关键词: 氟节胺, 烤烟, 碳氮代谢, 酶活性, 叶片超微结构

Abstract: This paper studied the carbon and nitrogen metabolism, chemical components and ultrastructures of flue-cured tobacco under effects of three treatments, i. e. artificial excepting the bud (T1), pouring flumetralin 1 time by cup(T2), pouring flumetralin 2 times by cup(T3). The results showed：①The activities of amylase, invertase and sucrose synthase of T2 were significantly higher than T1 at 21 d and 28 d after topping. ②At 14 d, 21 d and 28 d after topping, the activity of NR of T2 was significantly higher than T1 and T3; the activity of GS of T2 was significantly higher than T3; T2 and T1 had no significant difference in GDH activity, but both were significantly higher than T3. ③The contents of total sugar and reducing sugar were T2>T1>T3; the content of starch was T2>T3>T1; at 14 d, 21 d and 28 d after topping, the content of total nitrogen of T2 was significantly higher than T1; the contents of nicotine of T2 and T3 were significantly lower than T1. ④The leaf of artificial inhibition bud developed normally, and the ultrastructure of the leaves of pouring prime was destroyed to some extent. The carbon and nitrogen metabolism was enhanced and coordinated, and leaf development was more reasonable for pouring prime 1 time by cup.

Key words: flumetralin, flue-cured tobacco, carbon and nitrogen metabolism, enzyme activity, leaf ultrastructure