Abstract:

In order to expound the functions of cloned C-terminal binding protein gene OsCtBP-A, we established its overexpression and RNAi constructs and transformed them into calli of rice (cv. Nipponbare) via Agrobacterium-mediated transformation. Forty-five transgenic plants with overexpression and 75 with RNAi were generated and identified by PCR and GUS staining analysis. In T1 progeny of transgenic rice lines with overexpression, OsCtBP-A gene expression was remarkably increased, and root development was enhanced during seedling stage, while its resistance to rice leaf blight was no much changed. In contrast, OsCtBP-A gene expression was significantly reduced, and the root development at seedling stage was slowed down and its resistance to drought was weakened in RNAi silenced lines. The results suggested that OsCtBP-A gene might be involved in regulation of root development at seedling stage and resistances to drought and diseases.

Key words: OsCtBP-A, overexpression, RNAi-mediated silence, root development, stress resistance

摘要：

为了阐明己克隆的水稻C端结合蛋白家族基因OsCtBP-A的功能,构建了该基因的过量表达和RNAi沉默载体,通过农杆菌介导转化水稻品种日本晴愈伤组织,分别获得了45个过量表达和75个RNAi 沉默T0代转基因植株,并进行了PCR和GUS染色鉴定。T1代过量表达株OsCtBP-A基因表达量明显增加,苗期根系生长增加,对水稻白叶枯病抗性无明显变化；而基因沉默株OsCtBP-A基因表达量显著下降,苗期根系发育迟缓,抗旱性明显降低,抗病性减弱。表明OsCtBP-A基因的表达可能对水稻苗期根系发育、抗旱性和抗病性具有不同程度的调控作用。

关键词: OsCtBP-A;过量表达;RNAi沉默;根系发育;抗性