关键词: 同位素标记相对和绝对定量技术（iTRAQ）, 水稻, 蛋白质组学, 胁迫, 激素, 突 变体

Abstract: Isobaric tags for relative and absolute quantitation (iTRAQ) is a novel high-throughput technique for protein sequencing. This technology combined with high-resolution mass spectrometer is a powerful means for carrying out qualitative and quantitative analysis up to 8 samples simultaneously. This method can test wide range of protein with low limitation, and yet has reliabal and accurate results. Recently, iTRAQ technology has been widely used in studying plant structure, function and expression, etc.. Rice (Oryza sativa L.) is a main grain crop for human, and also the first cereal model plant completed genome sequencing. It is of great significance to study rice growth, development and regulation mechanism at the proteome level. This paper summarized the principle, operation procedure, advantages and disadvantages of iTRAQ technology, and focused on analyzing rice proteomics research in plant tissue and organ, subcellular level, environment stress, hormone induce, and rice mutant, etc.. Finally this paper prospected the application of iTRAQ technology in rice proteomics.

Key words: isobaric tags for relative and absolute quantitation (iTRAQ), rice, proteomics, stress, hormone, mutant