关键词: 乙草胺, 降解菌, 枯草芽孢杆菌, 盆栽效果, 土壤修复

Abstract: The acetochlor-contaminated soil could be repaired by microbial technology, and a high-efficient acetochlor-degrading bacterium named as Bacillus subtilus L3 has been obtained after a series of isolation, selection and identification. In order to further verifiy its actual degradation effects on acetochlor and its restoration effects on contaminated soil, the pot experiment was conducted in this paper. Firstly, this test inspected the effects of degraded bacteria concentration and the degraded time on the degradation of acetochlor in the soil, and secondly, of the application of strain L3 on the amount of indigenous microorganisms, enzyme activity, and nutrient elements in the soil, and on the growth of sensitive crop wheat. The results showed that the final concentration of bacterial liquid which had the highest degradation rate was 5×108 CFU/g soil, and its degradation rate was 92.65% after 50 d; the application of L3 bacteria liquid could recover the edaphon amount to a great degree; invertase activity, urease activity, protease activity and catalase activity increased respectively by 100%, 260%, 56% and 6%; total nitrogen content, rapid available phosphorus content, effective potassium content and the content of organic matter in soil increased respectively by 77%, 159%, 698% and 274%; compared with the control, wheat aerial part heightened 117%, the total fresh weight gained 56%, and root length grew by 27%. The results confirmed a good acetochlor-degrading effect and soil restoration capacity of strain L3, and laid a theoretical foundation for the practical application of strain L3 in soil pollution regulation and restoration.

Key words: acetochlor, degrading bacterium, Bacillus subtilis, potted effect, soil restoration