关键词:
农杆菌, 转基因棉花, 耐旱耐盐, 生理指标, 相对表达量

Abstract: Transcription factor gene PeDREB2a and KcERF were inserted to the upland cotton R15 mediated by agrobacterium and 12 strains of transgenic plants were obtained. Through kanamycin screening and molecular testing, 7 generation strains with PeDREB2a and KcERF were obtained. This paper measured the physiological indexes related to stress tolerance and analyzed relative exprsssion of genes, and the results showed that under the stress of 200 mmol/L NaCl and 15% PEG-6000, the activity of CAT and SOD, free proline content of transgenic cotton seedling  were higher than the control, the MDA content significantly lower than control. Real-time PCR results showed that under drought stress, PeDREB2a relative expression in transgenic cotton seedling leaf was higher than KcERF; under salt stress, PeDREB2a and KcERF relative expression in transgenic cotton was flat. This suggested that KcERF-PeDREB2a contributed to improve the drought and salt tolerance ability in cotton.

Key words: Agrobacterium, transgenic cotton, drought and salt stress, physiological indexes, relative expression