Dynamic Changes of Aluminium Ion in the Sepals and Leaves of Hydrangea macrophylla ‘Bailmer’

doi:10.13304/j.nykjdb.2021.1110

Abstract

Abstract:

Hydrangea macrophylla is an aluminum（Al）-accumulating plant with great ornamental characters. Al ion plays an important role in the sepal bluing， in flower color regulation， as well as in the plant growth and development. Taking ‘Bailmer’ as material，this paper determined the optimum growth temperature and studied the dynamic changes of Al ion in sepals and leaves. The results showed that 23 °C was beneficial for plant growth and uptaking of Al ion in leaves and sepals. The dynamic change trends of Al ion content in the sepals and leaves were similar， which first increased， then decreased， and finally increased. Uptaking rates of Al ion in the sepals and leaves increased rapidly at the early stage， reached to the peak at 12 h， and then decreased rapidly to be stable. The results laid a theoretical foundation for flower color regulation of H. macrophylla.

Key words: Hydrangea macrophylla ‘Bailmer’, temperature, Aluminium ion absorption rates, dynamic chang

摘要：

八仙花（Hydrangea macrophylla）是极具观赏价值的铝积累植物。铝离子作为八仙花花色调控的必要元素之一，对其蓝色花形成和植株的生长发育具有十分重要的作用。以‘Bailmer’为试验材料，确定其最适生长温度，并研究其体内铝离子的动态变化。结果表明，23 ℃有利于‘Bailmer’的生长发育及叶片和萼片对铝离子的吸收；花萼和叶片中铝离子含量的动态变化基本类似，均呈先上升、再下降、再上升的趋势；花萼与叶片的铝离子吸收速率均在前期迅速上升，在12 h时达到最大，而后又迅速降低，并趋于稳定。研究结果为八仙花花色调控奠定了理论基础。

关键词: 八仙花‘Bailmer’, 温度, 铝离子吸收速率, 动态变化

CLC Number:

S682

Zhiyun CHU, Hui QI, Ying LI, Yibing WAN, Zhiyu REN, Chun LIU, Suxia YUAN. Dynamic Changes of Aluminium Ion in the Sepals and Leaves of Hydrangea macrophylla ‘Bailmer’[J]. Journal of Agricultural Science and Technology, 2023, 25(1): 65-71.

褚志云, 祁惠, 李颖, 万一兵, 任志雨, 刘春, 袁素霞. 八仙花‘Bailmer’花萼和叶片内铝离子的动态变化[J]. 中国农业科技导报, 2023, 25(1): 65-71.

Figures/Tables 6

Fig. 1 Al ion content in sepal and leaf of ‘Bailmer’ under different temperaturesNote： Different small letters in the figure indicate significant difference at P<0.05 level.

Fig. 2 Chlorophyll fluorescence parameters in ‘Bailmer’ leaf under different temperaturesNote： Different small letters in the figure indicate significant difference at P<0.05 level.

Table 1 Plant growth index under different temperature

处理温度

Temperature/℃

株高

Plant height/mm

茎粗

Stem thickness/mm

叶长

Leaf length/mm

叶宽

Leaf width/mm

花径

Flower diameter/mm

Fig. 3 Plant growth under the different temperatures

Fig. 4 Dynamic change of Al ion content in sepals and leaves of ‘Bailmer’ after aluminum applicationNote：Different small letters in the figure indicate significant difference at P<0.05 level.

Fig. 5 Dynamic changes of Al ion absorption rate in sepal and leaf of ‘Bailmer’ after aluminum applicationNote： Different small letters in the figure indicate significant difference at P<0.05 level.

