key genes in the up-regulation of the anthocyanin biosynthetic pathway in the red-flowered lines.

Key words: Rhododendron simsii, quantification, anthocyanin, floral pigmentation