Genome-wide Identification and Expression Analysis of Maize JRL Gene Family

doi:10.13304/j.nykjdb.2024.0310

Abstract

Abstract:

Jacalin-related lectin （JRL） proteins belong to the plant lectin family， exhibit specific sugar-binding activity and play crucial roles in processes such as plant stress response. To understand the function of the maize JRL （ZmJRL） gene family， the bioinformatics methods were used to identify and analyze the ZmJRL genes. Additionally， the transcriptomic data from the MaizeGDB website were utilized to investigate the specific expression of ZmJRL genes in various tissues and under different stress conditions. The results revealed that a total of 16 ZmJRL genes in maize were identified， designated as ZmJRL01 to ZmJRL16. They were unevenly distributed on 7 chromosomes of maize. These proteins exhibited structural similarity， possessing jacalin domains and mostly being alkaline-stable proteins. Subcellular localization analysis indicated that ZmJRL proteins primarily localized in the cytoplasm， nucleus and chloroplasts. Phylogenetic analysis divided maize JRL proteins into 3 clades， which were closely related to rice （Oryza sativa L.）. The ZmJRL proteins with same clades had similar motifs. The promoter of ZmJRL genes contained various cis-elements that respond to abiotic stress and hormones. Transcriptome analysis showed that the ZmJRL genes had tissue expression specificity， and the majority of ZmJRL genes could respond to at least one type of adversity stress， such as cold， heat， salt and drought. Above results provided a reference for further study on the biological functions of ZmJRL genes.

Key words: maize, JRL protein, ZmJRL gene family, bioinformatics

摘要：

JRL（jacalin-related lectin）蛋白是植物凝集素家族成员之一，具有特异糖结合活性，在植物胁迫响应等过程中发挥重要作用。为解析玉米JRL（ZmJRL）基因家族功能，利用生物信息学方法对ZmJRL基因进行鉴定与分析，并利用MaizeGDB网站公布的转录组数据检测ZmJRL基因在不同组织和不同胁迫处理下的差异表达情况。结果显示，玉米中共有16个ZmJRL基因，命名为ZmJRL01~ZmJRL16，不均匀分布在玉米7条染色体上，它们的蛋白结构相似，均具有jacalin结构域，多数为碱性稳定蛋白。亚细胞定位结果显示，ZmJRL蛋白主要定位于细胞质、细胞核、叶绿体等部位。系统进化分析将玉米JRL蛋白分为3个类群，与水稻亲缘关系较近，相同类群的ZmJRL蛋白具有相似的保守基序。ZmJRL基因启动子区具有多种非生物胁迫和激素胁迫响应元件。转录组数据分析表明，ZmJRL基因表达具有组织特异性，多数ZmJRL基因能对冷、热、盐、干旱等至少1种逆境胁迫响应表达。以上研究结果为进一步探究ZmJRL基因的生物学功能提供了基础。

关键词: 玉米, JRL蛋白, ZmJRL基因家族, 生物信息学

CLC Number:

S513

Pan ZHANG, Haichao SUN, Wenheng DONG, Yongjiang LI, Yingying ZHANG, Lili SHI, Jun ZHAO, Daowen LU. Genome-wide Identification and Expression Analysis of Maize JRL Gene Family[J]. Journal of Agricultural Science and Technology, 2025, 27(10): 32-41.

张盼, 孙海潮, 董文恒, 李永江, 张莹莹, 史丽丽, 赵鋆, 卢道文. 玉米JRL基因家族全基因组鉴定及表达分析[J]. 中国农业科技导报, 2025, 27(10): 32-41.

Figures/Tables 8

References 27

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基因名称 Gene name	基因编号 Gene ID	氨基酸数 No. of amino acid/aa	分子量 Molecular Weight/Da	等电点 pI	稳定系数 Stability coefficient	脂肪酸指数 Aliphatic index	疏水性 Hydrophob-icity	亚细胞定位 Subcellular location
ZmJRL01	Zm00001eb097060_T001	169	18 251.73	5.90	18.73	85.33	0.037	细胞质Cytoplasm
ZmJRL02	Zm00001eb097070_T001	315	32 911.95	5.16	20.74	80.13	-0.061	细胞质Cytoplasm
ZmJRL03	Zm00001eb167140_T001	783	85 242.19	7.22	33.37	76.55	-0.319	细胞质Cytoplasm
ZmJRL04	Zm00001eb195670_T001	309	33 061.08	9.69	43.86	70.10	-0.294	线粒体Mitochondria
ZmJRL05	Zm00001eb265850_T001	326	34 366.71	7.73	40.32	77.76	-0.058	叶绿体Chloroplast
ZmJRL06	Zm00001eb265880_T001	322	34 528.51	7.75	39.96	72.05	-0.255	叶绿体Chloroplast
ZmJRL07	Zm00001eb265900_T002	418	44 481.42	9.14	36.21	80.74	-0.094	细胞质膜Plasmalemma
ZmJRL08	Zm00001eb265890_T001	323	34 268.25	6.86	42.61	77.28	-0.207	细胞核Nucleus
ZmJRL09	Zm00001eb292320_T002	596	64 567.13	8.20	39.58	68.12	-0.335	细胞核Nucleus
ZmJRL10	Zm00001eb304540_T001	306	32 064.18	5.96	23.30	87.29	0.106	细胞质Cytoplasm
ZmJRL11	Zm00001eb342390_T001	204	22 185.15	9.16	46.33	71.18	-0.359	细胞质Cytoplasm
ZmJRL12	Zm00001eb348500_T001	477	52 460.13	5.49	35.58	77.69	-0.375	细胞核Nucleus
ZmJRL13	Zm00001eb355410_T001	281	29 855.36	6.17	31.04	74.95	-0.243	细胞质Cytoplasm
ZmJRL14	Zm00001eb369990_T001	357	39 150.11	9.02	31.46	66.81	-0.304	细胞质Cytoplasm
ZmJRL15	Zm00001eb392870_T001	199	21 470.85	5.26	29.66	66.13	-0.534	细胞骨架Cytoskeleton
ZmJRL16	Zm00001eb407630_T001	151	16 520.55	6.72	29.88	68.48	-0.454	细胞质Cytoplasm