References 23

1	彭尽晖,周朴华,周红灿,等.湖南省绣球属植物资源调查[J].湖南农业大学学报(自然科学版),2008,34 (5):563-567.
	PENG J H, ZHOU P H, ZHOU H C, et al.. Investigation on Hydrangea resource in Hunan province [J]. J. Hunan Agric. Univ. (Nat. Sci.), 2008, 34 (5): 563-567.
2	曾奕,杨伟权,郁书君.绣球花的育种研究进展[J].广东农业科学,2018,45 (6):36-43.
	ZENG Y, YANG W Q, YU S J. Research progress of Hydrangea breeding [J]. Guangdong Agric. Sci., 2018, 45 (6): 36-43.
3	刘焕新.八仙花的花色调控[J].天津农林科技,2004 (5):21.
	LIU H X. The regulation of flower colour in Hydrangea macrophylla [J]. Sci. Technol. Tianjin Agric. Forestry, 2004 (5): 21.
4	ITO D, SHINKAI Y, KATO Y, et al.. Chemical studies on different color development in blue-and red-colored sepal cells of Hydrangea macrophylla [J]. Biosci. Biotechnol. Biochem., 2009, 73 (5): 1054-1059.
5	SCHREIBER H D, JONES A H, LARIVIERE C M, et al.. Role of aluminum in red-to-blue color changes in Hydrangea macrophylla sepals [J]. BioMetals, 2011, 24 (6): 1005-1015.
6	顾明华,陆申年,白厚义,等.铝毒在不同作物上的差异及温度的影响[J].热带亚热带土壤科学,1994,(2):59-65.
	GU M H, LU S N, BAI H Y, et al.. The difference of aluminum toxicity in different crops and the effect of temperature [J]. Trop. Subtrop. Soil Sci., 1994 (2): 59-65.
7	WAGATSUMA T. Effect of non-metabolic conditions on the uptake of aluminum by plant roots [J]. Soil Sci. Plant Nutr., 1983, 29 (3):323-333.
8	SILVA I R, SMYTH T J, MOXLEY D F, et al.. Aluminum accumulation at nuclei of cells in the root tip. Fluorescence detection using lumogallion and confocal laser scanning microscopy [J]. Plant Physiol., 2000, 123 (2): 543-552.
9	HUETT D O, MENARY R C. Aluminium Uptake by Excised Roots of Cabbage, Lettuce and Kikuyu Grass [J]. Funct. Plant Biol., 1979, 6 (6): 643-653.
10	NAUMANN A, HORST W J. Effect of aluminium supply on aluminium uptake, translocation and blueing of Hydrangea macrophylla (Thunb.) Ser. cultivars in a peatclay substrate [J]. J. Hortic. Sci. Biotechnol., 2003, 78 (4): 463-469.
11	HOTTA H, WANG Q, FUKUDA M, et al.. Identification of aluminum species in an aluminum-accumulating plant, hydrangea (Hydrangea macrophylla), by electrospray ionization mass spectrometry [J]. Anal. Sci., 2008, 24 (6): 795-798.
12	ITO T, AOKI D, FUKUSHIMA K, et al.. Direct mapping of Hydrangea blue-complex in sepal tissues of Hydrangea macrophylla [J/OL]. Sci. Rep., 2019, 9 (1): 5450 [2022-05-20]. .
13	杨锁宁.基质pH、铝离子对八仙花花色形成的调控机理[D].银川:宁夏大学,2020.
	YANG S N. The study on the mechanism of substrate pH and Al³⁺ regulating sepal color formation in Hydrangea macrophylla [D].Yinchuan: Ningxia University, 2020.
14	赵玉萍,邹志荣,白鹏威,等.不同温度对温室番茄生长发育及产量的影响[J]. 西北农业学报,2010,19 (2):133-137.
	ZHAO Y P, ZHOU Z R, BAI P W, et al.. Effect of different temperature on the growth and yield of tomato in greenhouse [J]. Acta Agric. Boreali-occident. Sin., 2010, 19 (2): 133-137.
15	SKALOVA H, MORAVCOVA L, DIXON A F G, et al.. Effect of temperature and nutrients on the growth and development of seedlings of an invasive plant [J]. Food Chem., 2016, 202: 184-188.
16	HUANG K L, MIYAJIMA I, OKUBO H. Effects of temperature and shade treatment on flower colors and characteristics in newly established reddish-purple tuberose(Polianthes) [J]. J. Faculty Agric. Kyushu Univ., 2000, 45 (1): 57-63.
17	杨锁宁,张黎,刘春,等.温度和土壤酸度对八仙花‘Bailmer’生长发育的影响[J].中国农业科技导报,2020,22 (5):24-34.
	YANG S N, ZHANG L, LIU C, et al.. Influences of temperature and soils acidity on the growth and development of Hydrangea macrophylla ‘Bailmer’ [J]. J. Agric. Sci. Technol., 2020, 22 (5): 24-34.
18	杨彬,金小青,陈修斌.温度对龙葵幼苗叶绿素含量和荧光参数的影响[J].安徽农业科学,2018,4605:56-57, 72.
	YANG B, JIN X Q, CHEN X B. Effects of different temperature on the content of chlorophyll in the leaves of Solanum nigrum L. seedlings and the fluorescent index [J]. J. Anhui Agric. Sci., 2018, 46(05): 56-57, 72.
19	HALDIMAN P, FRACHEBOUD Y, STAMP P. Photosynthetic performance and resistance to photoinhibition of Zea mays L. leaves grown at sub-optimal temperature [J]. Plant Cell Environ., 1996, 19: 85-92.
20	彭尽晖.湖南省绣球属(Hydrangea)植物资源及耐铝特性研究[D].长沙:湖南农业大学,2010.
	PENG J H. Study on resources of Hydrangea L . in Hunan Province and characteristics of aluminum tolerance [D]. Changsha: Hunan Agricultural University. 2010.
21	SHEN R F, IWASHITA T, MA J F. Form of Al changes with Al concentration in leaves of buckwheat [J]. J. Exp. Bot., 2004, 55 (394):131-136.
22	陈海霞,胡春梅,彭尽晖,等. 铝胁迫诱导八仙花根系分泌有机酸的研究[J].天津农业科学,2017,23 (2):1-7, 15.
	CHEN H X, HU C M, PENG J H, et al.. Al Stress-Induced Organic Acid Secretion from Roots in Hydrangea macrophylla [J]. Tianjin Agric. Sci., 2017, 23 (2): 1-7, 15.
23	MA J F. Role of organic acids in detoxification of Al in higher plants [J]. Plant Cell Physiol., 2000, 44: 383-390.

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