关键词: 头孢菌素, 降解菌, 筛选, 功能鉴定

Abstract: In order to conduct rational and pollution-free utilization of waste cephalosporin drug residues, this paper took the soil loaded for a long time by cephalosporin drug residues as specimen source; obtained leaching liquid from the soil; and screened out the strains with ability of cephalosporin antibiotic degradation by the 3rd generation of cephalosporin drug cefixime. Morphological observation was carried on this strain by gram staining and double staining electron microscope ultrathin section technique, and gene sequence analysis was conducted on 16S rDNA. The growth characteristics and optimum degradation conditions of the strain were studied by single factor control variable method. The results showed that: This strain was a bacillus without spore; it was confirmed as genus Achromobacter and named as Achromobacter sp.YF-1 through 16S rDNA sequence alignment; under the conditions of temperature 37℃, pH 6~7, revolving speed 120 r/min for 7 d cultivation, the degradation rate of cephalosprins could reach 92.71%; the degradation ability to cephalosprins of this strain was stable, and could be used to treat cephalosporin antibiotics residual.

Key words: cephalosporin, degrading bacteria, screening, function identification