基因名称 Gene name	基因编号 Gene ID	氨基酸数 No. of amino acid/aa	分子量 Molecular Weight/Da	等电点 pI	稳定系数 Stability coefficient	脂肪酸指数 Aliphatic index	疏水性 Hydrophob-icity	亚细胞定位 Subcellular location
ZmJRL01	Zm00001eb097060_T001	169	18 251.73	5.90	18.73	85.33	0.037	细胞质Cytoplasm
ZmJRL02	Zm00001eb097070_T001	315	32 911.95	5.16	20.74	80.13	-0.061	细胞质Cytoplasm
ZmJRL03	Zm00001eb167140_T001	783	85 242.19	7.22	33.37	76.55	-0.319	细胞质Cytoplasm
ZmJRL04	Zm00001eb195670_T001	309	33 061.08	9.69	43.86	70.10	-0.294	线粒体Mitochondria
ZmJRL05	Zm00001eb265850_T001	326	34 366.71	7.73	40.32	77.76	-0.058	叶绿体Chloroplast
ZmJRL06	Zm00001eb265880_T001	322	34 528.51	7.75	39.96	72.05	-0.255	叶绿体Chloroplast
ZmJRL07	Zm00001eb265900_T002	418	44 481.42	9.14	36.21	80.74	-0.094	细胞质膜Plasmalemma
ZmJRL08	Zm00001eb265890_T001	323	34 268.25	6.86	42.61	77.28	-0.207	细胞核Nucleus
ZmJRL09	Zm00001eb292320_T002	596	64 567.13	8.20	39.58	68.12	-0.335	细胞核Nucleus
ZmJRL10	Zm00001eb304540_T001	306	32 064.18	5.96	23.30	87.29	0.106	细胞质Cytoplasm
ZmJRL11	Zm00001eb342390_T001	204	22 185.15	9.16	46.33	71.18	-0.359	细胞质Cytoplasm
ZmJRL12	Zm00001eb348500_T001	477	52 460.13	5.49	35.58	77.69	-0.375	细胞核Nucleus
ZmJRL13	Zm00001eb355410_T001	281	29 855.36	6.17	31.04	74.95	-0.243	细胞质Cytoplasm
ZmJRL14	Zm00001eb369990_T001	357	39 150.11	9.02	31.46	66.81	-0.304	细胞质Cytoplasm
ZmJRL15	Zm00001eb392870_T001	199	21 470.85	5.26	29.66	66.13	-0.534	细胞骨架Cytoskeleton
ZmJRL16	Zm00001eb407630_T001	151	16 520.55	6.72	29.88	68.48	-0.454	细胞质Cytoplasm

响应类型 Response type	顺式元件 Cis-element	基因数量 Gene number
脱落酸Abscisic acid	ABRE	14
生长素Auxin	AuxRR-core	4
	circadian	1
	TGA-element	6
赤霉素Gibberellin	GARE-motif	4
赤霉素Gibberellin	P-box	5
茉莉酸甲酯Methyl jasmonate	CGTCA-motif	15
茉莉酸甲酯Methyl jasmonate	TGACG-motif	15
水杨酸Salicylic acid	TCA-element	4
厌氧Anaerobic induction	ARE	12
防卫胁迫Defense and stress	TC-rich repeats	5
低温Low-temperature	LTR	7
机械损伤Wound	WUN-motif	6
干旱Drought	MBS	6

响应类型 Response type	顺式元件 Cis-element	基因数量 Gene number
脱落酸Abscisic acid	ABRE	14
生长素Auxin	AuxRR-core	4
	circadian	1
	TGA-element	6
赤霉素Gibberellin	GARE-motif	4
赤霉素Gibberellin	P-box	5
茉莉酸甲酯Methyl jasmonate	CGTCA-motif	15
茉莉酸甲酯Methyl jasmonate	TGACG-motif	15
水杨酸Salicylic acid	TCA-element	4
厌氧Anaerobic induction	ARE	12
防卫胁迫Defense and stress	TC-rich repeats	5
低温Low-temperature	LTR	7
机械损伤Wound	WUN-motif	6
干旱Drought	MBS	6