关键词: 水稻种子, 抗病性, 固有细菌群落, 多样性, 16S rRNA基因克隆文库

Abstract: The indigenous bacterial community structures of seeds harvested in 2 consecutive years from 2 northern rice varieties ‘Yuefu’ and ‘Jindao 305’ with different disease-resistance were studied via establishing bacteria 16S rRNA gene clone library. In 2008, 211 and 174 positive clones, 22 and 25 OTUs were gained from library of ‘Yuefu’ and ‘Jindao 305’, and in 2009, 164 and 137 positive clones, 11 and 19 OTUs were obtained. Four bacteria groups were detected in these 2 varieties: α-Proteobacteria, β-Proteobacteria, γ-Proteobacteria and Actinobacteria. Among them α-Proteobacteria and γ-Proteobacteria were dominant groups. It was discovered that more abundant genera, and more stable community structure of seed indigenous bacteria was harbored in ‘Jindao 305’, which had stronger disease-resistance than ‘Yuefu’.

Key words: rice seed, disease resistance, indigenous bacterial community, diversity, 16S rRNA gene clone library