关键词: 游牧, 定居, 高通量测序, 真菌群落, 土壤理化性质

Abstract: Taking the S. transiliense desert degraded grassland in the northern slope of Tianshan Mountain as the research object, this paper studied the effects of nomadic and settled on soil properties, soil fungi community composition and diversity in S. transiliense desert grassland by setting two gradients of light grazing (LG) and heavy grazing (HG) in nomadic and settled plots. The results showed that ：The content of soil organic carbon and available phosphorus in nomadic plots were significantly higher than that in settled plots (P<0.05), and the content of LG-N was the highest. A total of 590 662 valid sequences and 7 124 OTUs were detected in soil samples, including 8 phyla, 337 genera of fungi in which Ascomycetes and Basidiomycetes were dominant phylum, and  Mycocentrospora and Penicillium were the dominant genus of nomadic and settled plots respectively. The relative abundance of unidentified genera reached 41.73% and 54.31% respectively. The Chao1 richness index of the LG-N was significantly higher than that of the LG-S, which were 730.350 and 497.446, respectively. There was no significant difference in the Shannon diversity index. Correlation analysis showed that soil organic carbon and available phosphorus were significantly positively correlated with Ascomycota and Zygomycetes, and negatively correlated with Unidentified; soil total phosphorus, available phosphorus and Glomeromycota, soil total nitrogen, available nitrogen and Rozellomycota gates showed significantly positive correlation. In summary, the nomadic light grazing soil fungi information was abundant, which could provide theoretical basis for the restoration and sustainable development of the degraded grassland of S. transiliense desert degraded grassland.

Key words: nomadic grazing, settled grazing, high-throughput sequencing, fungi community, soil physical and chemical properties